Susceptibility of boar spermatozoa to heat stress using in vivo and in vitro experimental models

Peña, Santiago T.; Stone, Felicity; Gummow, Bruce; Parker, Albert; Paris, Damien B.B.P.

doi:10.1007/s11250-020-02516-y

Cited by 7 publications

(9 citation statements)

References 44 publications

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“…We found that HS resulted in a significant reduction in sperm motility, APD, VSL, STR, and LIN, indicating the susceptibility of boar sperm to high temperatures. Consistent with our results, heat stress also altered the CASA parameters of sperm in boars [ 6 ] and buffalo bulls [ 15 ]. Unexpectedly, HS only reduced the percentage of viable sperm, but did not harm sperm motility and other motion parameters in rabbits [ 17 ].…”

Section: Discussionsupporting

confidence: 91%

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Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa

Sui

Wang

Liu

et al. 2022

Genes

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Heat stress (HS) commonly causes boar infertility and economic loss in the swine industry. The heat tolerance of boar semen presents obvious differences among individuals. However, whether heat stress affects motion characteristics and the metabolome profile in boar sperm remains unclear. In this study, the kinetic features of sperm from HS and non-HS (NHS) groups were detected by computer-assisted sperm analysis, and metabolomic profiling was performed by liquid chromatography–mass spectrometry. The results showed that heat stress significantly reduced sperm motility, average path distance (APD), straight-line velocity (VSL), straightness (STR), and linearity (LIN) (p < 0.05). A total of 528 and 194 metabolites in sperm were identified in the positive and negative ion modes, respectively. Lipids and lipid-like molecules, and organic acids and derivatives were major metabolic classes in the two modes. Furthermore, we separately identified 163 and 171 differential metabolites in the two modes between HS and NHS groups. Clustering analysis further revealed significant metabolic changes in sperm after heat stress. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that differential metabolites in the two modes were enriched in glycerophospholipid, choline, and alanine, aspartate, and glutamate and lysine metabolism. Taken together, these results demonstrate that heat stress can alter the motion characteristics and metabolomic profiles of boar sperm.

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Section: Discussionsupporting

confidence: 91%

“…Thus, the pre-selection of heat-tolerant boar semen could enhance utilization efficiency in artificial insemination programs. Recently, an in vitro heat stress experimental model for boar semen was established to provide a valuable tool to screen for reliable sperm biomarkers of heat stress [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa

Sui

Wang

Liu

et al. 2022

Genes

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“…In this study, the temperature under HS condition ranged from 29–35 °C, with a daily average of 31.56 ± 1.22 °C and THI of 84.68 ± 1.35 (81.96–87.81). The temperature and THI in the HS condition has been reported to be 30–35 °C and 82–86 [ 22 , 31 ], which were used to decide that the sows were experiencing HS. HS reduces pig growth performance and carcass fat quality, causing significant economic losses in the pig industry [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Dietary Supplementation with Mannose Oligosaccharides on the Body Condition, Lactation Performance and Their Offspring of Heat-Stressed Sows

Ren

Zheng²,

Tang³

et al. 2022

Animals

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The aim of this study was to determine the effects of dietary supplementation with mannose oligosaccharide (MOS) on the condition of the body and the reproductive and lactation performances of sows. Eighty pregnant sows were randomly assigned to four groups with a 2 × 2 factorial design: with or without MOS (1 g/kg) and with or without heat stress (HS) challenge. The temperature in the HS groups (HS and HM group) was controlled at 31.56 ± 1.22 °C, while the temperature in the active cooling (AC) groups (AC and AM group) was controlled at 23.49 ± 0.72 °C. The weight loss of sows in the AC group was significantly lower than that of sows in the HS group (p < 0.01). The weight and backfat thickness loss of sows supplemented with MOS displayed a downward trend. The average birth weight of the litter significantly increased in the HM group (basic diet + MOS) compared with the HS group (p < 0.05). The milk protein of sows significantly decreased under the HS condition at 2 and 12 h after delivery (p < 0.05). However, the milk immunoglobin G (IgG) of sows in the HS group increased significantly compared with that of sows in the HM group (p < 0.05) at 12 and 24 h after delivery. The levels of serum urea nitrogen (UREA) and glucose (GLU) decreased significantly under the HS condition (p < 0.05), while the level of interleukin-6 (IL-6) increased significantly under the HS condition (p < 0.05). Dietary supplementation with MOS also significantly reduced TNF-α under the AC conditions (p < 0.05). In conclusion, HS significantly affected the body condition, lactation performances and their offspring of sows. However, dietary supplementation with 1 g/kg MOS did not result in statistically significant changes.

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“…On the other hand, the supporting role of the scrotum on the normal spermatogenesis by testicular thermoregulation is well known [ 9 ], while any factor affecting testis reflects semen quality. Artificially induced heat shock environmental conditions, as well as ultrasonographical and thermographic techniques, have been used to study boar scrotum function and sperm susceptibility [ 10 , 11 ]. Considering that different assays must be applied to enrich semen assessing and advance the prognosis of boar field fertility, the aim of this feasibility study was to record scrotal function and correlate the sperm variables with the frequency/intensity of scrotal contractions (SC) throughout the semen collection.…”

Section: Introductionmentioning

confidence: 99%

Investigating Visual Monitoring of the Scrotum as a Supplementary Tool for Boar Semen Quality Evaluation

Stravogianni

Samaras

Boscos

et al. 2022

Veterinary Sciences

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Farm animals behavior research uses video cameras, mainly for visual observation and recording. The purpose of this feasibility study was to enrich the predictable methods of boar semen production capacity by correlating sperm variables with the scrotal contractions (SC) frequency and intensity. A video camera was used to record the reaction of the scrotum during ejaculation. The respective collected ejaculates were evaluated and semen parameters, such as viability, morphology, membranes functional integrity and kinematics, were determined. The camera recorded the scrotal contractions/relaxations and the video was handled by the Image Processing Toolbox of Matlab (Mathworks Inc., Natick, MA, USA). The SC intensity was verified as a percentage change in the scrotum size among the video frames of maximum contraction and relaxation. The archived data from the frames were analyzed statistically, using a linear mixed effects model that involved sperm assessed parameters. Correlations of the SC intensity with the average path velocity, VAP (R2 = 0.591, p = 0.043) and with the percentage of the cytoplasmic droplets (R2 = 0.509, p = 0.036) were noticed. Previous studies reported the positive correlation of VAP with the number of live-born piglets. In conclusion, video monitoring of the boar scrotal function during ejaculation is useful, but more research is needed to establish its appropriateness as a supplementary method for the prognosis of boar ability to produce high-quality semen.

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Susceptibility of boar spermatozoa to heat stress using in vivo and in vitro experimental models

Cited by 7 publications

References 44 publications

Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa

Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa

Effect of Dietary Supplementation with Mannose Oligosaccharides on the Body Condition, Lactation Performance and Their Offspring of Heat-Stressed Sows

Investigating Visual Monitoring of the Scrotum as a Supplementary Tool for Boar Semen Quality Evaluation

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