Susceptibility of inbred mouse strains to infection with Serpula (Treponema) hyodysenteriae

Abstract

Several inbred strains of mice were inoculated with Serpula (Treponema) hyodysenteriae B204 to determine susceptibility to infection. Challenge doses of 107 or 108 spirochetes induced cecal lesions in C3H/HeJ mice and other C3H strains of mice. However, more than a 100-fold difference existed between the dose required to induce lesions in 50% of the infected C3H/HeJ mice (8.3 x 107) and that required to induce them in 50% of the infected C3H/HeN mice (5 x 105). C3H/HeJ mice lack a splenocyte mitogenic response to Escherichia coli lipopolysaccharide but exhibited a mitogenic response comparable to those of other C3H strains of mice when stimulated with S. hyodysenteriae endotoxin (butanol-water extract). Different inbred strains exhibited different susceptibilities to infection, with the strain C3H/HeN being the most susceptible on the basis of colonization and development of macroscopic cecal lesions. The ity gene had no apparent effect on susceptibility of mice challenged with S. hyodysenteriae. The involvement of the H-2 haplotype with susceptibility is unclear, but the mice bearing H-2k were more susceptible than mice with the H-2b, H-2d, or H-2q haplotype. These data support the hypothesis that the host's responsiveness to lipopolysaccharide influences the susceptibility to infection with S. hyodysenteriae. However, differences in susceptibility between inbred mice exist independent of the Ips locus, suggesting that there are other inherent differences between mouse strains that affect susceptibility to infection by S. hyodysenteriae.

Characterization of two DNA probes specific for Serpulina hyodysenteriae

“…The preferred method for characterizing S. hyodysenteriae is sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of cellular proteins and Western blotting (immunoblotting) of the isolates with S. hyodysenteiaespecific antisera (25,26). However, restriction enzyme analysis (6), serotyping (2,20), and in vitro enteropathogenicity studies have also been used (12,15,21,28), but all these tests are time-consuming, labor-intensive, and expensive.…”

An enhanced murine model for studies of Serpulina (Treponema) hyodysenteriae pathogenesis

“…Murine cecal histopathological changes are similar to pathological changes observed in swine (10); however, clinical signs in the mouse are diminished when compared with those in swine. The mouse model has been used extensively in pathogenesis research of S. hyodysentenae (7, 10-12, 16-18, 20, 22) but the inconsistency of disease in the mouse model often makes interpretation of results difficult, especially for certain strains of mice (18). Differences in disease susceptibility have been described as a result of genetic differences (18,20) and the source of the mice (22).…”

The role of tumor necrosis factor and secondary bacteria in the pathogenesis of swine dysentery

“…In addition, S. hyodysenteriae could be readily reisolated from infected mice ceca at necropsy (12-15 days), but S. innocens was only shed for 7 to 10 days (42). While CF-1 mice were originally described as a model (37), inbred mice are also susceptible to development of lesions (61), however, susceptibilities do vary among different inbred strains (61). Congenitally athymic (nude) mice also appear susceptible to development of lesions after S. hyodysenteriae challenge (80).…”