Susceptibility of human cells to Puumala virus infection

Temonen, Mari; Vapalahti, Olli; Holthöfer, Harry; Brummer-Korvenkontio, M; Vaheri, Antti; Lankinen, Hilkka

doi:10.1099/0022-1317-74-3-515

Cited by 90 publications

(72 citation statements)

References 22 publications

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“…The well-defined endothelial specialization and heterogeneity are not only apparent in the tropism of pathogens, many human vascular diseases are limited to distinct types of vessels, e.g., autoimmune diseases that mainly manifest in the kidney (34). The strong association between disease and cell type demands the investigation of the underlying molecular pathomechanism in a cell culture model relating to the relevant target organ (79). We have shown that the hantavirus receptor integrin ␣ V ␤ 3 is expressed on tubular and glomerular cells of the human kidney and that podocytes and glomerular endothelial and tubular epithelial cells are permissive to infection and release infectious particles.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts

Krautkrämer

Grouls

Stein

et al. 2011

J Virol

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Viral hemorrhagic fevers are characterized by enhanced permeability. One of the most affected target organs of hantavirus-induced hemorrhagic fever with renal syndrome is the kidney, and an infection often results in acute renal failure. To study the underlying cellular effects leading to kidney dysfunction, we infected human renal cell types in vitro that are critical for the barrier functions of the kidney, and we examined kidney biopsy specimens obtained from hantavirus-infected patients. We analyzed the infection and pathogenic effects in tubular epithelial and glomerular endothelial renal cells and in podocytes. Both epithelial and endothelial cells and podocytes were susceptible to hantavirus infection in vitro. The infection disturbed the structure and integrity of cell-to-cell contacts, as demonstrated by redistribution and reduction of the tight junction protein ZO-1 and the decrease in the transepithelial resistance in infected epithelial monolayers. An analysis of renal biopsy specimens from hantavirus-infected patients revealed that the expression and the localization of the tight junction protein ZO-1 were altered compared to renal biopsy specimens from noninfected individuals. Both tubular and glomerular cells were affected by the infection. Furthermore, the decrease in glomerular ZO-1 correlates with disease severity induced by glomerular dysfunction. The finding that different renal cell types are susceptible to hantaviral infection and the fact that infection results in the breakdown of cell-to-cell contacts provide useful insights in hantaviral pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Pathogenic Old World Hantaviruses Infect Renal Glomerular and Tubular Cells and Induce Disassembling of Cell-to-Cell Contacts

Krautkrämer

Grouls

Stein

et al. 2011

J Virol

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“…To determine whether the 1918 virus genes responsible for the high virulence in vivo also contribute to optimal virus replication in human airway cells, we assessed the growth and release of virus in primary human bronchial epithelial (NHBE) cells (25) and in Detroit 562 epithelial cells derived from a human pharyngeal carcinoma (26). Culture medium from virus-inoculated NHBE cells was collected from the apical and basolateral chambers of the cell monolayers at different times after inoculation and examined for virus production in the presence and absence of trypsin.…”

Section: Construction and Characterization Of Recombinant Viruses Witmentioning

confidence: 99%

“…Primary human bronchial epithelial (NHBE) cells (Cambrex Bio Science) (25) and Detroit 562 epithelial cells (American Type Culture Collection) (26), were grown in MEM as described in ref. 25.…”

Section: Infection Of Micementioning

confidence: 99%

Single gene reassortants identify a critical role for PB1, HA, and NA in the high virulence of the 1918 pandemic influenza virus

Pappas

Aguilar²,

Basler³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

143

142

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“…Other cell lines that support the growth of hantaviruses include hybridoma cells [42], primary human adult endothelial cells [43], primary human umbilical vein endothelial cells [44], murine macrophage-like continuous cell lines [45], primary rat peritoneal exudate cells and primary rat macrophages and primary human adherent mononuclear cells [46], a large number of human continuous cell lines of lung, kidney, liver and salivary origin and primary human kidney cells [47]. There is no evidence of CPE in any of these cell types.…”

Section: Growth In Cell Culturementioning

confidence: 99%