Survival of F-Specific RNA Coliphage, Feline Calicivirus, and<i>Escherichia coli</i>in Water: a Comparative Study

Allwood, Paul B.; Malik, Yashpal Singh; Hedberg, Craig W.; Goyal, Sagar M.

doi:10.1128/aem.69.9.5707-5710.2003

Cited by 88 publications

(68 citation statements)

References 39 publications

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“…This acceleration in the inactivation kinetics of F-specific bacteriophages with increasing temperatures is also observed in experiments with laboratory-grown bacteriophages. Thus, McLaughlin and Rose described very high decay rates for MS2, an F-specific bacteriophage, in seawater at 30˝C [110], and Allwood et al revealed that the decay rate of MS2 at 37˝C is even greater than that of E. coli [111]. All these results suggest that the persistence of F-specific RNA phages might be rated as low in waters with temperatures greater than 25˝C.…”

Section: Persistence In Water Environments and Resistance To Treatmentmentioning

confidence: 62%

Coliphages as Model Organisms in the Characterization and Management of Water Resources

Torroella

Gutiérrez

Blanch

et al. 2016

Water

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Two groups of bacteriophages that infect Escherichia coli, somatic and F-specific coliphages, have been used in academia as both fecal and viral indicators for many years. Regulatory authorities in different parts of the world are beginning to consider coliphages as indicators of water quality in a range of settings. However, issues such as their potential replication in natural water environments, the cumbersome detection and enumeration methods, a lack of definition concerning which of the two groups should be included in future regulations, and the lack of a clear correlation between coliphages and human viruses and health risks in different water settings remain controversial. This review attempts to shed some light on these contentious issues. The conclusions are that: 1) supposing that they can replicate in some natural water settings, the contribution of coliphages replicated outside the gut will not affect the numbers contributed by fecal pollution and detected by strains recommended for standardized methods; 2) there are easy, fast, and cost-effective methods that can be used in routine laboratories after a little training; 3) perhaps the best option is to determine both groups in a single step; and 4) the low correlation of coliphages with human viruses and health risks is no worse than the correlation between different human viruses.

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Section: Persistence In Water Environments and Resistance To Treatmentmentioning

confidence: 62%

Coliphages as Model Organisms in the Characterization and Management of Water Resources

Torroella

Gutiérrez

Blanch

et al. 2016

Water

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“…Allwood et al (2003) analysed inoculated potable water with Feline Calicivirus (FCV) at 4, 25 and 378C, during 28 days. They found that an increment in temperature diminished the time required for 90% viral reduction at 7.3, 5.2 and 2 days, respectively.…”

Section: Results and Discussion Presence Of Hav And Nv In Mrwsmentioning

confidence: 99%

“…However, conclusive data remarks negative correlation between Enterococci and viral pathogens (APHA 1999; Griffin et al 1999;Jiang et al 2001;Harwood et al 2005;Moce et al 2005;Vantarakis et al 2005). Viruses are known to persist for a longer time in MRWs (Allwood et al 2003).…”

Section: Information About the Presence Of Enteric Viruses Inmentioning

confidence: 99%

Detection and phylogenetic analysis of hepatitis A virus and norovirus in marine recreational waters of Mexico

León‐Félix

Fernandez

Velarde‐Félix

et al. 2009

Journal of Water and Health

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An investigation was conducted to determine hepatitis A virus (HAV) and norovirus (NV) presence in marine recreational waters (MRWs) from two Mexican tourists beaches (Altata and Mazatlan), located at the northwestern state of Sinaloa, Mexico. Also, Binary Logistic Regression (BLR) analyses were conducted between physicochemical parameters (temperature, turbidity and salinity) and viral organisms (HAV and NV). A total of 32 MRWs samples were collected from April to July of 2006. Samples were processed according to the Environmental Protection Agency (EPA) adsorption-elution method. Overall, 18 MRWs samples (56.3%) were positive for HAV and NV; 4 (22.2%) were obtained from Altata and 14 (77.8%) from Mazatlan. HAV was detected in 3 MRWs samples (9.4%) and NV in 15 samples (46.8%). Phylogenetic analysis showed the presence of genotype I sub genotype B for HAV and NV genogroup II. BLR analysis showed significant correlations between NV and physicochemical parameters (temperature, turbidity and salinity) (p=0.017, p=0.08, p=0.048, respectively). No significant correlation between physicochemical parameters and HAV was observed. The results indicated that MRW quality of Sinaloa beaches is affected by human faecal pollution. Viral surveillance programs should be implemented to minimize health risks to bathers.

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“…Enteric viruses are one of the most important causative agents of waterborne gastroenteritis because of their high infectivity (13,43), persistence in water (1,2), and tolerance to wastewater treatment (9,33) and chlorination (44). Therefore, monitoring viruses in the environment is important for protecting public health (48).…”

mentioning

confidence: 99%

Validation of Internal Controls for Extraction and Amplification of Nucleic Acids from Enteric Viruses in Water Samples

Hata

Katayama

Kitajima

et al. 2011

Appl Environ Microbiol

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Inhibitors that reduce viral nucleic acid extraction efficiency and interfere with cDNA synthesis and/or polymerase activity affect the molecular detection of viruses in aquatic environments. To overcome these significant problems, we developed a methodology for assessing nucleic acid yields and DNA amplification efficiencies for environmental water samples. This involved adding particles of adenovirus type 5 and murine norovirus and newly developed primer-sharing controls, which are amplified with the same primer pairs and result in the same amplicon sizes as the targets, to these samples. We found that nucleic acid loss during the extraction process, rather than reverse transcription-PCR (RT-PCR) inhibition, more significantly attributed to underestimation of the presence of viral genomes in the environmental water samples tested in this study. Our success rate for satisfactorily amplifying viral RNAs and DNAs by RT-PCR was higher than that for obtaining adequate nucleic acid preparations. We found that inhibitory properties were greatest when we used larger sample volumes. A magnetic silica bead-based RNA extraction method effectively removed inhibitors that interfere with viral nucleic acid extraction and RT-PCR. To our knowledge, this is the first study to assess the inhibitory properties of environmental water samples by using both control virus particles and primersharing controls.

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Survival of F-Specific RNA Coliphage, Feline Calicivirus, andEscherichia coliin Water: a Comparative Study

Cited by 88 publications

References 39 publications

Coliphages as Model Organisms in the Characterization and Management of Water Resources

Coliphages as Model Organisms in the Characterization and Management of Water Resources

Detection and phylogenetic analysis of hepatitis A virus and norovirus in marine recreational waters of Mexico

Validation of Internal Controls for Extraction and Amplification of Nucleic Acids from Enteric Viruses in Water Samples

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