Survival of <i>Escherichia coli</i> and <i>Campylobacter jejuni</i> in untreated and filtered lake water

Korhonen, Lauri; Martikainen, Pertti J.

doi:10.1111/j.1365-2672.1991.tb03804.x

Cited by 81 publications

(46 citation statements)

References 21 publications

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“…Therefore, there is considerable interest in understanding its survival and competitiveness in the natural environment. In temperate climates and unpolluted sites, E. coli cells shed into the environment are assumed to be unable to survive and to die quickly (Korhonen and Martikainen, 1991;Bogosian et al, 1996). However, it has been reported that under mesotrophic to eutrophic conditions and (sub)tropical temperatures between 15 and 45 1C this enterobacterium can not only survive but also grow (Hazen and Toranzos, 1990;Tassoula, 1997;Solo-Gabriele et al, 1999).…”

Section: Competitionmentioning

confidence: 99%

In glucose-limited continuous culture the minimum substrate concentration for growth, smin, is crucial in the competition between the enterobacterium Escherichia coli and Chelatobacter heintzii, an environmentally abundant bacterium

2011

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The competition for glucose between Escherichia coli ML30, a typical copiotrophic enterobacterium and Chelatobacter heintzii ATCC29600, an environmentally successful strain, was studied in a carbon-limited culture at low dilution rates. First, as a base for modelling, the kinetic parameters μmax and Ks were determined for growth with glucose. For both strains, μmax was determined in batch culture after different precultivation conditions. In the case of C. heintzii, μmax was virtually independent of precultivation conditions. When inoculated into a glucose-excess batch culture medium from a glucose-limited chemostat run at a dilution rate of 0.075 h−1C. heintzii grew immediately with a μmax of 0.17±0.03 h−1. After five transfers in batch culture, μmax had increased only slightly to 0.18±0.03 h−1. A different pattern was observed in the case of E. coli. Inoculated from a glucose-limited chemostat at D=0.075 h−1 into glucose-excess batch medium E. coli grew only after an acceleration phase of ∼3.5 h with a μmax of 0.52 h−1. After 120 generations and several transfers into fresh medium, μmax had increased to 0.80±0.03 h−1. For long-term adapted chemostat-cultivated cells, a Ks for glucose of 15 μg l−1 for C. heintzii, and of 35 μg l−1 for E. coli, respectively, was determined in 14C-labelled glucose uptake experiments. In competition experiments, the population dynamics of the mixed culture was determined using specific surface antibodies against C. heintzii and a specific 16S rRNA probe for E. coli. C. heintzii outcompeted E. coli in glucose-limited continuous culture at the low dilution rates of 0.05 and 0.075 h−1. Using the determined pure culture parameter values for Ks and μmax, it was only possible to simulate the population dynamics during competition with an extended form of the Monod model, which includes a finite substrate concentration at zero growth rate (smin). The values estimated for smin were dependent on growth rate; at D=0.05 h−1, it was 12.6 and 0 μg l−1 for E. coli and C. heintzii, respectively. To fit the data at D=0.075 h−1, smin for E. coli had to be raised to 34.9 μg l−1 whereas smin for C. heintzii remained zero. The results of the mathematical simulation suggest that it is not so much the higher Ks value, which is responsible for the unsuccessful competition of E. coli at low residual glucose concentration, but rather the existence of a significant smin.

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Section: Competitionmentioning

confidence: 99%

In glucose-limited continuous culture the minimum substrate concentration for growth, smin, is crucial in the competition between the enterobacterium Escherichia coli and Chelatobacter heintzii, an environmentally abundant bacterium

2011

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“…, NO 2 -, and NO 3 -were also found (Table 2). Nutrients have been shown to play an important role in fecal indicator survival in natural systems (Korhonen and Martikainen 1991), probably because high nutrient concentrations prolong the persistence of fecal indicator bacteria or instigate their growth (Findlay et al 2002). Based on the previous reports that measurement of nutrients, particularly nitrate, and fecal indicator bacteria can aid in distinguishing human versus non-human sources of surface water contamination (Peeler et al 2006), observed increases of both fecal ) of dissolved trace elements (Al, As, Ba, Cd, Co, Cr, Cs, Cu, Fe, Li, Mn, Mo, Ni, Pb, Rb, Sb, Sn, Sr, Ti, Tl, U, V) in the Sutla River water at eight sampling sites.…”

Section: Point Source Water Contaminationmentioning

confidence: 99%

Water Quality of Medium Size Watercourse Under Baseflow Conditions: The Case Study of River Sutla in Croatia

Dragun

Kapetanović

Raspor

et al. 2011

AMBIO

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The study on medium size river Sutla in Croatia indicated considerable water contamination at specific sites during the baseflow period, probably associated to low flowrate (0.73-68.8 m 3 s -1 ), and consequently low dilution capacity of this river. Various aspects of contamination were observed: increased conductivity to 1,000 lS cm -1 , decreased dissolved oxygen level to 50%, 4-5°C increased water temperature, increased concentrations of several dissolved trace elements (e.g., maximal values of Li: 45.4 lg l -1 ; Rb: 10.4 lg l -1 ; Mo: 20

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“…From 1998 to 2000, four out of a total of 18 reported waterborne outbreaks in Finland were caused by Campylobacter jejuni contamination (Miettinen et al, 2000). The significance of Campylobacters as a waterborne pathogen is increasing because it can survive for several months in natural waters at low temperatures (Korhonen and Martikainen, 1991). The infectious dose of Campylobacter is rather low (about 500 bacteria cells) (Robinson, 1981;Black et al, 1988), enhancing the likelihood of infection.…”

Section: Outbreaks Related To the Use Of Riverbank-filtrated Drinkingmentioning

confidence: 99%

Removal of Pathogens, Surrogates, Indicators, and Toxins Using Riverbank Filtration

Schijven¹,

Berger²,

Miettinen

Riverbank Filtration

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Survival of Escherichia coli and Campylobacter jejuni in untreated and filtered lake water

Cited by 81 publications

References 21 publications

In glucose-limited continuous culture the minimum substrate concentration for growth, smin, is crucial in the competition between the enterobacterium Escherichia coli and Chelatobacter heintzii, an environmentally abundant bacterium

In glucose-limited continuous culture the minimum substrate concentration for growth, smin, is crucial in the competition between the enterobacterium Escherichia coli and Chelatobacter heintzii, an environmentally abundant bacterium

Water Quality of Medium Size Watercourse Under Baseflow Conditions: The Case Study of River Sutla in Croatia

Removal of Pathogens, Surrogates, Indicators, and Toxins Using Riverbank Filtration

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