Susceptibility of Genital Mycoplasmas to Antimicrobial Agents

Braun, Peter; Klein, Jerome O.; Kass, Edward H.

doi:10.1128/aem.19.1.62-70.1970

Cited by 55 publications

(7 citation statements)

References 24 publications

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“…The r6le of M. hominis in the etiology of bacterial vaginosis remains unsettled. Although M. hominis has been reported to be resistant to erythromycin and metronidazole (12,13), in the present study we found M. hominis to have been eradicated in all women who reported themselves to be symptom-free and who had no signs of bacterial vaginosis at check-up. The effectiveness of the antibiotics may be due to active metabolites against the organism.…”

Section: Discussioncontrasting

confidence: 55%

Erythromycin Versus Metronidazole in the Treatment of Bacterial Vaginosis

Wathne

Holst

Hovelius

et al. 1994

Obstetrical & Gynecological Survey

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Of 101 women, 15-50 years of age, presenting with vaginal discharge, 34 had bacterial vaginosis and were randomly assigned to a seven-day course of oral treatment with either erythromycin (0.5 g b i d . ) or metronidazole (0.4 g b.i.d.) in a single-blind, cross-over study. Treatment failure (?three clinical signs of bacterial vaginosis) occurred in 13 (81%) of 16 patients given erythromycin, as compared with three (17%) of 18 women treated with metronidazole QJ < 0.001). Persistence of Gardnerella vaginalis, Mobiluncus species and/or Mycoplasma hominis was found in 14 of 16 patients treated with erythromycin, and in four of 16 patients treated with metronidazole. Treatment with metronidazole was successful ( 2 two clinical signs of bacterial vaginosis) in eight of 10 cases of erythromycin treatment failure. Neither of two cases of metronidazole treatment failure was cured with erythromycin. At three-month follow-up of 3 1 women, persistence or recurrence of bacterial vaginosis was diagnosed in 11 cases (36%)).

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Section: Discussioncontrasting

confidence: 55%

Erythromycin Versus Metronidazole in the Treatment of Bacterial Vaginosis

Wathne

Holst

Hovelius

et al. 1994

Obstetrical & Gynecological Survey

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“…Highly differing isolation rates for mycoplasmas have been published for male animals of different species, ranging from 27 to 72% in dogs (Doig et al 1981;Bjurstr€ om and Linde-Forsberg 1992;Sch€ afer-Somi et al 2009), 0-80% in horses (Spergser et al 2002;Baczynska et al 2007) and <10% in bulls located on different AI centres (Petit et al 2008). Low isolation rates may be a consequence to the fact that not all studies were performed using specific culture media which have been shown crucial to obtain growth of mycoplasmas in vitro (Braun et al 1970;Chalker 2005;Baczynska et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Low isolation rates may be a consequence to the fact that not all studies were performed using specific culture media which have been shown crucial to obtain growth of mycoplasmas in vitro (Braun et al. ; Chalker ; Baczynska et al. ).…”

Section: Discussionmentioning

confidence: 99%

Cooled Storage of Canine Semen:in vitroEffects of Different Concentrations of an Antibiotic Combination on Growth of Mollicutes

Becher¹,

Spergser

Aurich³

et al. 2013

Reprod Domestic Animals

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The aim of this study was to examine effects of an antibiotic combination at different concentrations on growth of mycoplasma and ureaplasma during cooled storage of canine semen (n = 20). Semen aliquots were diluted with Tris-citric acid-fructose-egg yolk extender containing either 1.0 g/l streptomycin and 0.6 g/l benzylpenicillin (control) or a combination of gentamycin, tylosin, lincomycin and spectinomycin (GTLS-1: 0.25, 0.05, 0.15 and 0.3; GTLS-2: 0.5, 0.1, 0.3 and 0.6; GTLS-3: 1.0, 0.2, 0.6 and 1.2 g/l). Samples were assessed for motility and membrane integrity by computer-assisted sperm analysis immediately after dilution and at 24, 48 and 72 h of cooled storage. Morphologically, normal spermatozoa were determined, and bacterial culture was performed at 24 and 72 h. Mycoplasma spp. were detected in 14 of 20 ejaculates (70%) with severe growth in 12 samples. A reduction but not total elimination of mycoplasma growth occurred in all GTLS extenders with the most pronounced reduction in group GTLS-3 (control vs GTLS-1 and GTLS-2 p < 0.05, control vs GTLS-3 p < 0.001). Ureaplasmas were detected in four ejaculates, and growth was reduced to the same extent in GTLS and control extender. Progressive motility in all groups, total motility in groups GTLS 1-3 and percentage of membrane-intact spermatozoa in groups GTLS 2 and 3 decreased slightly (p < 0.05) over time. In conclusion, dilution of canine semen with GTLS extender has no major detrimental effects on spermatozoa during cooled storage. It reduced the growth but did not totally eliminate mycoplasmas and ureaplasmas from cooled-stored dog semen.

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“…These concentrations have been shown to be inhibitory for ureaplasmas (1,14). CFU were determined by the standard plate dilution technique using Shepard's urease test reagent (9) to help see the colonies.…”

mentioning

confidence: 99%

Effect of antibiotics on the dynamics of color change in Ureaplasma urealyticum cultures

Bloomster¹,

Lynn²

1981

J Clin Microbiol

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Color change of pH indicators in broth medium is commonly used to quantify growth of ureaplasmas. These organisms differ from other members of the Mollicutes by their abiity to hydrolyze urea to C02 and NH3. This study describes a method which continuously monitors color change in ureaplasmal broth cultures. Using this technique we found: (i) there was a pH-dependent absorbance at 554 nm in ureaplasmal broth medium containing phenol red, (ii) a sigmoidalshaped color changing curve (absorbance at 554 nm versus time) was produced by metabolizing organisms whereas a linear curve was generated by antibioticinhibited ureaplasmas, and (iii) the minimum cell density which elicited a growthinhibited color change was 1.25 x 104 colony-forming units per ml. Others have shown that apparently dead ureaplasmas can cause a color change in broth media. This color change is probably due to the presence of an active urease. This study graphically and quantitatively assesses growth-inhibited color change.

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Susceptibility of Genital Mycoplasmas to Antimicrobial Agents

Cited by 55 publications

References 24 publications

Erythromycin Versus Metronidazole in the Treatment of Bacterial Vaginosis

Erythromycin Versus Metronidazole in the Treatment of Bacterial Vaginosis

Cooled Storage of Canine Semen:in vitroEffects of Different Concentrations of an Antibiotic Combination on Growth of Mollicutes

Effect of antibiotics on the dynamics of color change in Ureaplasma urealyticum cultures

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