Susceptibility of hamsters to infection by historic and epidemic BI Clostridium difficile strains during daily administration of three fluoroquinolones

Phillips, S. Tyler; Nagaro, Kristin; Sambol, Susan P.; Gerding, Dale N.

doi:10.1016/j.anaerobe.2011.03.015

Cited by 11 publications

(9 citation statements)

References 8 publications

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“…In addition, ciprofloxacin and levofloxacin also exerted selective pressure in the mouse when given in combination with other antianaerobic antibiotics. We showed in a hamster model of CDI that an epidemic BI strain (BI17) with high-level fluoroquinolone resistance had similar colonization rates following treatment with ciprofloxacin, levofloxacin, and moxifloxacin (15). In contrast, an historic BI strain (BI1) that was not resistant to moxi- floxacin colonized hamsters efficiently only after moxifloxacin treatment, supporting acquisition of high-level fluoroquinolone resistance as a risk for CDI for all fluoroquinolones.…”

Section: Discussionmentioning

confidence: 94%

Fluoroquinolone and Macrolide Exposure Predict Clostridium difficile Infection with the Highly Fluoroquinolone- and Macrolide-Resistant Epidemic C. difficile Strain BI/NAP1/027

Wieczorkiewicz

Lopansri

Cheknis

et al. 2016

Antimicrob Agents Chemother

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Section: Discussionmentioning

confidence: 94%

Fluoroquinolone and Macrolide Exposure Predict Clostridium difficile Infection with the Highly Fluoroquinolone- and Macrolide-Resistant Epidemic C. difficile Strain BI/NAP1/027

Wieczorkiewicz

Lopansri

Cheknis

et al. 2016

Antimicrob Agents Chemother

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“…In other words, some antibiotics with activity against C. difficile (e.g., metronidazole) may prevent or delay CDI despite disrupting the microbial flora [ 13 ],[ 14 ]. Loss of activity of fluoroquinolones against C. difficile and the associated increase of CDI suggest that antibiotic activity against C. difficile may, at least partially, hinder proliferation and toxin production despite disrupting the GI flora [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic susceptibility of Clostridium difficile is similar worldwide over two decades despite widespread use of broad-spectrum antibiotics: an analysis done at the University Hospital of Zurich

et al. 2014

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BackgroundClostridium difficile infection (CDI) remains a major health problem worldwide. Antibiotic use, in general, and clindamycin and ciprofloxacin, in particular, have been implicated in the pathogenesis of CDI. Here, we hypothesized that antibiotics that are highly active in vitro against C. difficile are less frequently associated with CDI than others. The primary goals of our study were to determine if antibiotic susceptibility and CDI are associated and whether the antimicrobial susceptibility of C. difficile changed over the years.Methods and resultsWe examined a large panel of C. difficile strains collected in 2006-2008 at the University Hospital of Zurich. We found that the antimicrobial susceptibilities to amoxicillin/clavulanate, piperacillin/tazobactam, meropenem, clindamycin, ciprofloxacin, ceftriaxone, metronidazole and vancomycin were similar to those reported in the literature and that they are similar to those reported in other populations over the last two decades. Antibiotic activity did not prevent CDI. For example, thre use of meropenem, which is highly active against all strains tested, was a clear risk factor for CDI. Most of the antibiotics tested also showed a higher minimum inhibitory concentration distribution than that of EUCAST. All strains were susceptible to metronidazole. One strain was resistant to vancomycin.ConclusionsAntibiotic susceptibilities of the collection of C. difficile from the University Hospital of Zurich are similar to those reported by others since the 1980. Patients treated with carbapenems and cephalosporins had the highest risk of developing CDI irrespective of the antimicrobial activity of carbapenems.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-014-0607-z) contains supplementary material, which is available to authorized users.

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“…Hamster model of antibiotic-induced C. difficile infection. A previously developed hamster model of antibiotic-induced C. difficile infection was adapted to moxifloxacin (a fluoroquinolone antibiotic) and clindamycin (a lincosamide antibiotic) (27). After an 8-day acclimation period, male golden Syrian hamsters (80 to 120 g) received the antibiotic by subcutaneous injection at a time designated H 0 once a day from day 1 (D 1 ) to day 5 (D 5 ).…”

Section: Methodsmentioning

confidence: 99%

Antibiotic-Induced Dysbiosis Predicts Mortality in an Animal Model of Clostridium difficile Infection

Burdet

Sayah-Jeanne

Nguyễn

et al. 2018

Antimicrob Agents Chemother

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Antibiotic disruption of the intestinal microbiota favors colonization by Using a charcoal-based adsorbent to decrease intestinal antibiotic concentrations, we studied the relationship between antibiotic concentrations in feces and the intensity of dysbiosis and quantified the link between this intensity and mortality. We administered either moxifloxacin ( = 70) or clindamycin ( = 60) to hamsters by subcutaneous injection from day 1 (D) to D and challenged them with a toxigenic strain at D Hamsters received various doses of a charcoal-based adsorbent, DAV131A, to modulate intestinal antibiotic concentrations. Gut dysbiosis was evaluated at D and D using diversity indices determined from 16S rRNA gene profiling. Survival was monitored until D We analyzed the relationship between fecal antibiotic concentrations and dysbiosis at the time of challenge and studied their capacity to predict subsequent death of the animals. Increasing doses of DAV131A reduced fecal concentrations of both antibiotics, lowered dysbiosis, and increased survival from 0% to 100%. Mortality was related to the level of dysbiosis ( < 10 for the change of Shannon index in moxifloxacin-treated animals and < 10 in clindamycin-treated animals). The Shannon diversity index and unweighted UniFrac distance best predicted death, with areas under the receiver operating curve (ROC) of 0.89 (95% confidence interval [CI], 0.82, 0.95) and 0.95 (0.90, 0.98), respectively. Altogether, moxifloxacin and clindamycin disrupted the diversity of the intestinal microbiota with a dependency on the DAV131A dose; mortality after challenge was related to the intensity of dysbiosis in similar manners with the two antibiotics.

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Susceptibility of hamsters to infection by historic and epidemic BI Clostridium difficile strains during daily administration of three fluoroquinolones

Cited by 11 publications

References 8 publications

Fluoroquinolone and Macrolide Exposure Predict Clostridium difficile Infection with the Highly Fluoroquinolone- and Macrolide-Resistant Epidemic C. difficile Strain BI/NAP1/027

Fluoroquinolone and Macrolide Exposure Predict Clostridium difficile Infection with the Highly Fluoroquinolone- and Macrolide-Resistant Epidemic C. difficile Strain BI/NAP1/027

Antibiotic susceptibility of Clostridium difficile is similar worldwide over two decades despite widespread use of broad-spectrum antibiotics: an analysis done at the University Hospital of Zurich

Antibiotic-Induced Dysbiosis Predicts Mortality in an Animal Model of Clostridium difficile Infection

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