Susceptibility of isolates ofAcinetobacter anitratusandAcinetobacter lwoffiito the bactericidal activity of normal human serum

Jankowski, S.; Grzybek-Hryncewicz, K; Fleischer, Małgorzata; Walczuk, Maria

doi:10.1111/j.1574-6968.1992.tb05003.x

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…Previ ously, Jankowski et ai. [2] found that 12% of clinical isolates of Acinetobucter calcoaceticus and 84% of their Acinetobucter Iwoffii isolates were susceptible to the complement-mediated (alternatively and/or classically activated) bactericidal activity of human serum. These authors employed bacterial inocula of 10s CFU/ml at time 0, and strains that were killed 5^50% within 3 h were categorized as suscep tible.…”

Section: Discussionmentioning

confidence: 99%

“…These authors employed bacterial inocula of 10s CFU/ml at time 0, and strains that were killed 5^50% within 3 h were categorized as suscep tible. However, Jankowski et al [2] did not ex amine the two donor sera for antibody con tent. Our bacterial inocula were tenfold lower, and our assays included extended incubation.…”

Section: Discussionmentioning

confidence: 99%

“…I], Very little is known regarding host de fense mechanisms against this group of bacte ria [2][3][4], Preliminary investigations in our lab oratory revealed that most clinical isolates of diverse species of Acinetobacter were not com pletely killed by fresh defibrinated blood from human donors [5]. Additional studies showed that lipopolysaccharides and multiple poly peptides of selected strains of A. baumannii and of genospccies 3 were immunogenic for rabbits following experimentally induced bac teremia [6], This study served to examine se lected serologically carefully defined A. bau mannii and genospccies 3 isolates for suscepti bility to fresh defibrinated human blood used as such or following supplementation with 'natural' (intravenously applicable IgG prep arations) or 'immune' antibodies from pa tients with systemic infection due to Acineto bacter and after addition of rabbit immune sera.…”

Section: Introductionmentioning

confidence: 99%

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Exposure of Clinical Isolates of Acinetobacter baumanniiand Genospecies 3 to Defibrinated Human Blood with and without Added Human ‘Natural’ or (Patient) Immune Antibodies

Traub¹,

Leonhard²,

Bauer³

1995

Chemotherapy

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Fresh defibrinated blood (65% v/v) from 2 human donors failed to completely kill strains of Acinetobacter baumannii and genospecies 3, representing four serovars each. Although colony counts were reduced ≥90%, there invariably occurred rebound growth following extended incubation. Neither selected serovar-specific or isolate-homologous (following experimental bacteremia) rabbit immune sera nor three intravenously applicable IgG preparations augmented the bactericidal activity of human blood against six selected strains of A. baumannii and genospecies 3, despite documented antibody contents of all sera and IgG preparations employed. The majority of the sera from 22 patients who were early reconvalescent from systemic infection due to A. baumannii or genospecies 3, showed raised IgM antibody titers, and all sera demonstrated markedly elevated IgG antibody titers as determined with an enzyme-linked immunoassay. Defibrinated blood from donors B and T failed to completely kill the bacteria inocula of these 22 Acinetobacter isolates. Only one patient serum enhanced the bactericidal activity of human blood in the absence of antimicrobial drug(s), and only 1 patient serum with large amounts of detectable antimicrobial drug(s) effected complete killing of the homologous bacterial inoculum.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exposure of Clinical Isolates of Acinetobacter baumanniiand Genospecies 3 to Defibrinated Human Blood with and without Added Human ‘Natural’ or (Patient) Immune Antibodies

Traub¹,

Leonhard²,

Bauer³

1995

Chemotherapy

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An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii

2007

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Since the 1970s, the spread of multidrug-resistant (MDR) Acinetobacter strains among critically ill, hospitalized patients, and subsequent epidemics, have become an increasing cause of concern. Reports of community-acquired Acinetobacter infections have also increased over the past decade. A recent manifestation of MDR Acinetobacter that has attracted public attention is its association with infections in severely injured soldiers. Here, we present an overview of the current knowledge of the genus Acinetobacter, with the emphasis on the clinically most important species, Acinetobacter baumannii.

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