Survival of Mammals Breathing Organic Liquids Equilibrated with Oxygen at Atmospheric Pressure

Clark, Leland C.; Gollan, Frank

doi:10.1126/science.152.3730.1755

Cited by 735 publications

(232 citation statements)

References 4 publications

Supporting

Mentioning

220

Contrasting

Unclassified

Order By: Relevance

“…1 Further experiments in the 1960s showed the successful use of fluorocarbons at atmospheric pressure for liquid ventilation in cats and mice. 2 Initial experiments with perfluorocarbons (PFC) were performed with spontaneously breathing animals immersed in PFC or with gravity assisted tidal bulk flow of PFC. These techniques were unsuccessful at removing enough CO2.…”

Section: Introductionmentioning

confidence: 99%

Liquid ventilation

Davies¹

1999

J Paediatrics Child Health

View full text Add to dashboard Cite

: Research on using liquid ventilation to provide artificial respiration in mammals has been ongoing since the 1960s. The development of inert perfluorocarbon (PFC) liquids with high oxygen and carbon dioxide solubility has made gas exchange with liquid ventilation possible. In 1991 the technique of partial liquid ventilation was introduced where PFC are instilled into the lungs whilst continuing with conventional mechanical ventilation. Partial liquid ventilation has been shown to improve gas exchange and lung function with decreased secondary lung injury, in animal models of acute lung injury and surfactant deficiency. It has been used in uncontrolled trials in preterm neonates, and preliminary results are available from a randomized controlled trial of partial liquid ventilation in paediatric acute respiratory distress syndrome. Perfluorocarbons can also be used to deliver drugs to the lungs, to lavage inflammatory exudate and debris from the lungs, and as an intrapulmonary X‐ray contrast medium. Many questions about partial liquid ventilation remain unanswered particularly with regard to the dose of PFC required, its ideal method of administration and the long‐term effects. Partial liquid ventilation promises to be an exciting new therapy for infants and children with a variety of respiratory problems. The technique requires ongoing research and experimentation.

show abstract

Section: Introductionmentioning

confidence: 99%

Liquid ventilation

Davies¹

1999

J Paediatrics Child Health

View full text Add to dashboard Cite

show abstract

“…In 1966 Clark and Gollan demonstrated the ability of small mammals to remain alive while breathing oxygenated perfluorocarbons (PFCs) [1]. Since then PFCs have been investigated extensively as an alternative means of respiratory support, and PFCs oxygenated outside the body have been used in the treatment of acute respiratory failure [2,3].…”

Section: Introductionmentioning

confidence: 99%

Hemodynamic effects of partial liquid ventilation with perfluorocarbon in acute lung injury

Houmes¹,

Verbrugge²,

Hendrik³

et al. 1995

Intensive Care Med

View full text Add to dashboard Cite

Objective: To assess the effect of partial liquid ventilation with perfluorocarbons on hemodynamics and gas exchange in large pigs with induced acute lung injury (ALI). Design: Randomized, prospective, double-control, experimental study. Setting: Experimental intensive care unit of a university. Materials: Eighteen large pigs (50 _+ 5 kg body weight) with an average anterior posterior thoracic diameter of 24 cm and induced acute lung injury. Interventions: All animals were surfactant depleted by lung lavage to a PaO2 below 100 mmHg and randomized to receive either perflubron (n = 6) or saline (n = 6) in five intratracheal doses of 5 ml/kg at 20-rain intervals, or no instillation (n = 6). Measurements and results:In all animals heart rate, arterial pressures, pulmonary pressures, cardiac output and blood gases were recorded at 20-rain intervals. There was no deleterious effect on any hemodynamic parameter in the perflubron group, whereas systolic and mean pulmonary arterial pressure values showed a persistent decrease after the first 5 ml/kg of perflubron, from 48.7 _+ 14.1 to 40.8 _+ 11.7 mmHg and from 39.7 _+ 13.2 to 35.2 _+ 12.0 mmHg, respectively. Perflubron resulted in a significant (ANOVA P < 0.01), dose-dependent increase in PaO2 values from 86.3 _+ 22.4 to a maximum of 342.4 _+ 59.4 mmHg at a dose of 25 ml/kg; the other groups showed no significant increase in P.O2. Conclusions: Tracheal instillation of perflubron in induced ALI results in a dose-dependent increase in PaO2 and has no deleterious effect on hemodynamic parameters.

show abstract

“…Perfluorocarbons (PFCs) are fluorinated organic compounds with a great capacity for dissolving high concentration of oxygen and carbon dioxide, making this liquid of interest to perform liquid ventilation (Clark and Gollan 1966). Liquid ventilation has been proposed for a wide range of applications such as bronchial lavage after meconium aspiration (Avoine et al 2011), induction of ultrafast hypothermia after cardiac arrest for brain tissue protection (Darbera et al 2013) and improvement of gas exchanges and lung compliance in adults and infants with acute respiratory distress (Hirschl et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Perfluorocarbon induces alveolar epithelial cell response through structural and mechanical remodeling

Dias¹,

Planus²,

Angely³

et al. 2018

Biomech Model Mechanobiol

View full text Add to dashboard Cite

During total liquid ventilation, lung cells are exposed to perfluorocarbon (PFC) whose chemophysical properties highly differ from standard aqueous cell feeding medium (DMEM). We herein perform a systematic study of structural and mechanical properties of A549 alveolar epithelial cells in order to characterize their response to PFC exposure, using DMEM as control condition. Changes in F-actin structure, focal adhesion density and glycocalyx distribution are evaluated by confocal fluorescent microscopy. Changes in cell mechanics and adhesion are measured by multiscale magnetic twisting cytometry (MTC). Two different microrheological models (single Voigt and power law) are used to analyze the cell mechanics characterized by cytoskeleton (CSK) stiffness and characteristic relaxation times. Cell-matrix adhesion is analyzed using a stochastic multibond deadhesion model taking into account the non-reversible character of the cell response, allowing us to quantify the adhesion weakness and the number of associated bonds. The roles of F-actin structure and glycocalyx layer are evaluated by depolymerizing F-actin and degrading glycocalyx, respectively. Results show that PFC exposure consistently induces F-actin remodeling, CSK softening and adhesion weakening. These results demonstrate that PFC triggers an alveolar epithelial cell response herein evidenced by a decay in intracellular CSK tension, an adhesion weakening and a glycocalyx layer redistribution. These PFC-induced cell adjustments are consistent with the hypothesis that cells respond to a decrease in adhesion energy at cell surface. This adhesion energy can be even further reduced in the presence of surfactant adsorbed at the cell surface.

show abstract

Survival of Mammals Breathing Organic Liquids Equilibrated with Oxygen at Atmospheric Pressure

Cited by 735 publications

References 4 publications

Liquid ventilation

Liquid ventilation

Hemodynamic effects of partial liquid ventilation with perfluorocarbon in acute lung injury

Perfluorocarbon induces alveolar epithelial cell response through structural and mechanical remodeling

Contact Info

Product

Resources

About