Survival of genetically engineered Pseudomonas fluorescens in soil in competition with the parent strain

Elsas, Jan Dirk van; Overbeek, L.S. van; Feldmann, Angelika; Dullemans, A.M.; Leeuw, Olav de

doi:10.1111/j.1574-6941.1991.tb01708.x

Cited by 32 publications

(35 citation statements)

References 20 publications

(35 reference statements)

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“…have shown that soil texture may affect both GEMMO survival and the proportion between a modified strain and its parent [8,21]. Previous studies on survival of introduced Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Survival of genetically modified Pseudomonas fluorescens introduced into subtropical soil microcosms

Araujo

1994

FEMS Microbiology Ecology

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A genetically modified strain of Pseudomonas fluorescens and its parent showed grossly similar decline rates following introduction into subtropical clay and sandy soils. In unplanted clay soit at pH 6.9 and 25°C, population densities declined progressively from about 108 to 103 colony forming units (cfu) g−1 dry soil over 75 days, but in unplanted sandy soil the introduced populations could not be detected after 25 days. In clay soil at pH 8.7 or 4.7, or at environmental temperature, decay rates were enhanced as compared to those at pH 6.9 and 25°C. Counts of introduced strains in clay bulk soil and in rhizosphere and rhizoplane of maize suggested that the introduced bacteria competed well with the native bacteria, and colonized the roots at about 106 cfu g−1 dry root at 25°C, over 20 days. However, rhizoplane colonization was lower at environmental temperature. The decay rate of both strains was slower in planted than in unplanted sandy soil. The population densities in the rhizosphere and rhizoplane in the sandy soil were significantly lower than those in the clay soil. Both introduced strains colonized the maize roots in both soils, using seeds coated with bacteria in 1% carboxymethyl cellulose. Introduced cells were localized at different sites along the roots of plants developing in clay soil, with higher densities in the original (near the seeds) and root hair zones as compared to the intermediate zones. No significant difference was observed between the extent of root colonization of the genetically modified strain and its parent.

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“…have shown that soil texture may affect both GEMMO survival and the proportion between a modified strain and its parent [8,21]. Previous studies on survival of introduced Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Survival of genetically modified Pseudomonas fluorescens introduced into subtropical soil microcosms

Araujo

1994

FEMS Microbiology Ecology

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“…Among PGPR species, fluorescent pseudomonads are frequently recovered from both the soil and plant rhizospheres. Among these, Pseudomonas fluorescens is a well-known soil and rhizosphere inhabitant (Van Elsas et al 2006) that can tolerate high concentrations of heavy metals in polluted waters (Wasi et al 2010). Its utilisation as a seed bioinoculant for hyperaccumulator plants is currently unknown, and there are very few studies aimed at investigating its capability for increasing the metal availability of the rhizospheres of such plants (Dell'Amico et al 2008;Sheng et al 2008).…”

mentioning

confidence: 99%

Effects of Pseudomonas fluorescens Seed Bioinoculation on Heavy Metal Accumulation for Mirabilis jalapa Phytoextraction in Smelter-Contaminated Soil

Petriccione¹,

Patre²,

Ferrante³

et al. 2013

Water Air Soil Pollut

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Some Pseudomonas fluorescens strains, consistently isolated from the rhizosphere of wild plants grown in a soil that was highly polluted with illegal waste of smelter residues, were utilised for Mirabilis jalapa seed bioinoculation to verify their effects on seed germination and on promoting a higher heavy metal accumulation in the plant rhizosphere and/or uptake in the leaves. The high content of heavy metals in the soil induced a decrease in either the leaf dry weight or photosynthetic pigment concentration during all vegetative phase of M. jalapa. Bioinoculation with P. fluorescens strains significantly increased the germination of seeds and the root length in the contaminated soil. In some bacterial strain/seed combination, bioinoculation significantly increased the accumulation of heavy metals in M. jalapa rhizosphere. For Cd, the concentration of this metal in the rhizospheres of bioinoculated plants ranged from 270 to 910 μg g −1 of dry weight compared with 200 μg g −1 of dry weight for the non-coated plants. Two P. fluorescens strains, AA27 and MO49, which were isolated from Artemisia annua and Melilotus officinalis, respectively, induced a significantly higher rhizosphere availability also for Cr, Cu, Ni and Zn. However, despite the relevant accumulation of the heavy metals in the plant rhizosphere, generally the metal uptake into the leaves was rather low. Both analysis of variance and principal component analysis confirmed this finding. However, one P. fluorescens strain, CD1, which was isolated from the multi-metal accumulator Cynodon dactylon, significantly promoted the M. jalapa leaf uptake for Cr, Cu and Zn. The plant metal uptake assessment, confirmed the per se capability of M. jalapa to effectively uptake Cd (30 %) and Cu (12.72 %) from the rhizosphere to the leaves, whereas the uptake for the other metals was low: Ni (2.66 %), Zn (2.46 %), Cr (1.75 %), Pb (0.73 %).

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“…Another possible explanation is that the costs associated with the beta-galactosidase gene are greater than that of AaIT. van Elsas et al (1991) andDe Leij et al (1998) both showed that the Þtness of different recombinant P. fluorescens strains varied depending on the insert. The cost of the AaIT gene to AcMNPV appears to be small because the insert was not lost in any of 17 replicates following seven serial passages of AcMNPVAaIT OBs in T. ni.…”

Section: Discussionmentioning

confidence: 98%

Recombinant and Wild-Type Nucleopolyhedroviruses are Equally Fit in Mixed Infections

Milks¹,

Leptich²,

Theilmann³

2001

Environ Entomol

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Survival of genetically engineered Pseudomonas fluorescens in soil in competition with the parent strain

Cited by 32 publications

References 20 publications

Survival of genetically modified Pseudomonas fluorescens introduced into subtropical soil microcosms

Survival of genetically modified Pseudomonas fluorescens introduced into subtropical soil microcosms

Effects of Pseudomonas fluorescens Seed Bioinoculation on Heavy Metal Accumulation for Mirabilis jalapa Phytoextraction in Smelter-Contaminated Soil

Recombinant and Wild-Type Nucleopolyhedroviruses are Equally Fit in Mixed Infections

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