Survivin mRNA in peripheral blood is frequently detected and significantly decreased following resection of gastrointestinal cancers

Abstract

This is the first report showing that direct quantitative real-time RT-PCR analysis of survivin mRNA expression in peripheral blood of patients with gastrointestinal cancers is technically feasible. Survivin mRNA levels fall significantly following complete resection and might become a molecular marker for the completeness of surgical resection.

Dihydropyrimidine dehydrogenase mRNA expression in peripheral blood of rectal cancer patients is significantly associated with residual tumor and distant metastases following resection

“…These findings differ from our results on survivin mRNA detection in peripheral blood [9]. It is however worth mentioning that DPD and TS are excessively more ubiquitously expressed than for example survivin, which seems to be more tumor cell specific.…”

“…PCR conditions were as previously described [9]. The detection of amplified cDNA resulted in a cycle threshold (C t ) value which was inversely proportional to the amount of cDNA.…”

“…It has recently been discussed whether direct quantitative real-time RT-PCR assays (qRT-PCR, TaqMan) can be used to detect circulating tumor mRNA in whole blood samples after tumor cell enrichment prior to RNA extraction [9]. In this pilot study we sought to evaluate whether DPD and TS mRNA expression levels in peripheral blood from patients with rectal cancer can be detected and if they show associations to the primary tumor expansion, regional lymph node metastases, grading, distant metastases, and residual tumor category (R-category).…”

Quantitative analysis of Survivin RNA expression in blood as a non‐invasive predictor of response to neoadjuvant radiochemotherapy in esophageal cancer

“…However, all of these potential molecular response markers are of invasive nature, and therefore impractical for monitoring of therapy success during treatment. Hoffmann et al [20] have recently successfully reported a method for quantification of RNA expression from peripheral blood. Subsequently, it has been shown that the expression level of Excision Repair Cross-Complementing 1 (ERCC1) in peripheral blood is significantly associated with a minor response to therapy in patients with locally advanced cancer of the esophagus, suggesting this approach a powerful new tool for bloodbased response prediction [21].…”

Crosstalk between CTC, Immune System and Hypoxic Tumor Microenvironment

“…CTC are reported to express survivin [49,17] an antiapoptotic protein that confers resistance to cytotoxic therapies and cytotoxic effectors [45]. Survivin expression in CTC may thus promote immune escape.…”