2011
DOI: 10.1093/nar/gkr496
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SUS1 introns are required for efficient mRNA nuclear export in yeast

Abstract: Efficient coupling between mRNA synthesis and export is essential for gene expression. Sus1/ENY2, a component of the SAGA and TREX-2 complexes, is involved in both transcription and mRNA export. While most yeast genes lack introns, we previously reported that yeast SUS1 bears two. Here we show that this feature is evolutionarily conserved and critical for Sus1 function. We determine that while SUS1 splicing is inefficient, it responds to cellular conditions, and intronic mutations either promoting or blocking … Show more

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Cited by 28 publications
(44 citation statements)
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References 44 publications
(62 reference statements)
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“…5) suggests that the reduced amount of Sus1 protein likely produced by these mutants is still enough to partially complement Sus1 functions, including sus1Δ temperature sensitive growth defect (data not shown). This is interesting, since overexpression of Sus1 has been shown to largely impair its functionality (Cuenca-Bono et al 2011;Hossain et al 2011). …”
Section: Discussionmentioning
confidence: 99%
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“…5) suggests that the reduced amount of Sus1 protein likely produced by these mutants is still enough to partially complement Sus1 functions, including sus1Δ temperature sensitive growth defect (data not shown). This is interesting, since overexpression of Sus1 has been shown to largely impair its functionality (Cuenca-Bono et al 2011;Hossain et al 2011). …”
Section: Discussionmentioning
confidence: 99%
“…Fluorescent in situ hybridization (FISH) against poly(A) + RNA was done as described by Cuenca-Bono et al (2011); yeast cells were grown in 100 mL of SC-Leu medium at 30°C to an 0.3 OD600. Then, cultures were rapidly shifted to 37°C incubator for 2 h. After hybridization, slides were mounted using VECTASHIELD Mounting Medium with DAPI.…”
Section: In Situ Hybridization (Fish)mentioning
confidence: 99%
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“…cerevisiae genes are intronless, the SUS1 gene consists of three exons (of 71, 140 and 77 nt) and two introns (of 80 and 70 nt), and its splicing and expression levels are regulated by different mechanisms [1], [52]. [53], [54].…”
Section: Sus1: An Uncommon Two-intron Yeast Genementioning
confidence: 99%