Susceptibility and resistance of inbred mice to Giardia muris

Belosevic, Miodrag; Faubert, G M; Skamene, Emil; MacLean, J D

doi:10.1128/iai.44.2.282-286.1984

Cited by 35 publications

(35 citation statements)

References 14 publications

(3 reference statements)

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“…The parasite has been maintained in the laboratory since 1981 by biweekly passages in CD-1 or C3H/HeN mice. Methods for mouse inoculation and subsequent cyst collection and puri¢cation from mouse feces were those used previously [8,15]. Puri-¢ed cysts for UV experiments were stored in deionized water and used within 48 h of collection.…”

Section: Parasitesmentioning

confidence: 99%

“…For ex vivo experiments, cohorts of ¢ve adult male C3H/HeN mice were inoculated with between 10 4 and 10 5 UV-exposed cysts. Cyst inactivation was determined using the latent period assay described previously for G. muris [8,15].…”

Section: Infectivity Analysismentioning

confidence: 99%

“…Seven days post inoculation neonate mice were examined for the presence of the parasites in their gastrointestinal tract using procedures described recently [5,16]. Log inactivation of G. muris and C. parvum in both the UV-exposed and control samples was determined using the previously described infectivity models [5,8,15,16] and was reported as a function of the post UV exposure storage period.…”

Section: Experimental Designmentioning

confidence: 99%

“…As the neonates grow and develop, their susceptibility to C. parvum decreases such that by days 10^14 post inoculation neonates invariably eliminate the parasites. This is not the case with the G. muris animal infectivity model, where the parasites multiply and thrive in adult, fully immunocompetent animals, and are eventually eliminated in 4^6 weeks post infection [8,15]. In the in vivo experiments, ¢ve control and ¢ve UVtreated (10, 25, 60 and 240 mJ/cm 2 ) mice were inoculated with either 10 or 100 cysts each, depending on the experiment.…”

Section: Experimental Designmentioning

confidence: 99%

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Studies on the resistance/reactivation of Giardia muris cysts and Cryptosporidium parvum oocysts exposed to medium-pressure ultraviolet radiation

Belosevic¹

2001

FEMS Microbiology Letters

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The ex vivo and in vivo reactivation of Giardia muris cysts and Cryptosporidium parvum oocysts after exposure to different doses of ultraviolet (UV) radiation was determined using animal infectivity. The infectivity of UV-treated parasites stored for 1^4 days (G. muris) or 1^17 days (C. parvum) at room temperature in the dark was similar to that of organisms administered immediately after UV treatment, indicating that the parasites did not reactivate ex vivo. In contrast, we observed in vivo reactivation of G. muris in three of seven independent animal infectivity experiments, when parasites were treated with relatively low doses of medium-pressure UV ( 6 25 mJ/cm 2 ). Our observations indicate that G. muris cysts and C. parvum oocysts exposed to medium-pressure UV doses of 60 mJ/cm 2 or higher did not exhibit resistance to and/or reactivation following treatment. This suggests that when appropriate doses of UV are used, significant and permanent inactivation of these parasites may be achieved. ß

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Section: Parasitesmentioning

confidence: 99%

Section: Infectivity Analysismentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

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Studies on the resistance/reactivation of Giardia muris cysts and Cryptosporidium parvum oocysts exposed to medium-pressure ultraviolet radiation

Belosevic¹

2001

FEMS Microbiology Letters

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“…Upon arrival from the animal breeders, the animals were housed in a specific pathogen-free facility (P-2 level) of Ihe Department of Zoology, University of Alberta, Canada. The protocols and procedures for the maintenance ofa Giardia-fvee animal colony have been described previously [21,22].…”

Section: Parasitementioning

confidence: 99%

Antimicrobial action of antibodies against Giardia muris trophozoites

Belosevic

Faubert

Dharampaul

1994

Clinical and Experimental Immunology

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The activities of immune serum and trophozoite-specific MoAb were examined in vitro and in vivo. Immune serum and anti-Giardia muris MoAb caused immobilization of the trophozoites in vitro and were cytotoxic for trophozoites in the presence of exogenous complement. Both immune serum obtained from experimentally infected mice and anti-G. muris MoAb administered directly into the duodenum of mice significantly reduced the number of trophozoites in the small intestine during the acute phase of the infection. These results suggest that serum antibodies play a central role in the elimination of the primary Giardia infection.

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Genetic susceptibility to respiratory syncytial virus infection in inbred mice

Stark

McDowell

Koenigsknecht

et al. 2002

Journal of Medical Virology

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Differences in the severity of respiratory syncytial virus (RSV)-induced lower respiratory disease in infants have been attributed to multiple environmental and genetic factors. To identify the genetic factor(s) influencing RSV susceptibility, we examined RSV infection in eight inbred mouse strains. Lung RSV titers differed significantly between mouse strains: the RSV titers were 15-fold higher in AKR/J (permissive) mice compared with C57BL/6J (resistant) mice at 4 days after inoculation. This strain-specific difference in RSV titers suggested that susceptibility to RSV infection was attributable to genetic differences between strains. To examine the mode of inheritance of RSV susceptibility, F1 and backcross (F1 x AKR/J) progeny were infected and RSV titers determined. RSV titers in the F1 progeny were similar to those found in the resistant (C57BL/6J) parent, suggesting resistance was inherited as a dominant trait. The distribution of RSV titers in backcross progeny were discordant with that predicted for a single gene effect, suggesting susceptibility was influenced by more than one gene. These data suggest that RSV susceptibility is a multigenic trait that should be amenable to resolution by genomic analysis.

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Susceptibility and resistance of inbred mice to Giardia muris

Cited by 35 publications

References 14 publications

Studies on the resistance/reactivation of Giardia muris cysts and Cryptosporidium parvum oocysts exposed to medium-pressure ultraviolet radiation

Studies on the resistance/reactivation of Giardia muris cysts and Cryptosporidium parvum oocysts exposed to medium-pressure ultraviolet radiation

Antimicrobial action of antibodies against Giardia muris trophozoites

Genetic susceptibility to respiratory syncytial virus infection in inbred mice

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