Survivin is a prognostic indicator in glioblastoma and may be a target of microRNA‑218

Abstract

Accumulating evidence has revealed that survivin expression is associated with a malignant phenotype and poor prognosis in glioma. Survivin is also a potential target of microRNA (miRNA/miR)-218. The aim of the present study was to investigate the expression and function of survivin in glioblastoma, and to examine the association between survivin and miR-218. For that purpose, survivin mRNA levels were analyzed in 144 frozen samples of glioblastoma using whole-genome RNA sequencing. In vitro cell proliferation, migration, invasion and apoptosis assays were performed, and survivin expression was detected by western blotting. The results revealed that the mRNA expression levels of survivin were negatively and significantly associated with overall survival in glioblastoma. Further in vitro analyses suggested that miR-218 may inhibit the expression of survivin. Expression of miR-218 in the LN229 cell line was significantly lower than that in the immortalized human gliocyte HEB cell line. miR-218 markedly inhibited tumor cell proliferation, migration and invasion capacities, and decreased apoptosis. miR-218 also inhibited the expression of survivin. These results indicated that survivin may be a target of miR-218 and could serve as a predictive biomarker.

<p>miR-596 suppresses the expression of Survivin and enhances the sensitivity of osteosarcoma cells to the molecular targeting agent anlotinib</p>

“…Besides miR-596, microRNA-330-5p, microRNA-218, miR-542-3p, or miR-494 could also target Survivin and inhibit the proliferation or metastasis in some other cancers, such as gastric cancer. [65][66][67][68] It is valuable to examine the expression of the miRs in clinical tissues. TCGA is a common database; however, there are no data about Survivin-OSA in TCGA.…”

FAM72, Glioblastoma Multiforme (GBM) and Beyond

“…Along with FAM72A, the FOXM1 protein was also found to be similarly co-regulated with BIRC5 [ 108 ]. BIRC5 expression is high in most tumors; however, its usefulness as a prognostic marker is still a controversial issue [ 110 , 111 ].…”

Antitumor Activity of Curcumin in Glioblastoma

“…Survivin, or baculoviral inhibitor of apoptosis (BIRC5), is a protein belonging to the inhibitor of apoptosis (IAP) family of proteins; it is expressed in embryonal tissues, upregulated in human cancer cells and absent in most normal tissue [ 24 ]. Survivin has multiple functions including cell cycle regulation as well as inhibition of apoptosis and has been shown to induce chromosomal instability [ 25 ]. In a recent study, 144 patients with GBM underwent whole-genome sequencing and prognosis of the group with high survivin expression was found to be poor, compared to those with lower expression [ 25 ].…”

“…Survivin has multiple functions including cell cycle regulation as well as inhibition of apoptosis and has been shown to induce chromosomal instability [ 25 ]. In a recent study, 144 patients with GBM underwent whole-genome sequencing and prognosis of the group with high survivin expression was found to be poor, compared to those with lower expression [ 25 ]. Patient-derived GBM cells and established human GBM cell lines (U87, U51, U235) treated with 25 μM curcumin have been shown to have increased expression of phosphorylated ERK, p38 and JNK (via c-Jun) at one and six hours.…”