Surviving hypoxia without really dying

Boutilier, Robert G.; St-Pierre, Julie

doi:10.1016/s1095-6433(00)00234-8

Cited by 139 publications

(125 citation statements)

References 33 publications

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“…Several subsets of transcripts are characterized by a clear expression decrease, among them the K + and Ca ++ -channels, the Atpase subunits Atp1a3, Atp1b2, the two SERCA transcripts, three glutamate receptors and the glutamate transporter subunit Slc1a3. Certain hypoxia-tolerant lower vertebrate species resort to decreasing ion channels as a mechanism to lower their energetic demands (Boutilier & St-Pierre, 2000). Because macromolecule turnover and ion-motive ATPases are major ATP consumers it comes as no surprise in the present www.ccsenet.org/jmbr Journal of Molecular Biology Research Vol.…”

Section: Down-regulated Transcriptsmentioning

confidence: 99%

“…The energy demand of the inner ear is high and can be compared to that of brain tissue. To meet the energy demand under conditions of inflammation and hypoxia, anaerobic ATP supply is triggered (Pasteur effect; Boutilier & St-Pierre, 2000). ATP production via glycolysis is associated with increased glucose transport and consumption (Frezza et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Gene Expression Patterns in Functionally Different Cochlear Compartments of the Newborn Rat

Gross¹,

Mazurek²

2015

JMBR

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In an experimental model of organotypic cultures of the stria vascularis (SV), the organ of Corti (OC) and the modiolus (MOD), we compared the expression levels and injury/hypoxia induced response of 36 genes associated with the cells´ energy-producing and energy-consuming processes, using the microarray technique. A decrease of expression was observed for most of the voltage-dependent K + -and Ca ++ -channels as an effective mechanism to lower energetic demands. We identified two gene networks of transcripts that are differentially expressed across the three regions. One cluster is associated with the transcription factor hypoxia-inducing factor (Hif-1a) and the second one with the caspase and calpain cell death genes Casp3, Capn1, Capn2 and Capns1. The Hif-1a gene subset consists of genes belonging to the glucose metabolism (glucose transporter Slc2a1, glycolytic enzymes Gapdh, Hk1 and Eno2), the Na + /K + homeostasis (ATPase Atp1a1) and the glutamate pathway (NMDA receptor associated protein 1 Grina, glutamate transporter Slc1a1, Slc1a3). The Slc2a1, Gapdh, Hk1, Slc1a3, Grina and Atp1a1 transcripts are also members of the cell death subset indicating a role they have to play in the differential regional cell death rates. The newly identified genes Grina and calnexin (Canx) may play specific and yet unknown roles in regulating cell death induced by injury and hypoxia in the inner ear. We assume that the differential regional response occurs on the basis of endogenous gene regulatory mechanisms and may be important to maintaining the cochlea's function following damage from trauma and hypoxia.

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Section: Down-regulated Transcriptsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gene Expression Patterns in Functionally Different Cochlear Compartments of the Newborn Rat

Gross¹,

Mazurek²

2015

JMBR

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“…At late stage the symptoms of tissue hypoxia were observed. Suppressed NAD-dependent aerobic pathway of cellular oxidation switches over more rational succinate-oxidase and alternative pentosephosphate way of glucose consumption (Zarubina, 1999;Hochachka et al, 1996;Boutilier et al, 2000). Oxygen lack in blood leads to intensive high energy phosphate utilization.…”

Section: The Treatment Of Hypoxia By Redox Active Agentmentioning

confidence: 99%

The Inflammatory Response of Respiratory System to Metal Nanoparticle Exposure and Its Suppression by Redox Active Agent and Cytokine Therapy

Nikolaev¹,

Mikhalev²,

Marchenko³

et al. 2012

Recent Trend in Electrochemical Science and Technology

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“…If oxygen concentrations are inadequate, cell proliferation ceases and cell viability begins to break down. Indeed, under hypoxic conditions (that is in low oxygen concentration conditions), cells convert glucose to lactic acid (Boutilier and St-Pierre, 2000) and necrosis begins to occur. Tolerance to hypoxic conditions differs widely between cell types.…”

Section: Introductionmentioning

confidence: 99%

“…Tolerance to hypoxic conditions differs widely between cell types. Some cells can survive for several hours under mild hypoxic conditions, but only for several minutes under zero oxygen conditions (Boutilier and St-Pierre, 2000). Success in growing new tissue on scaffolds has been limited to sizes of only a few hundred micrometres, because of the inadequacy of the oxygen supply.…”

Section: Introductionmentioning

confidence: 99%

Cell Proliferation and Oxygen Diffusion in a Vascularising Scaffold

Landman

Cai

2007

Bull. Math. Biol.

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The supply of oxygen to proliferating cells within a scaffold is a key factor for the successful building of new tissue in soft tissue engineering applications. A recent in vivo model, where an arteriovenous loop is placed in a scaffold, allows a vascularising network to form within a scaffold, establishing an oxygen source within, rather than external, to the scaffold. A one-dimensional model of oxygen concentration, cell proliferation and cell migration inside such a vascularising scaffold is developed and investigated. In addition, a vascularisation model is presented, which supports a vascularisation front which moves at a constant speed. The effects of vascular growth, homogenous and heterogenous seeding, diffusion of cells and critical hypoxic oxygen concentration are considered. For homogenous seeding, a relationship between the speed of the vascular front and a parameter defining the rate of oxygen diffusion relative to the rate of oxygen consumption determines whether a hypoxic region exists at some time. In particular, an estimate of the length of time that a fixed point in the scaffold will remain under hypoxic conditions is determined. For heterogenous seeding, a Fisher-like travelling wave of cells is established behind the vascular front. These findings provide a fundamental understanding of the important interplay between the parameters and allows for a theoretical assessment of a seeding strategy in a vascularising scaffold.

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Surviving hypoxia without really dying

Cited by 139 publications

References 33 publications

Gene Expression Patterns in Functionally Different Cochlear Compartments of the Newborn Rat

Gene Expression Patterns in Functionally Different Cochlear Compartments of the Newborn Rat

The Inflammatory Response of Respiratory System to Metal Nanoparticle Exposure and Its Suppression by Redox Active Agent and Cytokine Therapy

Cell Proliferation and Oxygen Diffusion in a Vascularising Scaffold

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