2015
DOI: 10.1007/s00253-015-7067-2
|View full text |Cite
|
Sign up to set email alerts
|

Surviving the acid barrier: responses of pathogenic Vibrio cholerae to simulated gastric fluid

Abstract: When bacteria are subjected to low acidic pHs of the gastric environment, they may enter the viable but nonculturable (VBNC) state of survival. In this state, bacteria cannot be cultured on solid media, still exhibit signs of metabolic activity (viability). In this study, the response of pathogenic Vibrio cholerae O1 and O139 to low pH-simulated environments of the human stomach was evaluated for their survival by culturability (plate count) and viability (flow cytometry-FC) assays. Bacteria were acid challeng… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

1
25
0

Year Published

2018
2018
2023
2023

Publication Types

Select...
6

Relationship

1
5

Authors

Journals

citations
Cited by 17 publications
(26 citation statements)
references
References 42 publications
1
25
0
Order By: Relevance
“…These bacteria were grown on culturable agar specific for Vibrio sp. to obtain pure colonies as described previously by Singh and Barnard [ 1 ]. From the pure colonies, the V. cholerae were then grown in nutrient broth (pH 7) (BioLab) to the late stationary phase to an optical density of ~0.9 OD 600nm .…”
Section: Methodsmentioning
confidence: 99%
See 4 more Smart Citations
“…These bacteria were grown on culturable agar specific for Vibrio sp. to obtain pure colonies as described previously by Singh and Barnard [ 1 ]. From the pure colonies, the V. cholerae were then grown in nutrient broth (pH 7) (BioLab) to the late stationary phase to an optical density of ~0.9 OD 600nm .…”
Section: Methodsmentioning
confidence: 99%
“…Intestinal (enteric) pathogenic bacteria, especially Vibrio cholerae ( V. cholerae ), are present in water environments of low temperature and almost neutral pH. In contrast, when these pathogenic bacteria enter a warm-blooded host, through the consumption of contaminated food or water, they will encounter an unfavorable environment such as acidic gastric fluid in the stomach before they reach the intestine to cause an infection [ 1 ]. Once ingested, in order to survive and adapt to the stressful environment, the V. cholerae alters its morphology to accommodate the in vivo cellular requirements [ 2 ].…”
Section: Introductionmentioning
confidence: 99%
See 3 more Smart Citations