Survival of Embryos Resulting from Spermatozoa Produced by Mice Exposed to Elevated Ambient Temperature

Burfening, P. J.; Elliott, Donald; Eisen, E. J.; Ulberg, L. C.

doi:10.2527/jas1970.304578x

Cited by 29 publications

(22 citation statements)

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“…Additionally, no embryos developed into blastocysts when sired 21 days after heating. The increase of abnormal embryos in this study was similar to previous results published for the rat at 15 days after heating [18], and with the reduced implantation rate reported in the mouse at 21 days after heating [8]. A model proposed by Haig and Westoby [47] predicts that paternal imprinted genes will promote growth and maternal genes will inhibit growth.…”

Section: Discussionsupporting

confidence: 91%

“…can damage spermatogenesis, which may result in decreased sperm numbers and reduced sperm motility in the ram [5] and boar [6], normal fertilisation, but an increase in embryonic death in the pig [6], mouse [7][8][9], rabbit [10,11], sheep [12][13][14][15][16][17] and rat [18].…”

Section: Introductionmentioning

confidence: 99%

“…Early studies have shown that paternal heat stress reduces fertilisation rates [8] and the in vivo development of preimplantation embryos in the mouse [7]. The extent of the effect could be related to the time after heatstress of the males [8,18].…”

Section: Introductionmentioning

confidence: 99%

“…The extent of the effect could be related to the time after heatstress of the males [8,18]. Setchell et al [18] reported that local heating of the testis between 7 days and 35 days prior to mating reduces the size and weight of litters in the mouse.…”

Section: Introductionmentioning

confidence: 99%

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Effects of paternal heat stress on the in vivo development of preimplantation embryos in the mouse

Zhu

Setchell

2004

Reprod. Nutr. Dev.

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-The objective of this study was to examine the effect of paternal heat stress on the in vivo development of preimplantation embryos in the mouse. Synchronised B6CBF1 female mice were mated either to a control male mouse or to one that had been exposed at 7, 21 or 35 days previously, for 24 h to an ambient temperature of 36 ± 0.3 °C and 66 ± 5.6% relative humidity. Embryos were collected from the oviducts of mice at 14-16 h, 34-39 h or 61-65 h after mating or from the uterus at 85-90 h after mating and their developmental status was evaluated morphologically. The number of cells within blastocysts was also determined using bisbenzimide-propidium iodide staining. Paternal heat stress 7 days before mating reduced the proportion of embryos developing from 4-cell (4-C) to morulae (M), hatched blastocysts, total blastocysts and the number of inner cell mass (ICM) and trophectoderm (TE) cells in the blastocyst. Paternal heat stress 21 days prior to mating reduced the proportion of 2-C and 4-C to M embryos with no embryos developing to blastocysts. There were also increases in the number of 1-C and abnormal embryos recorded at this time. Paternal heat stress 35 days before mating decreased the proportion of 2-C embryos, expanded blastocysts and ICM and TE cells in the blastocyst. These results support previous work demonstrating that both the sperm in the epididymis and germ cells in the testis are susceptible to damage by environmental heat stress, with spermatocytes being the most vulnerable. This study also demonstrates that subtle effects on the male such as a short exposure to elevated environmental temperatures can translate to quite profound paternal impacts on early embryo development.paternal heat stress / in vivo development / mouse preimplantation embryos

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of paternal heat stress on the in vivo development of preimplantation embryos in the mouse

Zhu

Setchell

2004

Reprod. Nutr. Dev.

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“…It is evident, then, that the major portion of this mortality occurred at a later stage of development. This is further indicated by the greater number of non-viable fetuses observed at Day 10, these presumably being induced by embryos expressing a lethal abnormality nearer the time of implantation (Burfening et al, 1970 …”

Section: Incorporation Of [3h]uridine At 54 Hr Gestationmentioning

confidence: 99%

Viability and Survival of Mouse Embryos Following Parental Exposure to High Temperature

Ar¹

1972

Reproduction

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Tests of mutagenesis and reproduction in male rats exposed to 2,450‐MHz (CW) microwaves

Berman

Carter

House

1980

Bioelectromagnetics

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Tests of mutagenesis and reproduction were conducted in male rats which were irradiated by 2,450-MHz, continuous-wave (CW) microwaves, 4 hr/day from day 6 of gestation to 90 days of age at 5 mW/cm2; or 5 hr/day for five days beginning on the 90th day of age at 10 mW/cm2; or 4 hr/day, 5 days/wk for four weeks, beginning on the 90th day of age. During selected weekly periods after treatment, the rats were bred to pairs of untreated, normal female rats that were examined in late pregnancy by means of dominant lethal assay. The reproductive efficiency of these males, as reflected in their breeding, was also examined for changes relating to their microwave experience. No significant evidence of germ-cell mutagenesis was detected when data of microwave-exposed males were compared with those of sham-exposed males, even though there were significant increases in rectal and intra-testicular temperatures at a power density of 28 mW/cm2. Temporary sterility, as indexed by fewer pregnancies, was seen at the highest power density.

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Survival of Embryos Resulting from Spermatozoa Produced by Mice Exposed to Elevated Ambient Temperature

Cited by 29 publications

References 0 publications

Effects of paternal heat stress on the in vivo development of preimplantation embryos in the mouse

Effects of paternal heat stress on the in vivo development of preimplantation embryos in the mouse

Viability and Survival of Mouse Embryos Following Parental Exposure to High Temperature

Tests of mutagenesis and reproduction in male rats exposed to 2,450‐MHz (CW) microwaves

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