Susceptibility of different chicken lines to H7N1 highly pathogenic avian influenza virus and the role of Mx gene polymorphism coding amino acid position 631

Sironi, Laura; Williams, J. L.; Martin, Ana Moreno; Ramelli, P.; Stella, Alessandra; Han, Jin‐Hee; Weigend, Steffen; Lombardi, Giovanna; Cordioli, Paolo; Mariani, Paola

doi:10.1016/j.virol.2008.07.022

Cited by 52 publications

(49 citation statements)

References 24 publications

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“…In a previous study, Sironi et al (2008) initially observed discrepancies in genotyping data obtained from sequencing and PCR-RFLP approaches. That was the result of an A/G single nucleotide polymorphism in the F primer used in PCR-RFLP.…”

Section: Resultsmentioning

confidence: 99%

“…In a previous study, 85 chickens from three commercial broiler lines and two experimental lines (New Hampshire and White Leghorn) were genotyped with both the PCR-RFLP protocol described here and sequencing. Full identity of results between the two methods was obtained for each of the 85 chickens (Sironi et al, 2008). Forty-two broilers from six other commercial broiler lines were spe- cifically genotyped for the present paper.…”

Section: Resultsmentioning

confidence: 99%

“…DNA was obtained from one hundred and twenty-seven chickens (commercial broilers, White Leghorn and New Hampshire), which was used for genotyping both the PCR-RFLP protocol and sequencing. Some of these data have been published previously by Sironi et al (2008).…”

Section: Methodsmentioning

confidence: 91%

“…Z23168), resulting in the substitution of serine with asparagine at position 631 of the Mx protein, seems to influence the antiviral activity of the molecule (Ko et al, 2002). However, no association was found between the Mx genotype for the G/A polymorphism affecting amino acid position 631 and the survival of chicken experimentally infected in vivo with a highly pathogenic H7N1 avian influenza virus (Sironi et al, 2008). Similar results were obtained by Benfield et al (2008) in their study showing that the Asn631 allele of chicken Mx is unable to inhibit in vitro replication of 5 influenza strains.…”

Section: Introductionmentioning

confidence: 96%

“…In a previous study (Sironi et al, 2008), Mx genotyping for the 2032 polymorphism was carried out by both direct sequencing and PCR-RFLP analysis to compare the genotypes obtained. Some discrepancies were initially observed in the genotype data obtained with the two methods, due to an A/G single nucleotide polymorphism in the sequence of the F primer used for the PCR-RFLP, leading to the preferential amplification of one of the two alleles.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

PCR-RFLP genotyping protocol for chicken Mx gene G/A polymorphism associated with the S631N mutation

Sironi¹,

Ramelli²,

Williams³

et al. 2010

Genet. Mol. Res.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

PCR-RFLP genotyping protocol for chicken Mx gene G/A polymorphism associated with the S631N mutation

Sironi¹,

Ramelli²,

Williams³

et al. 2010

Genet. Mol. Res.

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Association of Mx1 Asn631 variant alleles with reductions in morbidity, early mortality, viral shedding, and cytokine responses in chickens infected with a highly pathogenic avian influenza virus

et al. 2011

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Myxovirus-resistance (Mx) proteins are produced by host cells in response to type I interferons, and some members of the Mx gene family in mammals have been shown to limit replication of influenza and other viruses. According to an early report, chicken Mx1 variants encoding Asn at position 631 have antiviral activity, whereas variants with Ser at 631 lack activity in experiments evaluating Mx1 complementary DNA (cDNA) expressed ectopically in a cell line. We evaluated whether the Mx1 631 dimorphism influenced pathogenesis of highly pathogenic avian influenza virus (HPAIV) infection in chickens of two commercial broiler lines, each segregating for Asn631 and Ser631 variants. Following intranasal infection with HPAIV strain A/Chicken/Queretaro/14588-19/1995 H5N2, chickens homozygous for Asn631 allele were significantly more resistant to disease based on early mortality, morbidity, or virus shedding than Ser631 homozygotes. Higher amounts of splenic cytokine transcripts were observed in the Ser631 birds after infection, consistent with higher viral loads seen in this group and perhaps contributing to their higher morbidity. Nucleotide sequence determination of Mx1 cDNAs demonstrated that the Asn631 variants in the two chicken lines differed at several amino acid positions outside 631. In vitro experiments with a different influenza strain (low pathogenicity) failed to demonstrate an effect of Mx1 Asn631 on viral replication suggesting that in vivo responses may differ markedly from in vitro, or that choice of virus strain may be critical in demonstrating effects of chicken Mx1. Overall, these studies provide the first evidence that Mx1 has antiviral effects in chickens infected with influenza virus.

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A single nucleotide polymorphism of porcine MX2 gene provides antiviral activity against vesicular stomatitis virus

Sasaki

Tungtrakoolsub

Morozumi³

et al. 2013

Immunogenetics

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The objective was to determine if single nucleotide polymorphisms (SNPs) in porcine MX2 gene affect its antiviral potential. MX proteins are known to suppress the multiplication of several viruses, including influenza virus and vesicular stomatitis virus (VSV). In domestic animals possessing highly polymorphic genome, our previous research indicated that a specific SNP in chicken Mx gene was responsible for its antiviral function. However, there still has been no information about SNPs in porcine MX2 gene. In this study, we first conducted polymorphism analysis in 17 pigs of MX2 gene derived from seven breeds. Consequently, a total of 30 SNPs, of which 11 were deduced to cause amino acid variations, were detected, suggesting that the porcine MX2 is very polymorphic. Next, we classified MX2 into eight alleles (A1-A8) and subsequently carried out infectious experiments with recombinant VSVΔG*-G to each allele. In A1-A5 and A8, position 514 amino acid (514 aa) of MX2 was glycine (Gly), which did not inhibit VSV multiplication, whereas in A6 and A7, 514 aa was arginine (Arg), which exhibited the antiviral ability against VSV. These results demonstrate that a SNP at 514 aa (Gly-Arg) of porcine MX2 plays a pivotal role in the antiviral activity as well as that at 631 aa of chicken Mx.

show abstract

Susceptibility of different chicken lines to H7N1 highly pathogenic avian influenza virus and the role of Mx gene polymorphism coding amino acid position 631

Cited by 52 publications

References 24 publications

PCR-RFLP genotyping protocol for chicken Mx gene G/A polymorphism associated with the S631N mutation

PCR-RFLP genotyping protocol for chicken Mx gene G/A polymorphism associated with the S631N mutation

Association of Mx1 Asn631 variant alleles with reductions in morbidity, early mortality, viral shedding, and cytokine responses in chickens infected with a highly pathogenic avian influenza virus

A single nucleotide polymorphism of porcine MX2 gene provides antiviral activity against vesicular stomatitis virus

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