Susceptibility of different life stages of Tenebrio molitor (Coleoptera: Tenebrionidae) to indigenous entomopathogenic fungi

Eski, Ardahan; Gezgin, Muhammed Murat

doi:10.1016/j.jspr.2022.102008

Cited by 8 publications

(6 citation statements)

References 44 publications

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“…Vuill. and the isolates BL23 and BL24 of Metarhizium anisopliae (Metchnikoff) Sorokin provided 100.0% mortality of T. molitor larvae, while other isolates, i.e., BL1 of B. bassiana did not exceed 17.5% mortality (Eski and Murat Gezgin 2022 ). Interestingly, only the methanol A. oleracea extract caused elevated mortality to T. molitor adults.…”

Section: Discussionmentioning

confidence: 97%

“…Tenebrio molitor larvae are reported as difficult to be managed (Kavallieratos et al 2021a , b , 2022c ). In some rare cases, T. molitor larvae exhibit susceptibility to certain compounds or entomopathogenic fungi isolates (Ntalli et al 2021 ; Eski and Murat Gezgin 2022 ). For example, ( E )-2-decenal and 2-undecanone killed 80.0 and 87.8% of T. molitor larvae, respectively, while trans -anethole only 16.7% at the end of the trial (Ntalli et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

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Acmella oleracea extracts as green pesticides against eight arthropods attacking stored products

Kavallieratos,

Spinozzi,

Filintas

et al. 2023

Environ Sci Pollut Res

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Developing sustainable control tools for managing noxious pests attacking stored foodstuffs is a timely research challenge. Acmella oleracea (L.) R. K. Jansen is a crop widely cultivated for its multiple usages on an industrial level. In this study, the extracts prepared with A. oleracea aerial parts were applied on wheat kernels for the management of eight important arthropod pests attacking stored products, i.e., Cryptolestes ferrugineus, Tenebrio molitor, Oryzaephilus surinamensis, Trogoderma granarium, Tribolium castaneum, Tribolium confusum, Alphitobius diaperinus (adults/larvae), and Acarus siro (adults/nymphs). Extraction of A. oleracea was optimized on the base of the yield and content of spilanthol and other N-alkylamides which were analysed by HPLC–DAD-MS. Two concentrations of n-hexane or methanol extracts (500 ppm and 1000 ppm), obtained through Soxhlet extraction, were tested to acquire mortality data on the above-mentioned pests after 4, 8, and 16 h and 1 to 7 days of exposure. Both extracts achieved complete mortality (100.0%) of C. ferrugineus adults. In the case of A. diaperinus adults, mortalities were very low at any concentrations of both extracts. In general, the n-hexane extract was more efficient than methanol extract against almost all species and stages. Considering both extracts, the susceptibility order, from most to least susceptible species/stage, was C. ferrugineus adults > A. diaperinus larvae > C. ferrugineus larvae > T. granarium adults > T. molitor larvae > O. surinamensis adults > O. surinamensis larvae > T. confusum larvae > T. castaneum larvae > A. siro adults > T. molitor adults > A. siro nymphs > T. granarium larvae > T. castaneum adults > T. confusum adults > A. diaperinus adults. Our research provides useful knowledge on the efficacy of N-alkylamides-rich A. oleracea extracts as grain protectants, pointing out the importance of targeting the most susceptible species/ developmental stages.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Acmella oleracea extracts as green pesticides against eight arthropods attacking stored products

Kavallieratos,

Spinozzi,

Filintas

et al. 2023

Environ Sci Pollut Res

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show abstract

“…The pathogenicity and virulence of various microorganisms and their products have been evaluated for biological control of T. molitor. Specially, the efficacy of entomopathogenic microorganisms Beauveria bassiana, Metarhizium anisopliae and Bacillus thuringiensis on different developmental stages of T. molitor has been reported [23]- [26]. However, there is no study on the efficacy of bacterial pigments against T. molitor.…”

Section: Discussionmentioning

confidence: 99%

Insecticidal Activity of Prodigiosin Pigment on Tenebrio molitor (Coleoptera: Tenebrionidae)

Eski

ÖZDEMİR>

2022

Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi

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Microorganisms and their metabolites have the potential to provide a sustainable alternative to chemical insecticides. Serratia marcescens is an entomopathogenic bacterium that produces hydrolytic enzymes and toxins. It also produces a red pigment called prodigiosin, which has a variety of biological properties. In this study, the prodigiosin pigment was extracted from S. marcescens Se9 isolate with acidified ethanol and concentrated using a rotary evaporator. The insecticidal potential on larval and adult stages of Tenebrio molitor was then investigated. It was found that the mortality rate of larvae exposed to the lowest concentration (125 ppm) of crude pigment was 5%, while the mortality rate of larvae exposed to the highest concentration (2000 ppm) was 68%. The LC50 value of the crude pigment for the larval stage was determined to be 924 ppm. On the other hand, even at the highest pigment concentration (2000 ppm), only 30% mortality was observed in adults. The LC50 value of the crude pigment for the adult stage was determined to be 4570 ppm. It was determined that the pigment had a toxic effect on the pest, but the larval stage was more sensitive than the adult stage. The study showed that prodigiosin pigment appears to be a promising biocontrol agent for use against T. molitor.

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“…Soil samples were collected from different locations in Bilecik, Türkiye, during the summer of 2020. After scraping the surface material, the samples were collected from a depth of 15 cm below the surface using a soil corer and placed in an autoclaved glass jar (Eski & Gezgin, 2022). The samples were transferred to the laboratory for isolation of B. thuringiensis.…”

Section: Isolation Of Bacillus Thuringiensis From Soil Samplesmentioning

confidence: 99%

Farklı Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) popülasyonlarının yerel Bacillus thuringiensis suşlarına duyarlılığı

Eski

2023

TED

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Tomato leafminer, Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) is one of the most important tomato pests worldwide and causes 100% product loss if not controlled. Chemical insecticides, which have been overused for many years, have induced resistance in the pests and made it difficult to control their populations in the field. The use of biological agents that express insecticidal proteins, such as Bacillus thuringiensis, is an alternative to conventional insecticides to suppress pest populations. In this study, to recover novel B. thuringiensis strains from soil samples, a survey was conducted in Bilecik province in 2021. Thirteen local B. thuringiensis strains were isolated and the susceptibility of three different field populations (Samsun, İzmir, and Bilecik) of T. absoluta to these strains was evaluated. Bacillus thuringiensis B3 (Bt-B3) strain, which contains lepidopteran-active toxin genes, was more virulent for all T. absoluta populations tested. In addition, Samsun population was more sensitive to the B3 strain than İzmir and Bilecik. The LC50 values of Bt-B3 were determined to be 13.28, 26.06 and 24.24 ppm for Samsun, İzmir and Bilecik populations, respectively. Sequencing of the 16S rRNA gene region confirmed that the isolate was B. thuringiensis, while electron microscopy revealed that the isolate produced bipyramidal, cubic and spherical insecticidal proteins. The results of this study indicate that the isolate Bt-B3 appears to be a promising biocontrol agent for integrated pest management of T. absoluta in Türkiye.

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Susceptibility of different life stages of Tenebrio molitor (Coleoptera: Tenebrionidae) to indigenous entomopathogenic fungi

Cited by 8 publications

References 44 publications

Acmella oleracea extracts as green pesticides against eight arthropods attacking stored products

Acmella oleracea extracts as green pesticides against eight arthropods attacking stored products

Insecticidal Activity of Prodigiosin Pigment on Tenebrio molitor (Coleoptera: Tenebrionidae)

Farklı Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) popülasyonlarının yerel Bacillus thuringiensis suşlarına duyarlılığı

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