Susceptibility and Selectivity of &lt;I&gt;Cnaphalocrocis medinalis&lt;/I&gt; (Lepidoptera: Pyralidae) to Different Cry Toxins

Yang, Yajun; Xu, Hongwei; Zheng, Xiaodong; Lü, Zhongxian

doi:10.1603/ec12236

Cited by 12 publications

(14 citation statements)

References 52 publications

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“…; Yang et al . ). However, there are only few reports on the toxicity of Cry1Da despite the fact that it is active against the polyphagous Spodoptera insects.…”

Section: Introductionmentioning

confidence: 97%

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Enhanced Cry1Da production inBacillus thuringiensisby driving expression from the σ^E-dependent BtI promoter

2013

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Aims To increase the Cry1Da production in Bacillus thuringiensis by enhancing BtI promoter activity and fusion with upstream sequence from cry1Ab. Methods and Results The effects of joining the upstream sequence of cry1Ab that contains E2 subunit pyruvate dehydrogenase (PDH) recognition site to the cry1Da promoter as well as the effects of substitution mutation of conserved sequences of its BtI promoter on cry1Da expression was monitored by constructing cry1Da promoter‐lacZ fusions. Changing the −35 region of the cry1Da BtI promoter to that of cry1Ab enhanced β‐galactosidase activity about three fold as comparing to that of the wild‐type promoter with its own upstream sequence. In contrast, the same cry1Da mutated promoter linked to the above upstream sequence of cry1Ab enhanced enzyme activity up to seven fold, but was five fold lower than that of the full‐length cry1Ab promoter. The cry1Ab‐cry1Da hybrid promoter with the −35 BtI mutation efficiently increased Cry1Da synthesis by 133% and resulted in a 2·3‐fold increase in insect larval toxicity when comparing to the wild type. Conclusions The cry1Ab promoter as well as mutation of −35 region of BtI promoter together with fusion with E2 subunit PDH recognition site efficiently enhanced Cry1Da production in B. thuringiensis. Significance and Impact of the study The results provide useful information to construct an efficient cry1Da gene expression in B. thuringiensis.

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“…; Yang et al . ). However, there are only few reports on the toxicity of Cry1Da despite the fact that it is active against the polyphagous Spodoptera insects.…”

Section: Introductionmentioning

confidence: 97%

“…There are many reports on testing different Cry toxins such as Cry1Aa, Cry1Ab and Cry1C against many insect larvae (Xue et al 2005;Yang et al 2012). However, there are only few reports on the toxicity of Cry1Da despite the fact that it is active against the polyphagous Spodoptera insects.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Cry1Da production inBacillus thuringiensisby driving expression from the σ^E-dependent BtI promoter

2013

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“…Environment-friendly methods are recommended in the pest control. Bacillus thuringiensis , as one of the important biological agents has been applied in the pest control for more than 60 years due to its crystal protein with insecticidal activity against insect pests [ 7 – 9 ]. Cry genes derived from B .…”

Section: Introductionmentioning

confidence: 99%

Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin

Yang

et al. 2018

PLoS ONE

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Cnaphalocrocis medinalis (Guenée) is one of the important insect pests in rice field. Bt agents were recommended in the C. medinalis control and Bt rice is bred as a tactic to control this insect. However, the tolerance or resistance of insect to Bt protein is a main threat to the application of Bt protein. In order to investigate the response of C. medinalis transcriptome in defending a Cry1C toxin, high-through RNA-sequencing was carried in the C. medinalis larvae treated with and without Cry1C toxin. A total of 35,586 high-quality unigenes was annotated in the transcriptome of C. medinalis midgut. The comparative analysis identified 6,966 differently expressed unigenes (DEGs) between the two treatments. GO analysis showed that these genes involved in proteolysis and extracellular region. Among these DEGs, carboxylesterase, glutathione S-transferase and P450 were differently expressed in the treated C. medinalis midgut. Furthermore, trypsin, chymotrypsin, and carboxypeptidase were identified in DEGs, and most of them up-regulated. In addition, thirteen ABC transporters were downregulated and three upregulated in Cry1C-treated C. medinalis midgut. Based on the pathway analysis, antigen processing and presentation pathway, and chronic myeloid leukemia pathway were significant in C. medinalis treated with Cry1C toxin. These results indicated that serine protease, detoxification enzymes and ABC transporter, antigen processing and presentation pathway, and chronic myeloid leukemia pathway may involved in the response of C. medinalis to Cry1C toxin. This study provides a transcriptomal foundation for the identification and functional characterization of genes involved in the toxicity of Bt Cry protein against C. medinalis, and provides potential clues to the studies on the tolerance or resistance of an agriculturally important insect pest C. medinalis to Cry1C toxin.

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“…For instance, transgenic broccoli containing both Cry1Ac and Cry1C Bt genes effectively controlled diamondback moths resistant to either single protein (Cao et al, 2002). Cry1Ig is a newly discovered gene which showed high insecticidal activity to lepidopterans (data unpublished), while Cry1Ac is an elite insecticidal Bt gene which was highly insecticidal to striped stem borer and rice leaf roller (Yang et al, 2012), two major rice pests in China. Furthermore, Ruiz de Escudero et al (2006) demonstrated that Cry1I shared different binding sites in midgut membrane vesicles from Cry1Ac, suggesting that cross resistance between Cry1Ac and Cry1Ig was unlikely to occur.…”

Section: Discussionmentioning

confidence: 99%

Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene

Zhao

Liu

Zhang

et al. 2015

J. Zhejiang Univ. Sci. B

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Abstract:Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (G10) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice.

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Susceptibility and Selectivity of <I>Cnaphalocrocis medinalis</I> (Lepidoptera: Pyralidae) to Different Cry Toxins

Cited by 12 publications

References 52 publications

Enhanced Cry1Da production inBacillus thuringiensisby driving expression from the σ^E-dependent BtI promoter

Enhanced Cry1Da production inBacillus thuringiensisby driving expression from the σ^E-dependent BtI promoter

Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin

Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene

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Susceptibility and Selectivity of <I>Cnaphalocrocis medinalis</I> (Lepidoptera: Pyralidae) to Different Cry Toxins

Cited by 12 publications

References 52 publications

Enhanced Cry1Da production inBacillus thuringiensisby driving expression from the σE-dependent BtI promoter

Enhanced Cry1Da production inBacillus thuringiensisby driving expression from the σE-dependent BtI promoter

Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin

Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene

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Enhanced Cry1Da production inBacillus thuringiensisby driving expression from the σ^E-dependent BtI promoter

Enhanced Cry1Da production inBacillus thuringiensisby driving expression from the σ^E-dependent BtI promoter