Susceptibility of Listeria monocytogenes planktonic cultures and biofilms to sodium hypochlorite and benzalkonium chloride

Rodríguez-Melcón, Cristina; Riesco-Peláez, Félix; García, Fernández; Alonso‐Calleja, Carlos; Capita, Rosa

doi:10.1016/j.fm.2019.03.020

Cited by 49 publications

(33 citation statements)

References 29 publications

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“…In line with previous findings [19,[31][32][33][34][35], all the strains of Listeria tested showed a capacity to form biofilms under the conditions studied. Nevertheless, a strong strain-to-strain variation in biofilm-forming ability was observed (Figure 1).…”

Section: Discussionsupporting

confidence: 91%

“…An intra-specific variability in biofilm architecture has also been reported by other authors. Among the different structures seen are one-cell-thick layers, flat layers of cells consisting of several layers without a given structure, honeycomb structures, and networks of interwoven clumps [35][36][37][38][39][40][41][42]. The great variability observed in the structure of the biofilms formed by Listeria spp.…”

Section: Discussionmentioning

confidence: 99%

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Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials

2019

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Biofilms are a key factor in the persistence of Listeria in food processing plants, representing a potential source of foodstuff contamination. Nine Listeria strains (eight Listeria monocytogenes and one Listeria ivanovii) were studied by confocal laser scanning microscopy (CLSM) for their ability to form biofilm on glass, polystyrene, graphene and resin after 120 h of incubation at 12 °C. The relationship between cell surface hydrophobicity and biofilm formation was also investigated. On comparing the data for all the strains, similar (P > 0.05) biovolume values were obtained on glass (average 3.39 ± 1.69 µm3/µm2) and graphene (2.93 ± 1.14 µm3/µm2), while higher (P < 0.05) values were observed for polystyrene (4.39 ± 4.14 µm3/µm2). The highest (P < 0.01) biovolume levels were found in the biofilms formed on resin (7.35 ± 1.45 µm3/µm2), which also had the smallest biomass of inactivated cells (0.38 ± 0.37 µm3/µm2 vs. 1.20 ± 1.12 µm3/µm2 on the remaining surfaces; P < 0.001). No relationship was noted between cell surface hydrophobicity and biofilm-forming ability.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials

2019

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“…Among these, biofilm formation in polystyrene microtiter plates is certainly the most commonly used method [39,40]. The fact that all the strains assayed formed biofilms on polystyrene is a result coinciding with previous observations [14,15,41] and the findings of other authors [42,43]. This is a worrying discovery, because biofilms are a cause of contamination of food during processing; thus, representing a danger to public health.…”

Section: Capacity Of Strains Of L Monocytogenes To Form Biofilmsupporting

confidence: 71%

“…A relationship between biofilm formation and persistence has been suggested [13]. The sessile living state has been shown to be the preferred form of existence for many bacteria, and it is known that cells in biofilms show greater resistance to environmental challenges, such as desiccation, UV light, or sanitizers, than their planktonic counterparts in suspension [14,15]. This may lead to bacterial persistence in food-processing plants.…”

Section: Introductionmentioning

confidence: 99%

Persistent Listeria monocytogenes Isolates from a Poultry-Processing Facility Form More Biofilm but Do Not Have a Greater Resistance to Disinfectants than Sporadic Strains

et al. 2019

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Some strains of Listeria monocytogenes can persist in food-processing environments, increasing the likelihood of the contamination of foodstuffs. To identify traits that contribute to bacterial persistence, a selection of persistent and sporadic L. monocytogenes isolates from a poultry-processing facility was investigated for biofilm-forming ability (crystal violet assay). The susceptibility of sessile cells to treatments (five minutes) with sodium hypochlorite having 10% active chlorine (SHY: 10,000 ppm, 25,000 ppm, and 50,000 ppm) and benzalkonium chloride (BZK: 2500 ppm, 10,000 ppm, and 25,000 ppm) was also studied. All isolates exhibited biofilm formation on polystyrene. Persistent strains showed larger (p < 0.001) biofilm formation (OD580 = 0.301 ± 0.097) than sporadic strains (OD580 = 0.188 ± 0.082). A greater susceptibility to disinfectants was observed for biofilms of persistent strains than for those of sporadic strains. The application of SHY reduced biofilms only for persistent strains. BZK increased OD580 in persistent strains (2500 ppm) and in sporadic strains (all concentrations). These results indicate that the use of BZK at the concentrations tested could represent a public health risk. Findings in this work suggest a link between persistence and biofilm formation, but do not support a relationship between persistence and the resistance of sessile cells to disinfectants.

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“…The lower effectiveness of disinfection agents against bacterial biofilm compared to planktonic forms is a well-documented and undoubtful fact [26,32,[46][47][48][49]. It is speculated that the biofilm extracellular polymeric substances (EPSs) may serve as the primary target of ROS and protect the associated bacteria cells [32].…”

Section: Discussionmentioning

confidence: 99%

The Antimicrobial Effect of Radiant Catalytic Ionization on the Bacterial Attachment and Biofilm Formation by Selected Foodborne Pathogens under Refrigeration Conditions

Skowron

Bauza-Kaszewska

et al. 2020

Applied Sciences

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The decontamination of food contact surfaces is a major problem for the food industry. The radiant catalytic ionization (RCI) method, based on the ionization process, may be an alternative for conventional decontamination procedures. The advantage of this technique is the possibility of its application to household refrigerating appliances and industrial cold rooms. This study aimed to assess the effect of RCI on the reduction of Campylobacter jejuni, Listeria monocytogenes, and Salmonella Enteritidis from the biofilms formed on a glass surface under refrigeration conditions. Bacterial biofilms were exposed to RCI for 24 h and after 12 (variant I) and 72 h (variant II) of the glass surface contamination. In the last variant (III), the contaminated meat was placed on the glass surface in the refrigerator and subjected to RCI treatment for 72 h. The significantly highest values of absolute reduction efficiency coefficient E were found for the bacterial attachment stage of biofilm formation (variant I). The research proves the efficiency of the RCI method in the reduction of bacteria number from a glass surface.

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Susceptibility of Listeria monocytogenes planktonic cultures and biofilms to sodium hypochlorite and benzalkonium chloride

Cited by 49 publications

References 29 publications

Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials

Architecture and Viability of the Biofilms Formed by Nine Listeria Strains on Various Hydrophobic and Hydrophilic Materials

Persistent Listeria monocytogenes Isolates from a Poultry-Processing Facility Form More Biofilm but Do Not Have a Greater Resistance to Disinfectants than Sporadic Strains

The Antimicrobial Effect of Radiant Catalytic Ionization on the Bacterial Attachment and Biofilm Formation by Selected Foodborne Pathogens under Refrigeration Conditions

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