Susceptibility of different cell lines to infection with bovine respiratory syncytial virus

Spilki, Fernando Rosado; Almeida, Renata Servan de; Campalans, Jacqueline; Arns, Clarice Weis

doi:10.1016/j.jviromet.2005.08.002

Cited by 13 publications

(11 citation statements)

References 25 publications

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“…CRIB cells, a clone of Madin-Darby Bovine Kidney (MDBK) cells resistant to the infection with BVDV (FLORES & DONIS, 1995), which is susceptible to the infection with BRSV (FLORES & DONIS, 1995;SPILKI et al, 2006a) were used throughout. Cells were cultivated in Eagle's minimal essential medium (E-MEM) supplemented with 10% Fetal calf serum (FCS), free of antibiotics, following routine protocols.…”

Section: Methodsmentioning

confidence: 99%

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Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants

Spilki¹,

Almeida²,

Arns³

2010

Cienc. Rural

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Bovine respiratory syncytial virus (BRSV) causes pneumonia in young cattle. Modified-live-virus (MLV) and inactivated vaccines are currently used for the control of clinical effects of BRSV infections in cattle.On the present research, the stimulation of specific anti-BRSV immunoglobulin isotypes was investigated, through the use of different commercially available adjuvants (Water-in-oil emulsion, Quil A, Aluminumhydroxide) in inbred mice (Balb/C and C57BL/6) RESUMO O vírus respiratório sincicial bovino (BRSV) causa pneumonia em bovinos jovens. Vacinas de vírus vivo modificado (MLV) e vacinas inativadas são atualmente utilizadas para o controle dos efeitos clínicos de infecções pelo BRSV em bovinos. No presente trabalho, investigou-se a estimulação dos isotipos de imunoglobulinas específicas anti-BRSV, através da utilização de diferentes adjuvantes disponíveis comercialmente (água em óleo de emulsão, Quil A, hidróxido de alumínio) em camundongos isogênicos (Balb/C e C57BL/6). Anticorpos contra o BRSV foram medidos usando-se um ensaio imunoenzimático (ELISA), e os resultados foram comparados com os níveis de anticorpos induzidos pela imunização de

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Section: Methodsmentioning

confidence: 99%

“…BRSV antibodies were measured using an in-house enzyme-linked immunosorbent assay (ELISA) and the results were compared to the IgG isotypes induced by immunization of mice using live-BRSV-virus. Two mice lineages were used, based on its different levels of BRSV infection resistance (SPILKI et al, 2006a).…”

Section: Introductionmentioning

confidence: 99%

Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants

Spilki¹,

Almeida²,

Arns³

2010

Cienc. Rural

Self Cite

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“…The supernatant from respective infected monolayers were collected and assayed for BoHV-5 detection by virus neutralization assay. All the experiments were performed in duplicate and the infectious virus calculated according to SpearmannKärber method as described before [5] .…”

Section: Virus Infection and Propagationmentioning

confidence: 99%

“…The cross-protection theory is based upon rare occurrence of BoHV-5 associated disease in BoHV-1 endemic area or farms with intense vaccination schedules against BoHV-1 [4] . Otherwise, it has been showed that glycoprotein E is inefficient to produce cross protection between BoHV-1 and BoHV-5 [5] . In fact, the vaccine against BoHV-1 is routinely formulated by infection of MDBK (Madin Darby bovine kidney) cell line grown in Eagle Minimal Medium (MEM) supplemented by foetal bovine serum.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, CRIB cells has been reported to be resistant to BVDV infection in vivo derived from MDBK cells and obtained by selection and cloning of cells surviving infection by high cytolitic BVDV strain [6] . For instance, CER cells have been used frequently to isolate and, also propagate rabies virus [11] and recently, described to support bovine syncytial respiratory virus [5] . Nevertheless, there is no report describing the CER use on BoHV virus infection until now.…”

Section: Introductionmentioning

confidence: 99%

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Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium

Cardoso¹,

Ferrari²,

Luvizotto³

et al. 2007

American J. of Biochemistry and Biotechnology

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Abstract:The detection and growth of bovine herpes virus type 5 (BoHV-5) was evaluated in three different media without foetal bovine serum or animal protein by infecting three different cell lines. The OPTI-PRO, VP-SFM and RPMI 1640 media were supplemented by L-glutamine, antibiotics added by 5µg of insulin-like growth factor (IGF-I) and used to adapt CER, MDBK and CRIB cells in a statistic continuous culture system. The results obtained by CRIB and MDBK cells adaptation showed steady growth after 10 th passages in OPTI-PRO medium, 20 th passages in VP-SFM medium and 30 th passages in RPMI 1640 medium, respectively. The OPTI-PRO and RPMI 1640 media were not able to support CER growth, even supplemented by IGF-1 and present apoptotic cells at 72h post-infection. The CER cells seeded after adaptation by 10 th passages in VP-SFM medium added by 5µg/20ml of IGF-1 growth factor revealed the best virus titres compared to MDBK and CRIB cell lines. It was concluded that CER, MDBK and CRIB cells infected by BoHV-5 serotype could be used for laboratory diagnosis propagated on much safer culture system of high biotechnology advances.

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Selection and characterization of canine, swine and rabbit cell lines resistant to bovine viral diarrhea virus

Dezengrini

Weiblen

Flores

2006

Journal of Virological Methods

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Susceptibility of different cell lines to infection with bovine respiratory syncytial virus

Cited by 13 publications

References 25 publications

Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants

Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants

Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium

Selection and characterization of canine, swine and rabbit cell lines resistant to bovine viral diarrhea virus

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