SUSA2 is an F-box protein required for autoimmunity mediated by paired NLRs SOC3-CHS1 and SOC3-TN2

Liang, Wanwan; Tong, Meixuezi; Li, Xin

doi:10.1038/s41467-020-19033-z

Cited by 23 publications

(13 citation statements)

References 73 publications

(124 reference statements)

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“…Silencing of SGT1/RAR1/HSP90 components alleviates autoimmune responses in several autoimmune mutants. For example, SGT1b is a positive regulator of the autoimmune response displayed by chs3 mutants (Xu et al 2015), HSP90.3 is required for the constitutive activation of defense response in the saul1-1 mutant (Liang et al 2020) and topp4-1 autoimmune phenotypes require RAR1 and HSP90.2 (Yan et al 2019). Similarly, the spontaneous cell death and hyperaccumulation of ROS exhibited by plants overexpressing MSL10-GFP required SGT1b, RAR1 , and HSP90 in both tobacco and Arabidopsis ( Figure 3, S2 and S3 ).…”

Section: Discussionmentioning

confidence: 99%

The Mechanosensitive Ion Channel MSL10 Modulates Susceptibility toPseudomonas syringaeinArabidopsis thaliana

Basu

Codjoe

Veley

et al. 2021

Preprint

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Plants sense and respond to molecular signals associated with the presence of pathogens and their virulence factors. Mechanical signals generated during pathogenic invasion may also be important, but their contributions have rarely been studied. Here we investigate the potential role of a mechanosensitive ion channel, MscS-Like (MSL)10, in defense against the bacterial pathogen Pseudomonas syringae in Arabidopsis thaliana. We previously showed that overexpression of MSL10-GFP, phospho-mimetic versions of MSL10, and the gain-of-function allele msl10-3G all produce dwarfing, spontaneous cell death, and the hyperaccumulation of reactive oxygen species. These phenotypes are shared by many autoimmune mutants and are frequently suppressed by growth at high temperature in those lines. Here, we found that the same was true for all three MSL10 hypermorphs. In addition, we show that the SGT1/RAR1/HSP90 co-chaperone complex was required for dwarfing and ectopic cell death, PAD4 and SID2 were partially required, and the immune regulators EDS1 and NDR1 were dispensable. All MSL10 hypermorphs exhibited reduced susceptibility to infection by P. syringae strain Pto DC3000, Pto DC3000 expressing the avirulence genes avrRpt2 or avrRpm1, but not Pto DC3000 hrpL, and showed an accelerated induction of PR1 expression compared to wild-type plants. Null msl10-1 mutants were delayed in PR1 induction and displayed modest susceptibility to infection by COR-deficient Pst. Finally, stomatal closure was reduced in msl10-1 loss-of-function mutants in response to Pst COR−. These data show that MSL10 modulates pathogen responses and begin to address the possibility that mechanical signals are exploited by the plant for pathogen perception.

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Section: Discussionmentioning

confidence: 99%

The Mechanosensitive Ion Channel MSL10 Modulates Susceptibility toPseudomonas syringaeinArabidopsis thaliana

Basu

Codjoe

Veley

et al. 2021

Preprint

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“…The head-to-head arranged NLR gene pairs have been reported in many studies [ 33 , 38 , 40 , 48 , 49 , 50 , 51 , 52 ]. In this article, we found that there was a large TN - TNL gene cluster on Arabidopsis chromosome 1 and showed the association of TN10 with TNL40TIR and TNL60TIR.…”

Section: Discussionmentioning

confidence: 99%

“…The head-to-head genomic arrangements play important roles in regulating the cooperation of NLR proteins as almost all of the identified paired NLRs are encoded by physically linked genes [ 40 ]. For example, the SOC3-CHS1 pair and SOC3-TN2 pair are the two firstly identified unique NLR pairs formed by a full-length TNL and a truncated TN protein [ 33 , 49 ]. The over-accumulation and disappearance of Arabidopsis E3 ligase SAUL1 are monitored by SOC3-TN2 and SOC3-TN1, respectively [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…The over-accumulation and disappearance of Arabidopsis E3 ligase SAUL1 are monitored by SOC3-TN2 and SOC3-TN1, respectively [ 32 , 33 ]. SOC3 , CHS1 , and TN2 genes also show head-to-head genomic arrangement on chromosome 1 [ 31 , 33 , 49 ]. These reports suggested that the analysis of the interaction of NLR proteins in gene clusters may reveal new NLR protein pairs [ 35 , 38 , 39 , 40 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

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A Truncated TIR-NBS Protein TN10 Pairs with Two Clustered TIR-NBS-LRR Immune Receptors and Contributes to Plant Immunity in Arabidopsis

Chen

Zhong

Cai

et al. 2021

IJMS

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The encoding genes of plant intracellular nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domain receptors (NLRs) often exist in the form of a gene cluster. Several recent studies demonstrated that the truncated Toll/interleukin-1 receptor-NBS (TIR-NBS) proteins play important roles in immunity. In this study, we identified a large TN gene cluster on Arabidopsis ecotype Col-0 chromosome 1, which included nine TN genes, TN4 to TN12. Interestingly, this cluster also contained two typical TIR-NBS-LRR genes: At1g72840 and At1g72860 (hereinafter referred to as TNL40 and TNL60, respectively), which formed head-to-head genomic arrangement with TN4 to TN12. However, the functions of these TN and TNL genes in this cluster are still unknown. Here, we showed that the TIR domains of both TNL40 and TNL60 associated with TN10 specifically. Furthermore, both TNL40TIR and TNL60TIR induced cell death in Nicotiana tabacum leaves. Subcellular localization showed that TNL40 mainly localized in the cytoplasm, whereas TNL60 and TN10 localized in both the cytoplasm and nucleus. Additionally, the expression of TNL40, TNL60, and TN10 were co-regulated after inoculated with bacterial pathogens. Taken together, our study indicates that the truncated TIR-NBS protein TN10 associates with two clustered TNL immune receptors, and may work together in plant disease resistance

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“…tomato strain DC3000 [ 19 ]. The TIR-NB-LRR immune receptor suppressor of CHS1, 2, 3 (SOC3) interacts with CHS1 or TN2 and the SCF complex to regulate innate immunity [ 20 , 21 ]. Recently, the singleton TN, dangerous mix 10 (DM10), was reported to cause severe hybrid necrosis, which is typically induced by a conflict between divergent alleles of immunity genes coming from different parental accessions [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

The Capsicum baccatum-Specific Truncated NLR Protein CbCN Enhances the Innate Immunity against Colletotrichum acutatum

Son

Kim

Lee

et al. 2021

IJMS

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Chili pepper (Capsicum annuum) is an important fruit and spice used globally, but its yield is seriously threatened by anthracnose. Capsicum baccatum (C. baccatum) is particularly valuable as it carries advantageous disease resistance genes. However, most of the genes remain to be identified. In this study, we identified the C. baccatum-specific gene CbCN, which encodes a truncated nucleotide-binding and leucine-rich repeat protein in the anthracnose resistant chili pepper variety PBC80. The transcription of CbCN was greater in PBC80 than it was in the susceptible variety An-S after Colletotrichum acutatum inoculation. In order to investigate the biological function of CbCN, we generated transgenic tobacco lines constitutively expressing CbCN. Notably, CbCN-overexpressing transgenic plants exhibited enhanced resistance to C. acutatum compared to wild-type plants. Moreover, the expression of pathogenesis-related (PR) genes was remarkably increased in a CbCN-overexpressing tobacco plants. In order to confirm these results in chili pepper, we silenced the CbCN gene using the virus-induced gene silencing system. The anthracnose resistance and expressions of PR1, PR2, and NPR1 were significantly reduced in CbCN-silenced chili peppers after C. acutatum inoculations. These results indicate that CbCN enhances the innate immunity against anthracnose caused by C. acutatum by regulating defense response genes.

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SUSA2 is an F-box protein required for autoimmunity mediated by paired NLRs SOC3-CHS1 and SOC3-TN2

Cited by 23 publications

References 73 publications

The Mechanosensitive Ion Channel MSL10 Modulates Susceptibility toPseudomonas syringaeinArabidopsis thaliana

The Mechanosensitive Ion Channel MSL10 Modulates Susceptibility toPseudomonas syringaeinArabidopsis thaliana

A Truncated TIR-NBS Protein TN10 Pairs with Two Clustered TIR-NBS-LRR Immune Receptors and Contributes to Plant Immunity in Arabidopsis

The Capsicum baccatum-Specific Truncated NLR Protein CbCN Enhances the Innate Immunity against Colletotrichum acutatum

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