Survival of Salmonella enterica Serovar Newport in Manure and Manure-Amended Soils

Abstract

Salmonella enterica serovar Newport has undergone a rapid epidemic spread in dairy cattle. This provides an efficient mechanism for pathogen amplification and dissemination into the environment through manure spreading on agricultural land. The objective of this study was to determine the survival characteristics of Salmonella serovar Newport in manure and manure-amended soils where the pathogen may be amplified. A multidrug-resistant (MDR) Salmonella serovar Newport strain and a drug-susceptible (DS) strain, both bovine isolates, were inoculated into dairy manure that was incubated under constant temperature and moisture conditions alone or after being mixed with sterilized or nonsterilized soil. Salmonella serovar Newport concentrations increased by up to 400% in the first 1 to 3 days following inoculation, and a trend of steady decline followed. With manure treatment, a sharp decline in cell concentration occurred after day 35, possibly due to microbial antagonism. For all treatments, decreases in Salmonella serovar Newport concentrations over time fit a first-order kinetic model. Log reduction time was 14 to 32 days for 1 log 10 , 28 to 64 days for 2 log 10 , and 42 to 96 days for 3 log 10 declines in the organisms' populations from initially inoculated concentrations. Most-probable-number monitoring data indicated that the organisms persisted for 184, 332, and 405 days in manure, manure-amended nonsterilized soil, and manure-amended sterilized soil, respectively. The MDR strain and the DS strain had similar survival patterns.

Assessment of the content of heavy metals and potential pathogenic microorganisms in soil under illegal dumping sites

“…According to You et al (2006), the environment of the soil does not create optimum conditions for existence of the fecal bacteria. The research performed by Yamahara et al (2012) shows that some of the fecal bacteria multiply inside the soil and adsorb on the particle of the soil or move deeper into the soil.…”

Investigation of Possible Situation of Internalization of Salmonella Enteritidis in Tomato Fruits and Bacterial Survival during Tomato Plant Cultivation

“…Salmonella spp. have been reported to survive from a few days up to 332 days in manure-amended soils (Holley et al, 2006;Islam et al, 2004;You et al, 2006) and S. enterica is known to survive for five weeks in fallow soil (Nicholson et al, 2005;Jensen et al, 2006). In addition, bacteria, which are often found in vegetables and are associated with foodborne outt.…”

Assessing the inactivation of Salmonella in dairy wastewater at varying thermal conditions

“…You et al (2006) studied the inactivation of a multidrug resistant Salmonella serovar Newport strain and a drug susceptible strain in dairy manure and found that the Salmonella count increased during the first 3 d incubation. Subsequently, the Salmonella levels decreased until 35 d incubation.…”

“…The recycling of dairy wastewater (which is likely to have elevated bacterial loads and is stored in ponds) for cleaning the barns is a normal practice which also poses the risk of recirculation of pathogens. Previous research reported that pathogens can survive in dairy wastewater for extended periods of time (Kearney et al 1993;Wang et al 1996;Heinonen-Tanski et al 1998;You et al 2006). …”