Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR

Abstract

ABSTRAKTujuan dari penelitian ini adalah untuk menganalisa daya tahan L. plantarumU40 menggunakan real-time PCR dalam fermentasi rumen in vitro. Desain penelitian yang digunakan adalah Rancangan Acak Kelompok dengan 3 perlakuan dan 4 ulangan. Perlakuan yang diberikan adalah kontrol, inokulasi dengan L. plantarumU40 danL. plantarumU40 + glukosa. Perlakuan dengan inokulasi menghasilkan populasi L. plantarumU40 yang lebih tinggi. Setelah 8 jam inkubasi, pemberian glukosa cenderung menurunkan populasi L. plantarum U40. Perlakuan kontrol menghasilkan populasi L. plantarum U40 yang paling rendah selama fermentasi in vitro.Inokulasi L. plantarum U40meningkatkan populasi Bakteri Asam Laktat secara signifikan (P<0.05) sampai dengan 12 jam inkubasi dibandingkan dengan kontrol. Perlakuan kontrol mempunyai pH yang paling tinggi selama masa inkubasi. Penambahan glukosa secara signifikan (P<0.05) menurunkan pH rumen pada akhir inkubasi (24 jam) (6.30), dibandinkgkan dengan kontrol (6.85).Inokulasi dengan L. plantarumU40 + glukosa secara signifikan (P<0.05) meningkatkan asam propionat, menurunkan asam asetat serta nisbah A/P dibanding perlakuan lainnya.Lactobacillus plantarum U40 tanpa penambahan glukosa tidak memberikan efek terhadap produksi propionat secara signifikan. Dapat disimpulkan bahwa Lactobacillus plantarumU40 dapat bertahan hidup di dalam rumen serta mengubah fermentasi rumen ketika glukosa ditambahkan sebagai sumber karbon. Kata kunci: Lactobacillus plantarumU40, daya tahan, glukosa,fermentasi rumen, quantitative real-time PCR ABSTRACTThe objective of this study was to evaluate the survival of L. plantarumU40 quantified with realtime PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block design with 3 treatments and 4 replications. Treatments were control, rumen fermentation inoculated with L. plantarumU40and L. plantarumU40 + glucose solution. Population of L. plantarum U40 was higher at inoculation treatment. After 8 hours incubation, glucose addition tended to decrease L. plantarum U40 population. Control treatment showed lowest population of L. plantarum U40 along in 184 J.Indonesian Trop.Anim.Agric. 43(2):184-192, June 2018 vitro fermentation compared with other treatment. Inoculation of L. plantarumU40 significantly (p<0.05) increased population of LAB until 12 hours incubation compared with control. Control treatment had highest pH at all incubation time. Glucose addition significantly (P<0.05) decreased final rumen pH (24 hours) (6.30), compared with control treatment (6.85). Inoculation of L. plantarum U40 with glucose addition significantly (P<0.05)increased propionic acid, decreased acetic acid and A/P ratio compared with other treatments. Lactobacillus plantarum U40 without glucose addition did not affect propionic acid production significantly. As conclusion, Lactobacillus plantarum U40 can survive in rumen fluid and changes rumen fermentation when glucose is added as carbon source.

Optimum Level of Lactobacillus plantarum Supplementation as Probiotic on In Vitro Digestibility and Rumen Fermentation Products in Thai Native Cattle

“…This bacterium is native to rumen ecology and binds to the rumen epithelium as well (Stewart, 1992). The research conducted by Astuti et al (2018b) using in vitro gas production technique and real-time PCR concluded that L. plantarum can survive in the rumen. Using 1.5×10 8 CFU/mL commercial strain of L. plantarum in the rumen for in vitro fermentation enhances OMD and total VFA (Direkvandi et al, 2021).…”