Survival, re-expansion, and pregnancy outcome following vitrification of dromedary camel cloned blastocysts: A possible role of vitrification in improving clone pregnancy rate by weeding out poor competent embryos

Moulavi, F.; Soto-Rodriguez, Sofia; Kuwayama, Masashige; Asadi-Moghaddam, B.; Hosseini, Sayyed Morteza

doi:10.1016/j.cryobiol.2019.08.002

Cited by 7 publications

(3 citation statements)

References 39 publications

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“…There is renewed interest in camelid cloning, with various techniques being attempted [5][6][7] . Eleven healthy mature individuals were selected from the three interest groups (breeds), and tissue samples were collected for culture.…”

mentioning

confidence: 99%

Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

Olsson¹,

Tinson²,

Shamsi³

et al. 2021

Sci Rep

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Cloning, through somatic cell nuclear transfer (SCNT), has the potential for a large expansion of genetically favorable traits in a population in a relatively short term. In the present study we aimed to produce multiple cloned camels from racing, show and dairy exemplars. We compared several parameters including oocyte source, donor cell and breed differences, transfer methods, embryo formation and pregnancy rates and maintenance following SCNT. We successfully achieved 47 pregnancies, 28 births and 19 cloned offspring who are at present healthy and have developed normally. Here we report cloned camels from surgical embryo transfer and correlate blastocyst formation rates with the ability to achieve pregnancies. We found no difference in the parameters affecting production of clones by camel breed, and show clear differences on oocyte source in cloning outcomes. Taken together we demonstrate that large scale cloning of camels is possible and that further improvements can be achieved.

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confidence: 99%

Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

Olsson¹,

Tinson²,

Shamsi³

et al. 2021

Sci Rep

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“…In addition, increased ROS during embryo development has been associated with decreased blastocyst formation (Yang et al, 2018). In other words, regulating ROS production is important to improve blastocyst competence (Moulavi et al, 2019) and survival of vitrified blastocysts (Pereira et al, 2019). During cryopreservation, blastocysts can be damaged by various factors such as membrane, DNA, and/or thermal damage (Inaba et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…As one experimental method, cryopreservation was carried out by vitrification with a Cryotop (Kitazato Supply Co, Fujinomiya, Japan, Fig. 1A) using a slightly modified version of the procedure described by Moulavi (2019). 20% FBS) at room temperature.…”

Section: Vitrification and Warming Proceduresmentioning

confidence: 99%

Comparison of Cryotop and ReproCarreir products for cryopreservation of bovine blastocysts through survival rate and blastocysts quality

Kim

Yang

Park

et al. 2020

J Anim Reprod Biotechnol

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Cryopreservation of oocytes and embryos in assisted reproductive technology for application in domestic animals and humans is very important to preserve the maternal and paternal genetic complements. Cryopreservation allows widespread use of valuable animal embryos to improve the chances of pregnancy. However, intracellular ice formation in cryopreservation methods for oocytes and embryos can lead to fatal damage. To overcome this issue of intracellular ice formation, slow freezing and vitrification methods are most widely used (Mucci et al., 2006).The balance of redox reactions in cells is very crucial for maintaining the metabolic environment and gene expression during embryonic development. However, high levels of reactive oxygen species (ROS) were seen in preimplantation embryos of cattle (Min et al., 2014). ROS, such as hydrogen peroxide, cause oxidative stress, which is known to cause DNA damage and apoptosis (Takahashi, 2012). Therefore, regulating oxidative stress and ROS production during the vitrification-warming process is criti-

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A Modified Handmade Cloning Method for Dromedary Camels

Moulavi

Hosseini

2023

Methods in Molecular Biology

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Survival, re-expansion, and pregnancy outcome following vitrification of dromedary camel cloned blastocysts: A possible role of vitrification in improving clone pregnancy rate by weeding out poor competent embryos

Cited by 7 publications

References 39 publications

Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

Comparison of Cryotop and ReproCarreir products for cryopreservation of bovine blastocysts through survival rate and blastocysts quality

A Modified Handmade Cloning Method for Dromedary Camels

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