Survival of anopheline eggs and their susceptibility to infection with Metarhizium anisopliae and Beauveria bassiana under laboratory conditions

Luz, Christian; Mnyone, Ladslaus L.; Russell, Tanya L.

doi:10.1007/s00436-011-2318-3

Cited by 14 publications

(14 citation statements)

References 41 publications

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“…Metarhizium anisopliae is a well-known entomopathogenic fungus that frequently occurs in soils, and in laboratory conditions M. anisopliae strain IP 46, which was isolated from a soil sample collected in Central Brazil, can attack all stages of A. aegypti, including the eggs (Silva et al 2004;Luz et al 2007;Santos et al 2009;Leles et al 2010). This fungal species is a promising agent for integrated mosquito control (Mnyone et al 2009;Leles et al 2010;Luz et al 2011), and there is need for a better understanding of its effectiveness and applicability in order to develop specific mycoinsecticides against dengue vectors. Natural or artificial breeding sites with high densities of adult females, eggs, and larvae seem to be suitable for applications of mycoinsecticides.…”

Section: Introductionmentioning

confidence: 99%

Effects of Metarhizium anisopliae conidia mixed with soil against the eggs of Aedes aegypti

2011

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The effectiveness of Metarhizium anisopliae IP 46 conidia mixed with soil was tested against Aedes aegypti eggs. Mycelium and new conidia developed first on eggs between 4.8 and 15 days respectively after incubation of fungus-treated soils at 3.3 × 10(3) up to 3.3 × 10(5) conidia/g soil at 25°C and relative humidities close to saturation. After 15-day incubation, 53.3% of the eggs exposed to soil with 3.3 × 10(5) conidia/g showed external development of mycelium and conidia. Fungus-inoculated soils (but not untreated controls) showed some mycelial growth and sporulation apart from the eggs. Some eggs on treated soils hatched; those larvae died and eventually showed fungal development on their bodies. The cumulative relative eclosion of larvae after submersion of treated eggs in water decreased from 52.2% at 3.3 × 10(3) conidia/g to 25.3% at 3.3 × 10(5) conidia/g. These findings clearly showed that A. aegypti eggs can be infected by M. anisopliae when deposited on fungus-contaminated soil. The effectiveness of M. anisopliae against gravid females, larvae, and also eggs of A. aegypti underscored the possible usefulness of this fungus as a mycoinsecticide, whether naturally occurring or artificially applied, in the breeding sites of this mosquito.

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Section: Introductionmentioning

confidence: 99%

Effects of Metarhizium anisopliae conidia mixed with soil against the eggs of Aedes aegypti

2011

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“…Ovicidal activities by this or other fungi, however, have been demonstrated only recently against Aedes aegypti and Anopheles spp (Luz et al . , ; Leles et al . ).…”

Section: Introductionmentioning

confidence: 99%

“…Larvicidal and adulticidal activity of the entomopathogenic fungus Metarhizium anisopliae is well established against both aedine (Silva et al 2004;Scholte et al 2007;Paula et al 2008Paula et al , 2011Pereira et al 2009;Leles et al 2010) and anopheline mosquitoes (Scholte et al 2004;Mnyone et al 2009Mnyone et al , 2011Bukhari et al 2011). Ovicidal activities by this or other fungi, however, have been demonstrated only recently against Aedes aegypti and Anopheles spp (Luz et al 2007(Luz et al , 2011Leles et al 2012). The broad insecticidal effect of M. anisopliae against both aquatic and terrestrial mosquito stages enhances the interest in this fungus for integrated control of culicid vectors as a means to diminish their transmission of diseases affecting humans and livestock.…”

Section: Introductionmentioning

confidence: 99%

New insights on the effectiveness of Metarhizium anisopliae formulation and application against Aedes aegypti eggs

Sousa

Lobo

Rodrigues

et al. 2013

Lett Appl Microbiol

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Significance and Impact of the Study: The entomopathogenic fungus Metarhizium anisopliae has been extensively studied as a potential agent for biological mosquito control. This study demonstrates the potential of M. anisopliae s.l. strain IP 46, which can infect adults, larvae and eggs of Aedes aegypti, against the eggs of this important vector species by indirect applications of oil-based conidial formulations. AbstractIncreasing needs for innovative control tools against the dengue vector Aedes aegypti have prompted investigations into the development of specific mycoinsecticides. The entomopathogenic fungus Metarhizium anisopliae attacks both larval and adult stages, but its ovicidal activity against A. aegypti is still little explored. This study reports important findings about the effectiveness of conidia formulated in water and oil-in-water emulsions and of direct and indirect application techniques against A. aegypti eggs. The ovicidal activity of M. anisopliae increased with higher conidial concentrations regardless of the application technique, and larvae elimination concentrations were lowest with oil-in-water-formulated conidia (LEC 50 ≤ 4Á8 9 10 3 conidia cm À2 and LEC 90 ≤ 1Á9 9 10 5 conidia cm À2 , respectively). Conidia eventually stimulated larval eclosion. Consequently, the indirect application of oil-based fungal formulations onto substrates where oviposition will later occur appears to be a more efficient means to infect those eggs than the direct fungal application to previously deposited eggs.

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“…Entomopathogenic fungi as antagonists of insect eggs with medical importance only recently gained more interest, especially against mosquito eggs (Luz et al. , ; Santos et al. ; Leles et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of formulated Metarhizium anisopliae on eggs and eclosing nymphs of Triatoma infestans

Rodrigues

Lobo

Fernandes

et al. 2014

J Applied Entomology

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Little is known about the ovicidal effects of fungi that attack nymphs and adults of triatomine vectors. A combined formulation of Metarhizium anisopliae IP 46 conidia prepared with diatomaceous earth (DE) and vegetable oil was tested against eggs of Triatoma infestans. Eggs were highly susceptible to fungal infection at relative humidity close to saturation [>98% relative humidity (RH)] but not at 75% RH regardless of the formulation applied. Susceptibility of eggs decreased with longer post‐ovipositional embryonation periods before treatments. The eventual eclosion of nymphs was best suppressed by application of conidia prepared with DE + oil and at a >98% RH incubation. Moreover, nymphs were less affected by the fungus when exposed for only a 24‐h period after eclosion to a treated surface than individuals that were in constant contact with the conidia. These findings contribute to a better understanding of the potential of M. anisopliae as an agent against all developmental stages of T. infestans.

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Survival of anopheline eggs and their susceptibility to infection with Metarhizium anisopliae and Beauveria bassiana under laboratory conditions

Cited by 14 publications

References 41 publications

Effects of Metarhizium anisopliae conidia mixed with soil against the eggs of Aedes aegypti

Effects of Metarhizium anisopliae conidia mixed with soil against the eggs of Aedes aegypti

New insights on the effectiveness of Metarhizium anisopliae formulation and application against Aedes aegypti eggs

Effect of formulated Metarhizium anisopliae on eggs and eclosing nymphs of Triatoma infestans

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