Survival of pathogenic bacteria during mesophilic anaerobic digestion of animal waste

Kearney, Theresa E.; Larkin, Michael J.; Frost, J. P.; Levett, Paul N.

doi:10.1111/j.1365-2672.1993.tb02768.x

Cited by 84 publications

(51 citation statements)

References 8 publications

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“…Nevertheless, we did isolate 95 Campylobacter isolates from compost, including at later stages of the composting process (i.e., days 15 to 126). Using culture-based enumeration methods, previous studies concluded that campylobacters (primarily C. jejuni bacteria) do not persist well in solid manure (7,11,12,26,32,39) or in thermophilic anaerobic manure digesters (35,36) but can survive for prolonged periods in mesophilic anaerobic digesters (18,19).…”

Section: Discussionmentioning

confidence: 99%

Prolonged Survival ofCampylobacterSpecies in Bovine Manure Compost

Inglis

McAllister

Larney

et al. 2010

Appl Environ Microbiol

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The persistence of naturally occurring campylobacteria in aerobic compost constructed of manure from beef cattle that were administered chlortetracycline and sulfamethazine (AS700) or from cattle not administered antibiotics (control) was examined. Although there were no differences in population sizes of heterotrophic bacteria, the temperature of AS700 compost was more variable and did not become as high as that of control compost. There were significant differences in water content, total carbon (C), total nitrogen (N), and electrical conductivity but not in the C/N ratio or pH between the two compost treatments. Campylobacteria were readily isolated from pen manure, for up to day 15 from control compost, and throughout the active phase of AS700 compost. Campylobacter DNA (including Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis, and Campylobacter jejuni) was detected over the ca. 10-month composting period, and no reductions in quantities of C. jejuni DNA were observed over the duration of the active phase. The utilization of centrifugation in combination with ethidium monoazide (EMA) significantly reduced (>90%) the amplification of C. jejuni DNA that did not originate from cells with intact cell membranes. No differences were observed in the frequency of Campylobacter DNA detection between EMA-and non-EMA-treated samples, suggesting that Campylobacter DNA amplified from compost was extracted from cells with intact cell membranes (i.e., from viable cells). The findings of this study indicate that campylobacteria excreted in cattle feces persist for long periods in compost and call into question the common belief that these bacteria do not persist in manure.

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Section: Discussionmentioning

confidence: 99%

Prolonged Survival ofCampylobacterSpecies in Bovine Manure Compost

Inglis

McAllister

Larney

et al. 2010

Appl Environ Microbiol

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“…Used litter from broiler houses presumably contains large numbers of campylobacteria when removed from the house. Experiments have shown that C. jejuni has a good ability to survive during anaerobic digestion of animal waste (10,11). An earlier study showed that houseflies can function as a vector for C. jejuni when a reservoir is located near the broiler house (20).…”

Section: Discussionmentioning

confidence: 99%

Evidence that Certain Clones of Campylobacter jejuni Persist during Successive Broiler Flock Rotations

Petersen

Wedderkopp

2001

Appl Environ Microbiol

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Through the national surveillance program for Campylobacter spp., nine broiler chicken farms that were infected with Campylobacter jejuni in at least five rotations in 1998 were identified. One additional farm, located at the island of Bornholm where divided slaughter is used extensively, was also selected. Twelve broiler houses located on 10 farms were included in the study. The C. jejuni isolates collected from the selected houses during the surveillance were typed using fla typing and macrorestriction profiling (MRP), and a subset of the isolates, representing each of the identified clones, was serotyped according to the Penner scheme. Pulsed-field gel electrophoresis typing using SmaI and KpnI revealed that the majority of houses (11 of

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“…Kearney et al (50) utilized mesophilic anaerobic digestion to analyze the survival of pathogenic bacteria in animal waste. The group found that viable numbers of E. coli, S. enterica serovar Typhimurium, Yersinia enterocolitica, Listeria monocytogenes, and Campylobacter jejuni were reduced during processing and that indigenous bacterial strains survived better than laboratory strains, consistent with previous results (65,69).…”

Section: Anaerobic and Aerobic Digestionmentioning

confidence: 99%