Survival of Inoculants in Polluted Sediments: Effect of Strain Origin and Carbon Source Competition

Blumenroth, P.; Wagner‐Döbler, Irene

doi:10.1007/s002489900083

Cited by 17 publications

(6 citation statements)

References 38 publications

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“…Although McClure et al (27) could establish a stable population of the introduced strain in the sludge environment, no enhanced degradation of chlorobenzoate was observed. Different authors proposed that the inability of the inoculated strains to degrade the xenobiotics may have been due to the availability of alternative substrates (5,15,27,28,36,44). However, C. testosteroni I2gfp received daily only 40 mg of 3-CA/liter together with 1 g of COD per liter (diluted milk powder) and performed its specific activity within 2 weeks.…”

Section: Discussionmentioning

confidence: 99%

“…However, under natural conditions, these laboratory strains have to compete with the established microbial community, resulting in a decrease of the amount of inoculated cells (15). This competition can be controlled by adding a carbon source that the inoculant can degrade (5) or by changing operation parameters (14). Thus far in water treatment, only a few successful cases of small-scale bioaugmentation in activated sludge by using natural or genetically modified microorganisms have been described.…”

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confidence: 99%

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Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2 gfp

Boon

Goris

Vos

et al. 2000

Appl Environ Microbiol

331

251

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A strain identified as Comamonas testosteroni I2 was isolated from activated sludge and found to be able to mineralize 3-chloroaniline (3-CA). During the mineralization, a yellow intermediate accumulated temporarily, due to the distal meta-cleavage of chlorocatechol. This strain was tested for its ability to clean wastewater containing 3-CA upon inoculation into activated sludge. To monitor its survival, the strain was chromosomally marked with the gfp gene and designated I2gfp. After inoculation into a lab-scale semicontinuous activatedsludge (SCAS) system, the inoculated strain maintained itself in the sludge for at least 45 days and was present in the sludge flocs. After an initial adaptation period of 6 days, complete degradation of 3-CA was obtained during 2 weeks, while no degradation at all occurred in the noninoculated control reactor. Upon further operation of the SCAS system, only 50% 3-CA removal was observed. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes revealed a dynamic change in the microbial community structure of the activated sludge. The DGGE patterns of the noninoculated and the inoculated reactors evolved after 7 days to different clusters, which suggests an effect of strain inoculation on the microbial community structure. The results indicate that bioaugmentation, even with a strain originating from that ecosystem and able to effectively grow on a selective substrate, is not permanent and will probably require regular resupplementation.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2 gfp

Boon

Goris

Vos

et al. 2000

Appl Environ Microbiol

331

251

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show abstract

“…The research on aquifers contaminated with chlorinated ethenes illustrates that traditional techniques such as cell bioaugmentation can be effectively used to remediate a site if the inoculant can fill a niche in the environmental ecosystem 21 -in this case, the use of the chlorinated ethenes as a terminal electron acceptor. However, the vast majority of the field-scale bioaugmentation experiments have only investigated the cell bioaugmentation approach.…”

Section: B Aquifer Bioaugmentationmentioning

confidence: 99%

New Approaches for Bioaugmentation as a Remediation Technology

Gentry

Rensing

Pepper

2004

Critical Reviews in Environmental Science and Technology

285

124

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“…One solution to this problem, in principle supported by Barac et al [13] and Blumenroth and Wagner-Dobler [14], is that the scientific community coordinates its research efforts on Heirloom microorganisms. The pool of Heirloom species would be chosen based on their amenability to manipulation, exceptional catabolic ability, current knowledge base, tolerance to environmental stresses, technical ease of use and lack of public health threat (e.g.…”

Section: Heirloom Strainsmentioning

confidence: 99%

Perspectives and vision for strain selection in bioaugmentation

Singer

Gast

Thompson

2005

Trends in Biotechnology

128

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Notwithstanding the phenomenally large and ever-increasing resource of pollutant-degrading microbial isolates in laboratories around the globe, inoculum survival remains the 'Achilles' heel' for bioaugmentation of contaminated land. Considerable effort has been invested into inoculum strain selection to facilitate pollutant biodegradation, ranging from the isolation of 'superbugs,' which are microorganisms highly resilient to environmental stresses, harboring catabolically superior pollutant-degrading enzymes, to the other extreme in 'priming', where pollutant degradation is carried out through the addition of soil enriched with an undefined consortium of pollutant-degrading microorganisms.

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Survival of Inoculants in Polluted Sediments: Effect of Strain Origin and Carbon Source Competition

Cited by 17 publications

References 38 publications

Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2 gfp

Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2 gfp

New Approaches for Bioaugmentation as a Remediation Technology

Perspectives and vision for strain selection in bioaugmentation

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