Survivin Downregulation Is Associated with Vasectomy-Induced Spermatogenic Damage and Apoptosis

Al‐Maghrebi, May; Kehinde, Elijah O.; Anim, Jehoram T.

doi:10.1159/000324551

Cited by 9 publications

(7 citation statements)

References 41 publications

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“…It is also shown that spermatocytes that fail to complete their mitotic division are removed by apoptosis [48]. Previous studies showed induction of apoptosis in male germ cell by testosterone withdrawal [49] and after vasectomy [50]. So, the decreased thickness of germinal epithelium in our study, could be explained by the increased apoptosis due decreased testosterone.…”

Section: Discussionsupporting

confidence: 60%

Light and electron microscopic study on the effect of antischizophrenic drugs on the structure of seminiferous tubules of adult male albino rats

Soliman

Wagih

Attia

et al. 2015

Folia Histochem Cytobiol.

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Introduction. Sexual dysfunction and infertility are symptoms which have been rarely studied in patients treated with antischizophrenic drugs, aripiprazole and olanzapine, for long period. This work aimed to investigate the effects of aripiprazole and olanzapine on the structure of seminiferous tubules of rats at both light microscopic and ultrastructural levels. Material and methods. Sixty adult male rats were divided into 3 groups (n = 20): control group (Group I) and two experimental ones (II and III). Rats in Group II received 2 mg/kg/day aripiprazole while rats in Group III received 0.5 mg/kg/day olanzapine for 14 weeks. Thereafter, testis were removed and processed for both light and electron microscopic study. Qualitative morphological analyses and histomorphometric measurements of seminiferous tubules were performed. Results. Rats in Group II showed reduction of testicular weight, seminiferous tubules' diameter, epithelial height, spermatogenic count, spermatogenic index and spermatogenic score whereas Sertoli cells count was increased. Olanzapine-treated rats also showed epithelial desquamation, separation and apoptotic changes of germ cells. Sertoli cells showed vacuolization, dilatation of smooth endoplasmic reticulum and accumulation of lipid droplets. Abnormality in the shape and structure of late spermatids and presence of giant cells were also demonstrated. Aripiprazole induced less adverse histological changes in rat testis than olanzapine. Conclusions. Olanzapine followed by aripiprazole had adverse histological effects on the structure of the seminiferous tubules, which may affect spermatogenesis.

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Section: Discussionsupporting

confidence: 60%

Light and electron microscopic study on the effect of antischizophrenic drugs on the structure of seminiferous tubules of adult male albino rats

Soliman

Wagih

Attia

et al. 2015

Folia Histochem Cytobiol.

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“…So far, current knowledge indicates that, besides NAIP, survivin is highly expressed in adult human testis, especially in the nuclei of mature spermatocytes, and its downregulation is linked with spermatogenic failure [ 39 – 41 ]. Moreover, recent experimental evidences indicated that survivin could be associated with a direct control of cell survival: it acts as protein inhibitor of apoptosis by inhibiting caspases and antagonizing mitochondria-dependent apoptosis [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Role of Inhibitors of Apoptosis Proteins in Testicular Function and Male Fertility: Effects of Polydeoxyribonucleotide Administration in Experimental Varicocele

Minutoli

Arena

Antonuccio

et al. 2015

BioMed Research International

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Neuronal apoptosis inhibitory protein (NAIP) and survivin might play an important role in testicular function. We investigated the effect of PDRN, an agonist of adenosine A2A receptor, on testicular NAIP and survivin expression in an experimental model of varicocele. After the creation of experimental varicocele (28 days), adolescent male Sprague-Dawley rats were randomized to one of the following treatments lasting 21 days: vehicle, PDRN (8 mg/kg i.p., daily), PDRN + 3,7-dimethyl-propargylxanthine (DMPX, a specific adenosine A2A-receptor antagonist, 0.1 mg/kg i.p., daily), varicocelectomy, and varicocelectomy + PDRN (8 mg/kg i.p., daily). Sham-operated animals were used as controls. Animals were then euthanized and testis expression of NAIP and survivin was evaluated through qRT-PCR, western blot, and immunohistochemical analysis. Spermatogenetic activity was also assessed. NAIP and survivin expressions were significantly reduced following varicocele induction when compared to sham animals whereas PDRN-treated rats showed an increase in NAIP and survivin levels. Immunohistochemistry revealed an enhanced expression of NAIP and survivin with a characteristic pattern of cellular localization following PDRN treatment. Moreover, administration of PDRN significantly restored spermatogenic function in varicocele rats. PDRN may represent a rational therapeutic option for accelerating recovery from depressed testicular function through a strategic modulation of apoptosis in experimental varicocele.

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“…Al-Maghrebi et al . [30] found that the vasectomy led to apoptosis of the germinal cells. Moreover, Kong et al .…”

Section: Discussionmentioning

confidence: 99%

Comparative study between the excision-ligation and autoligation of vas deferens technique for teaser rams preparation

2019

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Aim: The present study was designed to demonstrate the autoligation (AL) of vas deferens and the excision-ligation (EL) technique to generate vasectomized rams to reduce the complications, operative time, and price of the vasectomy techniques. Materials and Methods: A total of 12 healthy and mature Iraqi Awassi rams were used, which divided into two groups, six rams for each one. The former group was performed the EL technique while the latter group, the AL of vas deferens technique was used. Results: The results of the present study found that both techniques were same with the reproductive efficient examinations that mean the two techniques had same ability to close the male genital passage for teaser rams preparation. However, the methods were different with the histopathological changes, operation time, prices, and complications, which were minor in the AL of vas deferens compared with the EL technique. Conclusion: The AL technique of vas deferens to prepare teaser animal is recommended over the EL technique due to different aspects such as cost, fewer complications, and active teaser for a long period are the main aspects of AL technique.

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Survivin Downregulation Is Associated with Vasectomy-Induced Spermatogenic Damage and Apoptosis

Cited by 9 publications

References 41 publications

Light and electron microscopic study on the effect of antischizophrenic drugs on the structure of seminiferous tubules of adult male albino rats

Light and electron microscopic study on the effect of antischizophrenic drugs on the structure of seminiferous tubules of adult male albino rats

Role of Inhibitors of Apoptosis Proteins in Testicular Function and Male Fertility: Effects of Polydeoxyribonucleotide Administration in Experimental Varicocele

Comparative study between the excision-ligation and autoligation of vas deferens technique for teaser rams preparation

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