Survival of Escherichia coli K12 in seawater

Sørensen, Søren J.

doi:10.1111/j.1574-6941.1991.tb01720.x

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…Marine vibrios maintain viability when the cells encounter prolonged starvation conditions [17,18]. Several studies have also addressed the ability of E. coil cells to survive in sea-water [19,20] and to resist other forms of stress [6]. The survival of E. coli in sea-water seems to be strain dependent and the ability to survive is related to the ability to osmoregulate [21].…”

Section: Discussionmentioning

confidence: 99%

Bacteria starved for prolonged periods develop increased protection against lethal temperatures

Jouper-Jaan¹

1992

FEMS Microbiology Ecology

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Section: Discussionmentioning

confidence: 99%

Bacteria starved for prolonged periods develop increased protection against lethal temperatures

Jouper-Jaan¹

1992

FEMS Microbiology Ecology

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“…A few studies describe the survival of bacteria in small shake flasks with sterile, enriched, or unamended water [17,18,22,29,33,36], in survival chambers [1], and in simple water/sediment microcosms [28]. However, because the microbial ecology of these microcosms is poorly understood, it is extremely difficult to extrapolate data on fate and/or effects of GEMs in these systems to the environment.…”

Section: Introductionmentioning

confidence: 99%

Microbial trophic interactions in aquatic microcosms designed for testing genetically engineered microorganisms: A field comparison

Kroer¹,

Coffin

1992

Microb Ecol

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Microcosms may potentially be used as tools for evaluating the fate and effects of genetically engineered microorganisms released into the environment. Extrapolation of data to the field, however, requires that the correspondence between microcosm and field is known. Microbial trophic interactions within the microbial loop were compared quantitatively and qualitatively between field and microcosms containing estuarine water with and without intact sediment cores. The comparison showed that whereas proportions between trophic levels in microcosms were qualitatively similar to those in the field, rates of microbial processes were from 25 to 40% lower in microcosms. Nitrogen cycling was disrupted in microcosms incubated in the dark to eliminate primary production. Examination of the microbial parameters further suggests that sediment in microcosms may be an important factor regulating the bacterial trophic level. These results demonstrate that analysis of microbial trophic interactions is a sensitive method for the field comparison of aquatic microcosms and a potentially useful tool in the risk assessment of genetically engineered microorganisms.

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“…The survival of enteric bacteria in aquatic environments has been described in terms of temperature (Rhodes & Kator, 1988), solar radiation (Barcina et al, 1986), the presence of toxins (Gurijala & Alexander, 1990), nutrient starvation, osmotic stress (Kjelleberg et al, 1987;Munro et al, 1989;Sorensen, 1991), competition with the autochthonous microflora, predation by protozoa (Garcia-Lara et al, 1991b; 0001-7877 0 1993 SGM Kator, 1988;Sorensen, 1991) and the presence of bacteriophages (Garcia-Lara et al, 1991 b) or lytic microflora (Gurijala & Alexander, 1990). However, allochthonous bacteria react to starvation and stress in aquatic systems in many ways.…”

Section: Introductionmentioning

confidence: 99%

Effect of previous growth conditions on the starvation-survival of Escherichia coli in seawater

García‐Lara

Martínez²,

Vilamú

et al. 1993

Journal of General Microbiology

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The starvation-survival of Escherichia coli in seawater was assessed by plate and epifluorescence counts, 3H-label decrease, cellular DNA concentrations, and metabolic activities. These assays were performed on two types of populations, adapted and non-adapted to seawater. The number of viable cells in the adapted population remained constant throughout starvation-survival in sterile seawater. In contrast, a significant decrease in the ability of the non-adapted E. coli to form colonies on plates following starvation-survival in sterile seawater was observed. However, this drop in viable counts was not mirrored by the epifluorescence counts and 3H-label, which did not show major changes for either population during the experiments, indicating maintenance of the number of cells. In addition, a significant increase in and subsequent maintenance of DNA content and thymidine incorporation was observed for both populations during starvation-survival in sterile seawater. The changes in cell-attached exoproteolytic activity and electron transport system activity showed that adapted and non-adapted E. coli cells maintain their metabolic potential. Cell-free exoproteolytic activity was drastically reduced in both populations. Adapted cells showed higher electron transport system activity and thymidine incorporation than non-adapted cells at the onset of starvation-survival. The effect of previous adaptation on E. coli starvation-survival, as assessed by plate counts and 3H-label decrease, was also observed in raw seawater. It seems from these data that the biological potential of E. coli cells suspended in sterile seawater has not been switched off or impaired seriously.

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Survival of Escherichia coli K12 in seawater

Cited by 3 publications

References 29 publications

Bacteria starved for prolonged periods develop increased protection against lethal temperatures

Bacteria starved for prolonged periods develop increased protection against lethal temperatures

Microbial trophic interactions in aquatic microcosms designed for testing genetically engineered microorganisms: A field comparison

Effect of previous growth conditions on the starvation-survival of Escherichia coli in seawater

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