Susceptibility of Aminoglycoside Resistant  Acinetobacter baumannii  to Antibiotic Combinations

Safe, Feriala A. Abo; Salah, N. Ben; Rashed, Mohamed E.; Saad, Sarah

doi:10.21608/ejm.2019.16809.1115

Cited by 2 publications

(3 citation statements)

References 36 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A study in China also found total resistance to cefazolin and 71.8% to cefuroxime ( 34 ). High resistance percentages to cefoperazone (61%), cefixime (99%), and cefuroxime (96%) were also reported from 250 bacterial isolates in Egypt ( 35 ). A high resistance percentage in the cephalosporin class may occur due to A. baumannii having an AmpC cephalosporinase-type enzyme that is chromosomally encoded by all A. baumannii strains ( 36 ).…”

Section: Discussionmentioning

confidence: 93%

Early detection of a possible multidrug-resistant Acinetobacter baumannii outbreak in the local hospital setting by using random amplified polymorphism DNA-polymerase chain reaction (RAPD-PCR), oxacillinase gene profiles, and antibiograms

Fatmawati,

Suwardana,

Wahyudevi Dharmika

et al. 2023

IJM

View full text Add to dashboard Cite

Background and Objectives: Detecting the source of a potential outbreak of multidrug resistant (MDR) Acinetobacter bau- mannii is necessary to be investigated. This study aimed to detect the possibility of A. baumannii outbreak in a hospital set- ting using a combination of random amplified polymorphism DNA- polymerase chain reaction (RAPD-PCR), antibiograms, and the presence of oxacillinase genes. Materials and Methods: The antibiogram of 31 clinical isolates and six environmental isolates of A. baumannii were de- termined by Vitek® 2 Compact. Oxacillinase genes (OXA-23, -24, -51, and -58) were detected by PCR, and RAPD-PCR was conducted using DAF-4 and ERIC-2 primers. The Similarity Index and dendrogram were generated using GelJ v2.3 software. Results: The antibiograms showed that all MDR A. baumannii isolates has very limited susceptibility to cephalosporins, but mostly susceptible to tigecycline. All isolates were positive for bla gene, thirty-two of 37 total isolates (86.5%) were positive for bla OXA-23-like gene, and none were positive for bla OXA-24-like OXA-58-like genes. RAPD-PCR showed that the DAF-4 primer on average had more band visualization and lower Similarity Index’s variation compared to the ERIC-2. The discriminatory power of DAF-4 was 0.906. There was a significant correlation between the DAF-4 dendrogram pattern with the antibiogram (r=0.494, p<0.001) and the presence of bla gene (r=0.634, p<0.001) from all ICU A isolates. Six out of fourteen ICU A isolates belonged to the same cluster with >95% Similarity Index, while one clinical isolate having an identical dendrogram and antibiogram pattern with an environmental isolate within this cluster. Conclusion: There is a high probability of MDR A. baumannii outbreak within ICU A detected by multiple analysis of RAPD-PCR, antibiogram and the bla OXA-23-like gene profiles. This combinatorial approach is conceivable to mitigate possible outbreak situations of A. baumannii in the local hospital without sophisticated microbiology laboratory.

show abstract

Section: Discussionmentioning

confidence: 93%

Early detection of a possible multidrug-resistant Acinetobacter baumannii outbreak in the local hospital setting by using random amplified polymorphism DNA-polymerase chain reaction (RAPD-PCR), oxacillinase gene profiles, and antibiograms

Fatmawati,

Suwardana,

Wahyudevi Dharmika

et al. 2023

IJM

View full text Add to dashboard Cite

show abstract

“…MRSA and A. baumannii are the most common pathogens causing HAIs in Egypt [ 41 , 75 ]. Therefore, time–kill curves and TEM images for treated bacteria were performed only for those bacteria.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the current study is considered the first assessment of the antibacterial activity and safety of ZnO-NPs compared to a commercial alcohol-based hand sanitizer (Sterillium) that is commonly used in Egyptian hospitals and communities [ 38 ]. In the current study, ZnO-NPs were synthesized chemically, and the antibacterial activity of their colloidal solution in comparison to Sterillium was investigated against multidrug-resistant bacteria that are commonly known to cause HAIs in Egypt: Acinetobacter baumannii (A. baumannii), Klebsiella pneumoniae (K. pneumoniae), Methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus (S. aureus) [ 39 , 40 , 41 ]. The cytotoxicity of these formulations to human dermal fibroblast cells was also assessed to ascertain the biosafety (in vitro) of ZnO-NPs to replace alcohol-based hand sanitizers in healthcare settings.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Antibacterial Activity and Safety of Zinc Oxide Nanoparticles versus a Commercial Alcohol-Based Hand-Sanitizer: Can Zinc Oxide Nanoparticles Be Useful for Hand Sanitation?

et al. 2022

View full text Add to dashboard Cite

Hand hygiene is the key factor to control and prevent the spread of infections, for example, hospital-acquired infections (HAIs). People commonly use alcohol-based hand sanitizers to assure hand hygiene. However, frequent use of alcohol-based hand sanitizers in a pandemic situation (e.g., COVID-19) was associated with serious drawbacks such as skin toxicity including irritation, skin dermatitis, and skin dryness or cracking, along with peeling, redness, or itching with higher possibility of infection. This demands the development of alternative novel products that are effective as alcohol-based hand sanitizers but have no hazardous effects. Zinc oxide nanoparticles (ZnO-NPs) are known to have broad-spectrum antimicrobial activity, be compatible with the biological system and the environment, and have applicable and economic industrial-scale production. Thus, ZnO-NPs might be a good candidate for hand sanitation. To the best of our knowledge, the antibacterial activity of ZnO-NPs in comparison to alcohol-based hand sanitizers has not yet been studied. In the present work, a comparative study of the antibacterial activity of ZnO-NPs vs. Sterillium, a commercial alcohol-based hand sanitizer that is commonly used in Egyptian hospitals, was performed against common microorganisms known to cause HAIs in Egypt, including Acinetobacter baumannii, Klebsiella pneumoniae, Methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus. The safety profiles of ZnO-NPs and Sterillium were also assessed. The obtained results demonstrated the superior antibacterial activity and safety of ZnO-NPs compared to Sterillium. Therefore, ZnO-NPs could be a promising candidate for hand sanitation in comparison to alcohol-based hand sanitizers; however, several studies related to long-term toxicity and stability of ZnO-NPs and investigations into their antimicrobial activity and safety in healthcare settings are still required in the future to ascertain their antimicrobial activity and safety.

show abstract

Susceptibility of Aminoglycoside Resistant Acinetobacter baumannii to Antibiotic Combinations

Cited by 2 publications

References 36 publications

Early detection of a possible multidrug-resistant Acinetobacter baumannii outbreak in the local hospital setting by using random amplified polymorphism DNA-polymerase chain reaction (RAPD-PCR), oxacillinase gene profiles, and antibiograms

Early detection of a possible multidrug-resistant Acinetobacter baumannii outbreak in the local hospital setting by using random amplified polymorphism DNA-polymerase chain reaction (RAPD-PCR), oxacillinase gene profiles, and antibiograms

Investigating the Antibacterial Activity and Safety of Zinc Oxide Nanoparticles versus a Commercial Alcohol-Based Hand-Sanitizer: Can Zinc Oxide Nanoparticles Be Useful for Hand Sanitation?

Contact Info

Product

Resources

About