Susceptibility of Florida Mosquitoes to Infection with Chikungunya Virus

Reiskind, Michael H.; Pesko, Kendra N.; Westbrook, Catherine J.; Mores, Christopher N.

doi:10.4269/ajtmh.2008.78.422

Cited by 69 publications

(71 citation statements)

References 28 publications

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“…It should be underlined that we are not able to provide a control of salivation, and we hypothesize that a CHIKV-negative saliva sample did not correspond to mosquitoes unable to salivate but to mosquitoes delivering noninfected saliva. As expected from previous studies (22,25,30,41), A. albopictus better transmitted the epidemic strain CHIKV_0621 of the ECSA genotype than A. aegypti, even in cases where both mosquito species cohabit. A. aegypti transmitted preferentially CHIKV_115 and also the Asian genotype CHIKV_NC in accordance with previous findings (28).…”

Section: Discussionsupporting

confidence: 68%

“…The CHIKV lineages have displayed distinct transmission efficiencies in mosquito vector species and populations (25,28,29). Throughout the 2005-2006 CHIKV epidemic in the Indian Ocean region, a CHIKV lineage strain harboring a substitution of an alanine to valine at position 226 of the E1 envelope glycoprotein (E1-A226V) was better transmitted by A. albopictus (22,25,30). It was later shown that other positions in the E2 glycoprotein exert epistatic effects on the position E1-226V (23,24), and some substitutions can block the adaptation of E1-226V to A. albopictus.…”

Section: Hikungunya Virus (Chikv) Is An Alphavirus In the Familymentioning

confidence: 99%

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High Level of Vector Competence of Aedes aegypti and Aedes albopictus from Ten American Countries as a Crucial Factor in the Spread of Chikungunya Virus

Vega-Rúa

Zouache

Girod

et al. 2014

J Virol

375

357

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Chikungunya virus (CHIKV) causes a major public health problem. In 2004, CHIKV began an unprecedented global expansion and has been responsible for epidemics in Africa, Asia, islands in the Indian Ocean region, and surprisingly, in temperate regions, such as Europe. Intriguingly, no local transmission of chikungunya virus (CHIKV) had been reported in the Americas until recently, despite the presence of vectors and annually reported imported cases. Here, we assessed the vector competence of 35 American Aedes aegypti and Aedes albopictus mosquito populations for three CHIKV genotypes. We also compared the number of viral particles of different CHIKV strains in mosquito saliva at two different times postinfection. Primarily, viral dissemination rates were high for all mosquito populations irrespective of the tested CHIKV isolate. In contrast, differences in transmission efficiency (TE) were underlined in populations of both species through the Americas, suggesting the role of salivary glands in selecting CHIKV for highly efficient transmission. Nonetheless, both mosquito species were capable of transmitting all three CHIKV genotypes, and TE reached alarming rates as high as 83.3% and 96.7% in A. aegypti and A. albopictus populations, respectively. A. albopictus better transmitted the epidemic mutant strain CHIKV_0621 of the East-Central-South African (ECSA) genotype than did A. aegypti, whereas the latter species was more capable of transmitting the original ECSA CHIKV_115 strain and also the Asian genotype CHIKV_NC. Therefore, a high risk of establishment and spread of CHIKV throughout the tropical, subtropical, and even temperate regions of the Americas is more real than ever.

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Section: Discussionsupporting

confidence: 68%

Section: Hikungunya Virus (Chikv) Is An Alphavirus In the Familymentioning

confidence: 99%

High Level of Vector Competence of Aedes aegypti and Aedes albopictus from Ten American Countries as a Crucial Factor in the Spread of Chikungunya Virus

Vega-Rúa

Zouache

Girod

et al. 2014

J Virol

375

357

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“…This population of Ae. albopictus was previously shown to be highly susceptible to the La Réunion (LR2006-OPY1) CHIKV strain (Reiskind et al 2008). Females reared from field-collected immatures were given 20% sucrose ad libitum, blood fed weekly on live chickens, and kept in cages at 14 L:10 D, 268C (AE18C SD) and >80% relative humidity (rh).…”

Section: Mosquitoes and Virusesmentioning

confidence: 99%

Larval Environmental Temperature and the Susceptibility ofAedes albopictusSkuse (Diptera: Culicidae) to Chikungunya Virus

Westbrook

Reiskind

Pesko

et al. 2010

Vector-Borne and Zoonotic Diseases

Self Cite

127

125

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A key feature in the recent widespread epidemic of the mosquito-borne alphavirus chikungunya virus (CHIKV) was the important role of Aedes albopictus, formerly regarded as a secondary vector, compared to the presumed primary vector Aedes aegypti. Ae. albopictus, a container-inhabiting mosquito, is an invasive species that occurs over a wide geographic range spanning tropical and temperate latitudes. In this study we examine the effects of a broad range of larval rearing temperatures on CHIKV infection, dissemination, and viral titer in Florida F(1) Ae. albopictus. Adults from larvae reared at 18 degrees C, 24 degrees C, and 32 degrees C differed significantly in size, development time, and CHIKV infection rate. Adult females with the largest body size were produced from the coolest temperature, took the longest to mature, and six times more likely to be infected with CHIKV than females reared at 32 degrees C. There was also a significant effect of rearing temperature on viral dissemination, resulting in an increase in population dissemination at the coolest temperature. This study indicates that climate factors, such as temperature, experienced at the larval stage, can influence the competence of adult females to vector arboviruses.

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“…Recent outbreaks caused millions of CHIKV infections in south India and on La Reunion and other islands in the Indian Ocean (3,4). Although past epidemics of CHIKV have been confined to Africa and Asia, increased air travel has produced increased transmission in Europe and the Western hemisphere (4)(5)(6). A mutation enhancing the ability of CHIKV to infect and be transmitted by the Aedes albopictus mosquito has also increased the risk for infection due to the continuing global spread of this vector (7)(8)(9).…”

mentioning

confidence: 99%

Cross-Inhibition of Chikungunya Virus Fusion and Infection by Alphavirus E1 Domain III Proteins

Martín

Nanda

Zheng

et al. 2013

J Virol

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Alphaviruses are small enveloped RNA viruses that include important emerging human pathogens, such as chikungunya virus (CHIKV). These viruses infect cells via a low-pH-triggered membrane fusion reaction, making this step a potential target for antiviral therapies. The E1 fusion protein inserts into the target membrane, trimerizes, and refolds to a hairpin-like conformation in which the combination of E1 domain III (DIII) and the stem region (DIII-stem) pack against a core trimer composed of E1 domains I and II (DI/II). Addition of exogenous DIII proteins from Semliki Forest virus (SFV) has been shown to inhibit E1 hairpin formation and SFV fusion and infection. Here we produced and characterized DIII and DI/II proteins from CHIKV and SFV. Unlike SFV DIII, both core trimer binding and fusion inhibition by CHIKV DIII required the stem region. CHIKV DIIIstem and SFV DIII-stem showed efficient cross-inhibition of SFV, Sindbis virus, and CHIKV infections. We developed a fluorescence anisotropy-based assay for the binding of SFV DIII-stem to the core trimer and used it to demonstrate the relatively high affinity of this interaction (K d [dissociation constant], ϳ85 nM) and the importance of the stem region. Together, our results support the conserved nature of the key contacts of DIII-stem in the alphavirus E1 homotrimer and describe a sensitive and quantitative in vitro assay for this step in fusion protein refolding. The Alphavirus genus contains about 40 recognized species of small enveloped plus-sense RNA viruses (1, 2). Most alphaviruses are transmitted by mosquito vectors and can infect a variety of mammalian, avian, and other hosts. Alphaviruses can produce fever, arthritis, and encephalitis in humans and include potential bioterrorism agents and emerging infectious disease threats, such as Venezuelan, Eastern, and Western equine encephalitis viruses and chikungunya virus (CHIKV). Recent outbreaks caused millions of CHIKV infections in south India and on La Reunion and other islands in the Indian Ocean (3, 4). Although past epidemics of CHIKV have been confined to Africa and Asia, increased air travel has produced increased transmission in Europe and the Western hemisphere (4-6). A mutation enhancing the ability of CHIKV to infect and be transmitted by the Aedes albopictus mosquito has also increased the risk for infection due to the continuing global spread of this vector (7-9). There are currently no approved vaccines or antiviral therapies for CHIKV infection.Alphaviruses infect cells by binding to cell surface receptors, internalization by endocytosis, and low-pH-triggered fusion of the viral and endosomal membranes (reviewed in references 1 and 10). Similarly, CHIKV infection has been shown to require endocytic uptake and low pH (11). Fusion and infection by alphaviruses, such as Semliki Forest virus (SFV) and Sindbis virus (SINV), are promoted by cholesterol in the host cell membrane (12-16), and studies of CHIKV suggest that its infection is also promoted by cholesterol (11,17).The alphavirus membrane i...

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Susceptibility of Florida Mosquitoes to Infection with Chikungunya Virus

Cited by 69 publications

References 28 publications

High Level of Vector Competence of Aedes aegypti and Aedes albopictus from Ten American Countries as a Crucial Factor in the Spread of Chikungunya Virus

High Level of Vector Competence of Aedes aegypti and Aedes albopictus from Ten American Countries as a Crucial Factor in the Spread of Chikungunya Virus

Larval Environmental Temperature and the Susceptibility ofAedes albopictusSkuse (Diptera: Culicidae) to Chikungunya Virus

Cross-Inhibition of Chikungunya Virus Fusion and Infection by Alphavirus E1 Domain III Proteins

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