Survival Times of Campylobacter coli in Sterilized Buffalo Milk

Abstract

The survival level of five Campylobacter coli strains isolated from bovine faeces was studied in sterilized buffalo milk kept at 4°C under aerobic conditions. All strains died between 21 and 30 h of storage.

Growth capacity of thermotolerant campylobacters in culture media supplemented with pig and cow blood

“…Suspected colonies were identified to species level by their morphological characteristics, susceptibility to cephalothin and nalidixic acid, growth at 26, 37 or 42ºC and their biochemical profiles using the API Campy system (bioMérieux). The growth capacity in different bloods containing media (sheep, pig and cow blood) was assessed by viable counts of each strain using the modified Miles and Misra method (Tresierra-Ayala et al, 1999). In brief, bacterial suspensions of each strain were prepared in distilled water (3 x 10 8 CFU/ml); log 10 serially diluted in 0.1% peptone water and 20µl from each dilution were seeded, in quintuplicate, onto blood agar plates.…”

Evaluación de un medio de cultivo para el aislamiento selectivo de campylobacters termotolerantes

“…Las cepas identificadas como campylobacters termotolerantes, fueron sembradas, por duplicado, en el medio Skirrow modificado y medio de cultivo evaluado, mediante la técnica de microrrecuento de Miles y Misra modificada [1], para determinar la capacidad de crecimiento de las cepas de Campylobacter termotolerantes…”

“…Se logró aislar e identificar 20 cepas (13 de Campylobacter jejuni y 7 de Campylobacter coli) de un total de 70 muestras, determinándose que no había diferencia estadísticamente significativa respecto a la capacidad de aislamiento entre el medio de cultivo evaluado y el medio Skirrow modificado. Para el análisis comparativo de la cuantificación del crecimiento de las cepas en ambos medios, se empleó la técnica de Miles y Misra modificada [1] y se observó que las cepas no mostraban diferencia estadísticamente significativa en cuanto a su crecimiento en los medios ensayados, obteniéndose recuentos promedios de 23,4 x10 6 y 21,7x10 6 UFC/ml, para la especie de C. coli en el medio de cultivo evaluado y el medio Skirrow modificado y de 44,6 x10 6 y 40,1 x10 6 UFC/ml para la especie C. jejuni en ambos medios de cultivo, respectivamente. El medio de cultivo evaluado permite la misma capacidad de aislamiento selectivo para campylobacters termotolerantes que el medio Skirrow modificado; además, estos agentes microbianos crecen cuantitativamente de modo similar en ambos medios de cultivo.…”

“…Twenty strains (13 C. coli y 7 C. jejuni) were isolated and identified from a total of 70 samples There was not significant difference with respect to the isolation ability between the culture medium evaluated and the modified Skirrow medium. For the comparative analysis about quantification of the growth of strains in both media, Miles and Misra technique, modified [1], was employed. So, it was determined that the strains showed no significant difference with respect to their growth on both media studied.…”

Survival rates of thermotolerant Campylobacter species in a transport and enrichment medium under different environmental conditions

“…Viable counts, carried out using a modified Miles and Misra method (Tresierra-Ayala et al, 1999) were done on 0, 1, 3, 5, 7 and 9 days after inoculation or until no bacterial survival was detected. At selected days, a 0.1ml sample of TEC medium from each atmospheric conditions was obtained, log 10 serially diluted in 0.1% peptone water and 20µl from each dilution were seeded in quintuplicate on blood agar plates.…”