Susceptibility of mosquitoes to avian malaria

Huff, Clay G.

doi:10.1016/0014-4894(65)90036-6

Cited by 52 publications

(29 citation statements)

References 42 publications

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“…Neither oocysts nor sporozoites were observed at dissection under the microscope in C. pipiens pallens, and the domestic fowl bitten by 16 females on 12 days postfeeding was not infected with P. gallinaceum. These results with PCR detection correspond to those of previous studies that concluded that C. pipiens pallens is refractory to P. gallinaceum (Weathersby 1962;Huff 1965). …”

Section: Pcr Assay Of Abdomen and Thorax Of Infected Mosquitoessupporting

confidence: 94%

“…The general outline obtained by the PCR-based method for our laboratory experiment using A. aegypti and C. pipiens pallens for P. gallinaceum was consistent with previous studies using microscopy (Weathersby 1962;Huff 1965); thus, the results of PCR detection for parasites in the abdomen and thorax could be used as one of the criteria to assess susceptibility of mosquito species to Plasmodium parasite collected in the field. Although amplification of parasite DNA in a non-vector mosquito, C. pipiens pallens, was observed up to 13 days post-feeding, it was considered due to the detection of parasite DNA in a degenerating stage as in the case of A. albopictus infected by Plasmodium yoellii nigeriensis (Fabian et al 2004).…”

Section: Laboratory Verification Of Pcr Analysis For Blood-fed Mosquitomentioning

confidence: 74%

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Mosquito blood-meal analysis for avian malaria study in wild bird communities: laboratory verification and application to Culex sasai (Diptera: Culicidae) collected in Tokyo, Japan

et al. 2009

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We conducted laboratory experiments to verify molecular techniques of avian malaria parasite detection distinguishing between an infected mosquito (oocysts on midgut wall) and infective mosquito (sporozoites in salivary glands) in parallel with blood-meal identification from individual blood-fed mosquitoes prior to application to field survey for avian malaria. Domestic fowl infected with Plasmodium gallinaceum was exposed to a vector and non-vector mosquito species, Aedes aegypti and Culex pipiens pallens, respectively, to compare the time course of polymerase chain reaction (PCR) detection for parasite between competent and refractory mosquitoes. DNA of the domestic fowl was detectable for at least 3 days after blood feeding. The PCR-based detection of P. gallinaceum from the abdomen and thorax of A. aegypti corresponded to the microscopic observation of oocysts and sporozoites. Therefore, this PCR-based method was considered useful as one of the criteria to assess developmental stages of Plasmodium spp. in mosquito species collected in the field. We applied the same PCR-based method to 21 blood-fed C. sasai mosquitoes collected in Rinshi-no-mori Park in urban Tokyo, Japan. Of 15 blood meals of C. sasai successfully identified, 86.7% were avian-derived, 13.3% were bovine-derived. Plasmodium DNA was amplified from the abdomen of three C. sasai specimens having an avian blood meal from the Great Tit (Parus major), Pale Thrush (Turdus pallidus), and Jungle Crow (Corvus macrorhynchos). This is the first field study on host-feeding habits of C. sasai in relation to the potential role as a vector for avian malaria parasites transmitted in the Japanese wild bird community.

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Section: Pcr Assay Of Abdomen and Thorax Of Infected Mosquitoessupporting

confidence: 94%

Section: Laboratory Verification Of Pcr Analysis For Blood-fed Mosquitomentioning

confidence: 74%

Mosquito blood-meal analysis for avian malaria study in wild bird communities: laboratory verification and application to Culex sasai (Diptera: Culicidae) collected in Tokyo, Japan

et al. 2009

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“…While several species of anopheline and culicine mosquitoes have proven functional as experimental vectors of the avian species of Plasmodium, culicine mosquitoes appear to be more effective (Huff, 1965;Christensen et al, 1983;Nayar et al, 1998). Although the particular vectors of avian malaria in Madagascar are not known, 178 species of anopheline and culicine mosquitoes have been reported from the island, and more may remain to be identified.…”

Section: Discussionmentioning

confidence: 95%

A New Species of Plasmodium From Malagasy Vangas

Savage

Ariey

Greiner

2005

Journal of Parasitology

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During a recent examination of blood smears from Malagasy birds, a species of avian Plasmodium unlike those currently known was observed. All infected birds were members of the Vangidae, which is endemic to Madagascar and the Comoro Islands. Plasmodium parvulum n. sp. is described, and classified as a member of the subgenus Haemamoeba because of gametocyte and schizont shape, displacement of the host cell nucleus, as well as distortion of the host cell. Round, rosettelike schizonts with 6-8 merozoites, clumped refractile granules, and little cytoplasm were observed. Both schizonts and mature, round gametocytes rotated and displaced the erythrocyte nucleus. A brief comparison to P. relictum is included.

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“…Since the end of the 20th Century, a number of field and laboratory experimental infection studies were used to identify dipterans as vectors transmitting haemosporidian parasites (e.g. [7, 8]). Recently, most studies use molecular methods to identify vector feeding preferences [9] and sporogonic stages of the parasites in salivary glands to identify potential vectors [10].…”

Section: Introductionmentioning

confidence: 99%

Avian malaria on Madagascar: bird hosts and putative vector mosquitoes of different Plasmodium lineages

et al. 2017

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BackgroundAvian malaria occurs almost worldwide and is caused by Haemosporida parasites (Plasmodium, Haemoproteus and Leucocytozoon). Vectors such as mosquitoes, hippoboscid flies or biting midges are required for the transmission of these parasites. There are few studies about avian malaria parasites on Madagascar but none about suitable vectors.MethodsTo identify vectors of avian Plasmodium parasites on Madagascar, we examined head, thorax and abdomen of 418 mosquitoes from at least 18 species using a nested PCR method to amplify a 524 bp fragment of the haemosporidian mitochondrial cytochrome b gene. Sequences obtained were then compared with a large dataset of haemosporidian sequences detected in 45 different bird species (n = 686) from the same area in the Maromizaha rainforest.ResultsTwenty-one mosquitoes tested positive for avian malaria parasites. Haemoproteus DNA was found in nine mosquitoes (2.15%) while Plasmodium DNA was found in 12 mosquitoes (2.87%). Seven distinct lineages were identified among the Plasmodium DNA samples. Some lineages were also found in the examined bird samples: Plasmodium sp. WA46 (EU810628.1) in the Madagascar bulbul, Plasmodium sp. mosquito 132 (AB308050.1) in 15 bird species belonging to eight families, Plasmodium sp. PV12 (GQ150194.1) in eleven bird species belonging to eight families and Plasmodium sp. P31 (DQ839060.1) was found in three weaver bird species.ConclusionThis study provides the first insight into avian malaria transmission in the Maromizaha rainforest in eastern Madagascar. Five Haemoproteus lineages and seven Plasmodium lineages were detected in the examined mosquitoes. Complete life-cycles for the specialist lineages WA46 and P31 and for the generalist lineages mosquito132 and PV12 of Plasmodium are proposed. In addition, we have identified for the first time Anopheles mascarensis and Uranotaenia spp. as vectors for avian malaria and offer the first description of vector mosquitoes for avian malaria in Madagascar.

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Susceptibility of mosquitoes to avian malaria

Cited by 52 publications

References 42 publications

Mosquito blood-meal analysis for avian malaria study in wild bird communities: laboratory verification and application to Culex sasai (Diptera: Culicidae) collected in Tokyo, Japan

Mosquito blood-meal analysis for avian malaria study in wild bird communities: laboratory verification and application to Culex sasai (Diptera: Culicidae) collected in Tokyo, Japan

A New Species of Plasmodium From Malagasy Vangas

Avian malaria on Madagascar: bird hosts and putative vector mosquitoes of different Plasmodium lineages

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