Susceptibility of Inbred Mouse Strains to Infection With Three Species of Metagonimus Prevalent in the Republic of Korea

Abstract

Susceptibility of inbred mouse strains to Metagonimus yokogawai, Metagonimus miyatai, and Metagonimus takahashii infections was studied using BALB/c, ddY, C57BL/6J, C3H/HeN, and A/J mice, with H-2 haplotypes d, s, b, k, and a, respectively. Two hundred metacercariae were orally fed to each mouse, and the worm recovery rates (WRR), worm dimensions, and intrauterine egg numbers were measured at days 3, 7, 14, 21, and 28 postinfection (PI). On day 14 PI, the WRR of M. yokogawai was highest in ddY mice (average, 62.2%); those of M. miyatai and M. takahashii were highest in ddY (19.5%) and BALB/c mice (10.4%), respectively; worm maturation was best in C3H/HeN (M. yokogawai), C57BL/6J (M. miyatai), and ddY mice (M. takahashii). All mouse strains showed higher susceptibility to infection with M. yokogawai than with M. miyatai or M. takahashii. The results show that susceptibility of mice to Metagonimus infection varies according to mouse strain and parasite species but is suggested to be independent of the mouse H-2 haplotype.

Foodborne Intestinal Flukes in Southeast Asia

“…Freshwater fish, including the sweetfish, dace, common fat-minnow Morocco steindachneri, pale chub Zacco platypus, and dark chub Zacco temmincki, harbor the metacercariae [207]. Mice, rats, hamsters, and dogs are experimental definitive hosts [2,215]. Natural definitive hosts are unknown.…”

“…Freshwater fish, including the crussian carp C. carassius [219], carp C. carpio [217], dace Tribolodon taczanowskii [218], and perch Lateolabrax japonicus [221], harbor the metacercariae. Different strains of mice could be experimentally infected with fluke [215]. There are no reports on reservoir hosts.…”

“…Dogs, rats, and cats are the natural definitive hosts [88,102,229]. Variable mouse strains were found more susceptible to M. yokogawai infection and less susceptible to M. miyatai and M. takahashii infections [215].…”

Cd4+ T-cell-dependent Goblet Cell Proliferation and Expulsion of Gymnophalloides seoi from the Intestine of C57bl/6 Mice

“…Trematode study models include Echinostoma trivolvis, E. caproni, E. hortense, Neodiplostomun seoulense, Metagonimus yokogawai, and Gymnophalloides seoi. These trematodes were found to be spontaneously expelled from rodent intestines after days 3-21 post-infection (PI) (Fujino et al, 1996;Chai et al, 1998;Seo et al, 2003;Guk et al, 2005;Cho et al, 2007).…”

Mucosal Immune Responses of Mice Experimentally Infected with Pygidiopsis summa (Trematoda: Heterophyidae)

“…In intestinal trematode infections, such as Echinostoma spp. [14-16], Neodiplostomum seoulense [17-19], Metagonimus yokogawai [20-22], and Gymnophalloides seoi [23-25], innate intestinal immune mechanisms operate, and goblet cells, mast cells, intestinal intraepithelial lymphocytes (IEL), and/or mucosal IgA increased remarkably. However, in heterophyid fluke infections other than M. yokogawai , the role of these effectors has not been clarified.…”