Survival of bifidobacteria in the presence of bile salt

Ibrahim, Salam A.; Bezkorovainy, Anatoly

doi:10.1002/jsfa.2740620407

Cited by 53 publications

(38 citation statements)

References 8 publications

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“…However, L. salivarius FC113, FC621, and FC812, and strain FC322 were the least bile-resistant. The broad variation in bile sensitivity is consistent with the findings of many studies (Chateau et al, 1994;Ibrahim and Bezkorovainy, 1993). …”

Section: Identification Of Lab Strainssupporting

confidence: 90%

Screening of Immunostimulatory Probiotic Lactic Acid Bacteria from Chicken Feces as Animal Probiotics

Lee¹,

Lee²,

Lee³

et al. 2010

Korean Journal for Food Science of Animal Resources

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Section: Identification Of Lab Strainssupporting

confidence: 90%

Screening of Immunostimulatory Probiotic Lactic Acid Bacteria from Chicken Feces as Animal Probiotics

Lee¹,

Lee²,

Lee³

et al. 2010

Korean Journal for Food Science of Animal Resources

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“…PSC101 strain would likely survive stomach passage. Bile tolerance is another property that is required for lactobacilli survival in the small intestine, and thus, bacteria play a role in the physiological function of this organ (Byun et al, 1997;Ibrahim and Bezkorovainy, 1993;Park et al, 1998;Shin et al, 1995). Although the growth rates of the four isolates strains were slightly suppressed by bile salts, Lactobacillus sp.…”

Section: Resultsmentioning

confidence: 99%

Selection of Lactobacillus sp. PSC101 that produces active dietary enzymes such as amylase, lipase, phytase and protease in pigs

Kim

Kim²,

Rhee

et al. 2007

J. Gen. Appl. Microbiol.

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This study was carried out to screen lactic acid bacteria that produce active dietary enzymes, such as amylase, lipase, phytase, and protease, using a two-step process in pigs. We isolated a total of 210 and 132 strains of bacteria, grown under aerobic and anaerobic conditions, respectively, in Man Rogosa Sharpe agar containing 0.13% bile after treatment of intestinal samples at pH 3 for 30 min. From fecal samples, a total of 134 aerobic and 111 anaerobic strains were isolated in the same manner. In the second screening test, we selected four strains that produced four dietary enzymes from isolates obtained in the first screening test. Each strain was characterized as lactobacilli based on the following criteria: rod shape, negative for catalase, Gram positive, and lack of acute oral toxicity in mice. Of these four strains, we finally selected Lactobacillus spp. PSC101, which was resistant to pH 3 for 8 h and grew in the presence of 1% bile. In summary, Lactobacillus sp. PSC101 may be a strong probiotic candidate in swine due to its resistance to both acid and bile, its production of dietary enzymes that promote animal growth, and its non-toxic nature in mice.

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“…Genes coding for these proteins, such as groES, groEL, and dnaK, have been shown to be inducible by osmotic and heat shock in Bifidobacterium breve (57,58). Furthermore, sublethal bile concentrations can also trigger a physiological adaptive response in bifidobacteria (7,23,28), being DnaK induced in some species of Bifidobacterium in the presence of bile (49). Marvin-Guy et al (36) recently used matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for rapid identification of bile salt stress-related fingerprints from whole Bifidobacterium lactis cells, but no information was provided about protein identification and function.…”

mentioning

confidence: 99%

Proteomic Analysis of Global Changes in Protein Expression during Bile Salt Exposure of Bifidobacterium longum NCIMB 8809

Sánchez

Champomier‐Vergès²,

Anglade³

et al. 2005

J Bacteriol

177

171

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Adaptation to and tolerance of bile stress are among the main limiting factors to ensure survival of bifidobacteria in the intestinal environment of humans. The effect of bile salts on protein expression patterns of Bifidobacterium longum was examined. Protein pattern comparison of strains grown with or without bile extract allowed us to identify 34 different proteins whose expression was regulated. The majority of these proteins were induced after both a minor (0.6 g liter ؊1 ) and a major (1.2 g liter ؊1 ) exposure to bile. These include general stress response chaperones, proteins involved in transcription and translation and in the metabolism of amino acids and nucleotides, and several enzymes of glycolysis and pyruvate catabolism. Remarkably, xylulose 5-phosphate/fructose 6-phosphate phosphoketolase, the key enzyme of the so-called bifidobacterial shunt, was found to be upregulated, and the activity on fructose 6-phosphate was significantly higher for protein extracts of cells grown in the presence of bile. Changes in the levels of metabolic end products (acetate and lactate) were also detected. These results suggest that bile salts, to which bifidobacteria are naturally exposed, induce a complex physiological response rather than a single event in which proteins from many different functional categories take part. This study has extended our understanding of the molecular mechanism underlying the capacity of intestinal bifidobacteria to tolerate bile.

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Survival of bifidobacteria in the presence of bile salt

Cited by 53 publications

References 8 publications

Screening of Immunostimulatory Probiotic Lactic Acid Bacteria from Chicken Feces as Animal Probiotics

Screening of Immunostimulatory Probiotic Lactic Acid Bacteria from Chicken Feces as Animal Probiotics

Selection of Lactobacillus sp. PSC101 that produces active dietary enzymes such as amylase, lipase, phytase and protease in pigs

Proteomic Analysis of Global Changes in Protein Expression during Bile Salt Exposure of Bifidobacterium longum NCIMB 8809

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