Susceptibility of<i>Bonagota salubricola</i>(Lepidoptera: Tortricidae) to Insecticides in Brazilian Apple Orchards: Implications for Resistance Management

Bernardi, Daniel; Botton, Marcos; Andreazza, Felipe; Silva, Oscar Arnaldo Batista Neto e; Arioli, C. J.; Omoto, Celso

doi:10.1093/jee/tow143

Cited by 4 publications

(3 citation statements)

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“…Nonetheless, resistance of B. salubricola to tebufenozide was recently detected in Vacaria (BERNARDI et al, 2016). Nevertheless, it was surprising that the SS population had higher LC 50 than that of most field populations; the SS population was bred in laboratory for 18 years, and when they were collected in peach orchards in Italy, tebufenozide was no available for sale.…”

Section: Discussionmentioning

confidence: 99%

“…iv) Frequency of insecticide application. The number of insecticide applications has negative impact on natural enemies (MONTEIRO, 2001) and exerts selection pressure on tortricids (KANGA et al, 2003, SIEGWART et al, 2011, BERNARDI et al, 2016. The largest number of insecticides applied (more than 13) was considered a negative ecological impact (-3), and CC was -1 between 10 and 13 applications.…”

Section: Methodsmentioning

confidence: 99%

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Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil

et al. 2018

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-Oriental fruit moth (Grapholita molesta) is the most important pest of apple orchards in Brazil. For many years, insecticides have been the only tool used for its control. The aim of this work was to characterize the management of apple orchards by the system for Assessment of Environment Impact of Agricultural Technology Innovations (AMBITEC-AGRO) and to relate it with the resistance status of G. molesta populations. Insecticide resistance was estimated by toxicology and enzymatic activities of glutathione S-transferase (GST), esterases (α-β EST), and acetylcholinesterase (AChE). The phytosanitary strategies adopted by the four apple production orchards in southern Brazil were analyzed in: Vacaria (RS population), Fraiburgo (SC1 population), São Joaquim (SC2 population), and Porto Amazonas (PR population). Oriental fruit moth field populations were reared in laboratory for bioassays with chlorpyriphos, carbaryl, deltamethrin, and tebufenozide insecticides and were compared with a reference susceptible population. Larval mortality rates were recorded for seven days. GST, α-β EST, and AChE assays were performed with third-and fourth-instar larvae. Results indicated that oriental fruit moth populations collected in Vacaria, Fraiburgo, and São Joaquim showed tolerance to carbaryl and chlorpyriphos, whereas the population collected in Porto Amazonas was more susceptible. SC2 and PR populations were more tolerant to deltamethrin, while RS and SC1 populations were more susceptible. No population was tolerant to tebufenozide. RS population showed the highest activity for AChE, GST, and α-EST. The findings of this study suggest that the AMBITEC-AGRO system was a suitable method to evaluate the phytosanitary activity of apple orchards and to relate it to the resistance status of G. molesta populations. Index Terms: Resistance management; Grapholita molesta; susceptibility, apple orchard Resistência de mariposa oriental (Lepidoptera: Tortricidae) a inseticidas em pomares de maçã no sul do BrasilResumo-Mariposa-oriental (Grapholita molesta) é a praga mais importante de pomares de maçã no Brasil. Por muitos anos, o inseticida foi a única ferramenta usada como controle. O objetivo deste trabalho foi caracterizar o manejo dos pomares por meio da Avaliação de Impacto Ambiental da Inovação Tecnológica Agropecuária (AMBITEC-AGRO) e relacionar com o status da resistência de populações de G. molesta nos pomares de maçã, por bioensaios toxicológicos e atividades enzimáticas de glutatione S-transferase (GST), esterases (α-β EST) e acetilcolinesterase (AChE). A estratégia fitossanitária adotada por quatro pomares de maçã no Sul do Brasil foi analisada em: Vacaria (população RS), Fraiburgo (população SC1), São Joaquim (população SC2) e Porto Amazonas (população PR). As populações foram criadas em laboratório para bioensaios com clorpirifós, carbaril, deltametrina e tebufenozide. A mortalidade larval foi avaliada durante sete dias. A atividade das enzimas GST, α-βEST e AChE foi realizada com larvas de terceiro e quarto instar...

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil

et al. 2018

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“…The estimate of baselines and monitoring of susceptibility of G. molesta to insecticides provide information to the establishment of proactive IRM program as verified to Bonagota salubricola (Meyrick) (Lepidoptera: Tortricidae) in apple orchards in Brazil (BERNARDI et al, 2016). The initiative also allowed monitoring changes in pest susceptibility over time and define IRM strategies,; for example, in the rotation of insecticides with different modes of action (ROUSH & MILLER, 1986;SPARKS & NAUEN, 2015).…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Grapholita molesta to insecticides in Brazil

Silva¹,

Botton

Bernardi

et al. 2017

Cienc. Rural

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The use of insecticides has been the main tool for Grapholita molesta (Busck) control in Brazil, which is considered one of the most important pests in apple and peach orchards. In order to implement an Insect Resistance Management (IRM) program, studies were conducted to characterize the baseline susceptibility of G. molesta to major insecticides for its control. Then, we conducted an insecticide susceptibility monitoring in thirteen field-collected populations of the pest. Neonates (0-24h old) were exposed to insecticides applied on surface of artificial diet. A high susceptibility was verified when neonates of the Laboratory population of G. molesta were exposed to insecticides with LC50 values (µg a.i./cm2) of 0.1 (spinetoram), 1.0 (metaflumizone), 1.2 (chlorantraniliprole), 4.8 (novaluron), 5.1 (tebufenozide), 11.3 (phosmet) and 222.5 (pyriproxyfen). Based on the LC99 (µg a.i./cm2), the diagnostic concentrations of 0.6 (spinetoram), 5.5 (metaflumizone), 5.6 (chlorantraniliprole), 19.6 (tebufenozide), 37.4 (phosmet), 37.8 (novaluron) and 2011 pyriproxyfen) caused high mortality (>95%) of neonates from field populations. These diagnostic concentrations will be used in resistance monitoring programs of G. molesta in Brazil.

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Evaluation of Resistance Management for the Oriental Fruit Moth (Lepidoptera: Tortricidae) to Insecticides in Brazilian Apple Orchards

Monteiro

Witt

Guiloski

et al. 2020

Journal of Economic Entomology

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The oriental fruit moth, Grapholita molesta (Busk, 1916), is one of the most important pests in apple orchards in southern Brazil. Chemical control is still the most commonly used strategy for pest control. The aim of this study was to determine the resistance evolution of seven South Brazilian populations to four insecticides (chlorantraniliprole, lufenuron, chlorpyrifos, and fenitrothion). Bioassays were paired with metabolic analyses of glutathione-S-transferase (GST), carboxylesterases (α-NA and β-NA), and acetylcholinesterase (AChE) to understand the possible role with phytosanitary strategies in the insects’ susceptibility. Insect populations were collected in the municipalities of Campo do Tenente, Lapa and Porto Amazonas, Fraiburgo, São Joaquim, and Vacaria and multiplied in the laboratory. Two susceptible and two resistant populations were used as references. The bioassays showed that five populations were considered more resistant to organophosphates, six to lufenuron and two to chlorantraniliprole when compared with the sensitive population. None of the field populations had greater resistance than the resistant laboratory population. The enzymatic activity of AChE and GST was elevated in most of the populations that were less susceptible to organophosphates and lufenuron. The populations originating from orchards that used sexual confusion techniques had the greatest susceptibility based on toxicological and biochemical bioassays. Populations under pressure from various compounds had high GST, α and β-NA activity. There is evidence that a diversity of control strategies can provide better resistance management.

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Susceptibility ofBonagota salubricola(Lepidoptera: Tortricidae) to Insecticides in Brazilian Apple Orchards: Implications for Resistance Management

Cited by 4 publications

References 21 publications

Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil

Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil

Susceptibility of Grapholita molesta to insecticides in Brazil

Evaluation of Resistance Management for the Oriental Fruit Moth (Lepidoptera: Tortricidae) to Insecticides in Brazilian Apple Orchards

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