Susceptibility of apple genotypes from European genetic resources to fire blight (Erwinia amylovora)

Sobiczewski, P.; Peil, Andreas; Mikiciński, Artur; Richter, Klaus; Lewandowski, M.; Žurawicz, E.; Kellerhals, M.

doi:10.1007/s10658-014-0521-7

Cited by 26 publications

(36 citation statements)

References 23 publications

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“…The cultivars express different degree of resistance to fire blight (Sobiczewski et al 2015). In relation to leaf age, the constitutive levels of the phenolic compounds were determined in intact plants of both cultivars.…”

Section: Introductionmentioning

confidence: 99%

Phenolic profiles in apple leaves and the efficacy of selected phenols against fire blight (Erwinia amylovora)

et al. 2017

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The content and type of phenolic compounds in apple leaves as potential markers of resistance to fire blight were analysed. The amounts of phenolic acids and flavonoids were determined before and after E. amylovora inoculation of leaves of two cultivars: 'Enterprise' (highly resistant) and 'Idared' (highly susceptible). The basic levels of phenolics in both cultivars was similar but, following the inoculation, in the resistant one faster and more distinguishable changes were observed. The difference between the cultivars was related to the content of the compounds and the rate of release of free phenols from their glucosides. Regarding age dependency, the levels of eight out of 15 phenolics was significantly higher in young leaves of 'Idaredt han in 'Enterprise'. In the older leaves the differences were limited to four compounds. The amount of salicylic acid in 'Idared' was lower than in 'Enterprise'. In 'Idared' accumulation of salicylate after infection was better pronounced than in 'Enterprise'. Higher levels of naringenin glucosides, 4-hydroxbenzoic acid and gentisic acid were found in 'Enterprise'. The activity of 13 phenolics tested in vitro against the pathogen showed that gallic acid, phloroglucinol, hydroquinone and phloretin, suppressed its growth. The aqueous solutions of gallic acid, phloroglucinol and hydroquinone also significantly limited the development of disease on pear fruitlet slices but only hydroquinone maintained its protective activity for longer time. It also showed very high efficacy in preventing disease spread on apple shoots. The study adds novel information on the contribution of specific phenolics to apple resistance to fire blight.

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Section: Introductionmentioning

confidence: 99%

Phenolic profiles in apple leaves and the efficacy of selected phenols against fire blight (Erwinia amylovora)

et al. 2017

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“…Therefore, as repeated inoculation was required, these methods were not applied in further inoculations. In the case of the first method, which has been found to be the best inoculation method, e.g., to test the susceptibility of apple genotypes to fire blight (Sobiczewski et al 2015) or to compare the virulence of E. amylovora strains (Ismail et al 2014), no RNA was obtained 24 h after inoculation (data not shown) because of very low bacterial concentrations.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of different RNA extraction methods for high-quality total RNA and mRNA from Erwinia amylovora in planta

et al. 2016

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Obtaining a sufficient quantity of high-quality, intact RNA is the first crucial step in its study by RNA sequencing on next-generation sequencing platforms or quantitative PCR. Different RNA extraction methods or commercial kits vary in yield and in the quality and integrity of the RNA obtained, which may affect the results of downstream applications. Often, these factors depend on the organism under study and nature of the sample. Therefore, the selection of an appropriate RNA isolation method is critical. In this study, we present the results of an evaluation of three different commercial kits for the isolation of total RNA from Erwinia amylovora in apple tissue as well as the usefulness of different kinds of Deoxyribonuclease I for DNA removal and kits for rRNA depletion. To our knowledge, this is the first report on the method of isolation of high-quality E.amylovora mRNA for RNA-seq.

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“…The considerable FB resistance of ‘Hesztia’ (Sobiczewski et al. , , Tóth et al. ) suggests the additive effect of these resistance alleles and a potential strategy of pyramiding resistance genes in apple breeding programmes.…”

Section: Discussionmentioning

confidence: 99%

Identification of marker alleles linked to fire blight resistance QTLs in apple genotypes

et al. 2015

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Molecular marker analysis can be an effective tool when searching for new fire blight resistance donors. It can speed up the breeding process as well, even though many of the available markers linked to fire blight resistance QTLs have not yet been tested by screening a large number of cultivars. The aim of this study was to search for alternate sources of the three major QTLs of fire blight resistance; FBF7, FB_MR5 and FB_E, as well as to test the efficiency of some markers linked to minor QTLs. Altogether, nine primer pairs were used on 77 genotypes including new Hungarian cultivars and old apple cultivars from the Carpathian basin. Several marker alleles of FB resistance QTLs have been detected in the screened genotypes, most importantly the alleles coupling with FB_MR5 in the old cultivars ‘Kéresi muskotály’, ‘Szabadkai szercsika’ and ‘Batul’. We propose these cultivars as the first available resistance donors of FB_MR5 instead of the crabapple Malus × robusta 5. The results also bring new information regarding the resistance alleles of new Hungarian cultivars and selections.

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Susceptibility of apple genotypes from European genetic resources to fire blight (Erwinia amylovora)

Cited by 26 publications

References 23 publications

Phenolic profiles in apple leaves and the efficacy of selected phenols against fire blight (Erwinia amylovora)

Phenolic profiles in apple leaves and the efficacy of selected phenols against fire blight (Erwinia amylovora)

Evaluation of different RNA extraction methods for high-quality total RNA and mRNA from Erwinia amylovora in planta

Identification of marker alleles linked to fire blight resistance QTLs in apple genotypes

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