Survivin: A new target for anti-cancer therapy

Ryan, Bríd M.; O’Donovan, Norma; Duffy, Michael J.

doi:10.1016/j.ctrv.2009.05.003

Cited by 348 publications

(315 citation statements)

References 162 publications

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“…Ternary systems produced through the addition of dioleoyltrimethylammoniumpropane: dioleoylphosphatidylethanolamine (DO-TAP:DOPE) liposomes to the CPP-nucleic acid complexes were also assayed since previous work had revealed that the association of these vesicles with CPP complexes led to the formation of more efficient plasmid delivery systems. 11,12 Our results showed that the histidine tail preserves or improves the S4 13 -PV peptide's ability to efficiently deliver reporter plasmid DNA and siRNAs to different cell lines. In order to test the efficiency of the new CPP-based delivery systems to silence endogenous genes, the survivin gene was selected as a target.…”

Section: ■ Introductionmentioning

confidence: 74%

“…Moreover, survivin renders malignant cells resistant to conventional antitumoral treatments, by counteracting pro-apoptotic stimuli and, therefore, enhancing cancer cell survival. 11,21−25 Altogether, the present findings open a new window to extend the use of CPPs, including the S4 13 -PV peptide and its histidine derivative, as appropriate tools for nucleic acid delivery, with potential for clinical applications in different pathologies, such as cancer.…”

Section: ■ Introductionmentioning

confidence: 83%

“…The transfection efficiency showed the dependence on the number of histidine residues and on their position in the sequence, as well as on the pH at which the peptides change from an in-plane to a transmembrane alignment, the most efficient peptides being those containing 4 to 5 histidines located in the polar face of the α− helix structure. 9 On the basis of all these findings, modifications were performed on the S4 13 -PV peptide, a dermaseptin derived peptide fused to the nuclear localization sequence of the SV40 large T antigen, 10 which has been extensively studied in our laboratory. In this regard, a S4 13 -PV analogue with a fivehistidine tail at the N-terminus was synthesized, originating a new peptide called herein H 5 -S4 13 -PV.…”

Section: ■ Introductionmentioning

confidence: 99%

“…9 On the basis of all these findings, modifications were performed on the S4 13 -PV peptide, a dermaseptin derived peptide fused to the nuclear localization sequence of the SV40 large T antigen, 10 which has been extensively studied in our laboratory. In this regard, a S4 13 -PV analogue with a fivehistidine tail at the N-terminus was synthesized, originating a new peptide called herein H 5 -S4 13 -PV. Following our previous work on the development of S4 13 -PV peptide-based vectors for nucleic acid delivery 9,10 and aiming at identifying the molecular features responsible for efficient nucleic acid delivery, the new peptide was here compared with the wild-type (S4 13 -PV) and its scrambled (S4 13 -PV scr ) and reverse NLS (S4 13 -PV rev ) analogues (Table 1), in terms of its ability to efficiently deliver plasmid DNA and siRNA to different cell lines.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In this regard, a S4 13 -PV analogue with a fivehistidine tail at the N-terminus was synthesized, originating a new peptide called herein H 5 -S4 13 -PV. Following our previous work on the development of S4 13 -PV peptide-based vectors for nucleic acid delivery 9,10 and aiming at identifying the molecular features responsible for efficient nucleic acid delivery, the new peptide was here compared with the wild-type (S4 13 -PV) and its scrambled (S4 13 -PV scr ) and reverse NLS (S4 13 -PV rev ) analogues (Table 1), in terms of its ability to efficiently deliver plasmid DNA and siRNA to different cell lines. Ternary systems produced through the addition of dioleoyltrimethylammoniumpropane: dioleoylphosphatidylethanolamine (DO-TAP:DOPE) liposomes to the CPP-nucleic acid complexes were also assayed since previous work had revealed that the association of these vesicles with CPP complexes led to the formation of more efficient plasmid delivery systems.…”

Section: ■ Introductionmentioning

confidence: 99%

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Comparison of the Efficiency of Complexes Based on S4₁₃-PV Cell-Penetrating Peptides in Plasmid DNA and siRNA Delivery

et al. 2013

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The successful application of gene therapy approaches is highly dependent on the efficient delivery of nucleic acids into target cells. In the present study, new peptide-based nonviral systems were developed to enhance plasmid DNA and siRNA delivery, aiming at generating appropriate gene delivery and gene silencing tools for preclinical and clinical application. For this purpose, a new cell-penetrating peptide derived from the wild-type S4 13 -PV peptide was synthesized through the addition of a five-histidine tail to its N-terminus (H 5 -S4 13 -PV), and its ability to mediate gene expression and gene silencing was evaluated and compared to that of the wild-type peptide. The histidine-enriched peptide, H 5 -S4 13 -PV, proved to be generally more efficient and less toxic than the wild-type peptide in the delivery of plasmid DNA. In addition, complexes of H 5 -S4 13 -PV with siRNAs, but not of S4 13 -PV, were efficiently internalized by cells and presented high knockdown activity (63%). Interestingly, systems containing the S4 13 -PV or the H 5 -S4 13 -PV peptide exhibited superior biological activity when compared to those containing the reverse NLS or scrambled peptides, suggesting that both the cell-penetrating sequence and the NLS of the S4 13 -PV peptide influence the competence of binary and ternary complexes to accomplish nucleic acid delivery. In order to unravel the cancer therapeutic potential of formulations with the histidine-enriched peptide, their efficiency to mediate silencing of the oncogenic protein survivin was evaluated. As opposed to complexes with the wild-type peptide, H 5 -S4 13 -PV complexes showed the ability to promote a high survivin knockdown at the level of both protein (44%) and mRNA (73%), in HT1080 cells.

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Section: ■ Introductionmentioning

confidence: 74%

Section: ■ Introductionmentioning

confidence: 83%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparison of the Efficiency of Complexes Based on S4₁₃-PV Cell-Penetrating Peptides in Plasmid DNA and siRNA Delivery

et al. 2013

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Rational Design of Multifunctional Upconversion Nanocrystals/Polymer Nanocomposites for Cisplatin (IV) Delivery and Biomedical Imaging

Xiao

et al. 2013

Advanced Materials

131

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By combining upconversion nanoparticles with the cisplatin (IV) prodrug we have demonstrated that a stable and multifunctional drug delivery system can be designed that will both reduce the drawbacks of cisplatin and give insight in to its in vitro/in vivo imaging. The up/down-conversion fluorescence are detectable and show obvious co-localization, demonstrating that the nanoparticles are rather stable inside cells and retain the UCNPs and block copolymer.

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Early gene expression changes by Epstein‐Barr virus infection of B‐cells indicate CDKs and survivin as therapeutic targets for post‐transplant lymphoproliferative diseases

Bernasconi

Ueda

Krukowski

et al. 2013

Intl Journal of Cancer

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Lymphoproliferative diseases (LPDs) associated with Epstein-Barr virus (EBV) infection cause significant morbidity and mortality in bone marrow and solid organ transplant recipients. To gain insight into LPD pathogenesis and to identify potential effective therapeutic approaches, we investigated early molecular events leading to B-cell transformation by gene expression profiling of EBV-infected B-cells from tonsils by Affymetrix microarray 72 hr postinfection when the B-cells hyperproliferation phase starts. Cell cycle and apoptosis were the most significantly affected pathways and enriched gene sets. In particular, we found significantly increased expression of cyclin-dependent kinase (CDK)1 and CCNB1 (cyclin B1) and of one of their downstream targets BIRC5 (survivin). Importantly, the strong upregulation of the antiapoptotic protein survivin was confirmed in lymphoblastoid cell lines (LCLs) and 71% of EBV-positive post-transplant EBV-LPD lesions scored positive for survivin. The validity of early transforming events for the identification of therapeutic targets for EBV-LPD was confirmed by the marked antiproliferative effect of the CDK inhibitor flavopiridol on LCLs and by the strong induction of apoptosis by survivin inhibition with YM155 or terameprocol. Our results suggest that targeting of CDKs and/or survivin in post-transplant EBV-LPD by specific inhibitors might be an important approach to control and eliminate EBV-transformed B-cells that should be further considered.Lymphoproliferative disease (LPD) associated with the B-cell tropic Epstein-Barr virus (EBV) causes significant morbidity and mortality in solid organ transplant (SOT) 1 or bone marrow transplant (BMT) 2 recipients. The risk to develop EBV-LPD is greatest in EBV-seronegative transplant recipients, i.e., upon primary EBV infection, and in patients with extensive iatrogenic immunosuppression. 3 EBV-associated B-cell lymphoma in the setting of post-transplant LPD (PTLD) is

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Survivin: A new target for anti-cancer therapy

Cited by 348 publications

References 162 publications

Comparison of the Efficiency of Complexes Based on S4₁₃-PV Cell-Penetrating Peptides in Plasmid DNA and siRNA Delivery

Comparison of the Efficiency of Complexes Based on S4₁₃-PV Cell-Penetrating Peptides in Plasmid DNA and siRNA Delivery

Rational Design of Multifunctional Upconversion Nanocrystals/Polymer Nanocomposites for Cisplatin (IV) Delivery and Biomedical Imaging

Early gene expression changes by Epstein‐Barr virus infection of B‐cells indicate CDKs and survivin as therapeutic targets for post‐transplant lymphoproliferative diseases

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Survivin: A new target for anti-cancer therapy

Cited by 348 publications

References 162 publications

Comparison of the Efficiency of Complexes Based on S413-PV Cell-Penetrating Peptides in Plasmid DNA and siRNA Delivery

Comparison of the Efficiency of Complexes Based on S413-PV Cell-Penetrating Peptides in Plasmid DNA and siRNA Delivery

Rational Design of Multifunctional Upconversion Nanocrystals/Polymer Nanocomposites for Cisplatin (IV) Delivery and Biomedical Imaging

Early gene expression changes by Epstein‐Barr virus infection of B‐cells indicate CDKs and survivin as therapeutic targets for post‐transplant lymphoproliferative diseases

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Product

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Comparison of the Efficiency of Complexes Based on S4₁₃-PV Cell-Penetrating Peptides in Plasmid DNA and siRNA Delivery

Comparison of the Efficiency of Complexes Based on S4₁₃-PV Cell-Penetrating Peptides in Plasmid DNA and siRNA Delivery