Susceptibility of Biomphalaria spp. to infection with Schistosoma mansoni in sympatric and allopatric combinations with observations on the genetic variability between snails

The Susceptibility of <i>Biomphalaria</i> spp. from Mwea Irrigation Scheme in Kenya against <i>Schistosoma mansoni</i> Miracidia Infection

“…In 10 ml beakers, individual snails were exposed to 8 -10 newly hatched miracidia in direct sunlight for 3 -4 hours. The miracidia exposed snails were kept under laboratory condition at room temperature as previously described by [19][20][21].…”

“…The exposed snails were maintained in aquaria each with 48 snails replicated 10 times in a Complete Randomized Design experimental design. The snails were kept under laboratory condition at room temperature as was illustrated by [19][20][21].…”

New scope on the relationship between rotifers and Biomphalaria alexandrina snails

“…Seven weeks post-infection, eggs obtained from the intestines and livers of infected mice were exposed to direct sunlight for approximately 30 min to stimulate miracidial hatching. Each snail was individually exposed to [5][6] active, vigorously swarming S. mansoni miracidia in 5 mL of DTW for 2 h, a period sufficient for maximal miracidial penetration [25] .…”

“…The infectivity powers of cercariae shed from all exposed snails subgroups (SG II-A,-B,-C) were investigated. A total fo 120 locally bred Swiss albino mice, [4][5][6] weeks old and weighing [20][21][22][23][24][25] g, were infected with 80 S. mansoni cercariae per mouse using the paddling technique [24] . Mice were equally distributed among 4 subsequent subgroups (n=40/subgroup), to be subsequently examined along the 1-4 weeks duration of shedding.…”

Variation spatiale et temporelle de la susceptibilité de Biomphalaria pfeifferi, hôte intermédiaire de <i>Schistosoma mansoni</i>, Man, ouest Côte d’Ivoire

“…Pour ce faire, des souris blanches (Mus musculus), utilisées comme hôtes définitifs, ont été infestées avec les cercaires (150 cercaires par souris). Puis, environ 2 mois après, de jeunes B. pfeifferi ont été infestés avec les miracidiums obtenus à partir d'oeufs du schistosome extraits du foie des souris infestées (Freire et al, 2003 ;Mostafa et El-Dafrawy, 2011). (Figure 1).…”