Survival, growth and fertility of gynogenetic diploids induced in the cyprinid loach, Misgurnus anguillicaudatus

Suzuki, Ryo; Oshiro, Takashi; Nakanishi, Teruyuki

doi:10.1016/0044-8486(85)90051-1

Cited by 89 publications

(40 citation statements)

References 19 publications

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“…Production of sex-reversed clonal males was performed according to the procedure described by Yoshikawa et al [15]. In May 2005, progeny of the clonal diploid loach were obtained by fertilizing eggs of the clonal female with sperm of goldfish (Carassius auratus, Cyprinidae) that had been irradiated with UV rays according to the procedure described by Suzuki et al [16]. Clonal larvae were fed carp feed containing 2.5 ppm of 17a-methyltestosterone (MT; Wako, Osaka, Japan) for 30 days initiated 1 mo after hatching.…”

Section: Fish Specimensmentioning

confidence: 99%

Chromosome Doubling in Early Spermatogonia Produces Diploid Spermatozoa in a Natural Clonal Fish1

Yoshikawa

Morishima

Fujimoto

et al. 2009

Biology of Reproduction

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The natural clonal loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) is diploid (2n ¼ 50) and produces genetically identical unreduced eggs, which develop into diploid individuals without any genetic contribution from sperm. Artificially sexreversed clones created by the administration of 17alpha-methyltestosterone produce clonal diploid sperm. In metaphase spreads from testicular cells of the sex-reversed clones, spermatocytes had twice the normal number of chromosomes (50 bivalents) compared with those of normal diploids (25 bivalents). Thus, the production of unreduced diploid spermatozoa is initiated by premeiotic endomitosis (or endoreduplication), chromosome doubling before meiosis, and is followed by two quasinormal divisions. Larger nuclei in the germ cells were observed in all stages of type B spermatogonia in the testes of the sex-reversed clones. In contrast, besides having larger type A spermatogonia, the sex-reversed clones also had the type A spermatogonia that were the same size as those of normal diploids. It follows that chromosome duplication causing unreduced spermatogenesis occurred in the type A spermatogonia. The presence of tetraploid type A and early type B spermatogonia, identified by labeling with antispermatogoniaspecific antigen 1, was verified using DNA content flow cytometry. These results support the conclusion that chromosome doubling occurs at the type A spermatogonial stage in diploid spermatogenesis in the clonal fish.

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Section: Fish Specimensmentioning

confidence: 99%

Chromosome Doubling in Early Spermatogonia Produces Diploid Spermatozoa in a Natural Clonal Fish1

Yoshikawa

Morishima

Fujimoto

et al. 2009

Biology of Reproduction

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“…The diploid members of the clonal lineage must be all-female because sex-determination of the loach is estimated as male heterogamety with the XX female-XY male system (Suzuki et al, 1985) …”

Section: Diploid Sperm Of Diploid-triploid Mosaic Loachesmentioning

confidence: 99%

Ploidy manipulation using diploid sperm in the loach,Misgurnus anguillicaudatus: a review

Yoshikawa

Morishima

Fujimoto

et al. 2008

Journal of Applied Ichthyology

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SummaryIn the loach, Misgurnus anguillicaudatus (Teleoste: Cobitidae), diploid sperm can be obtained from natural tetraploid individuals with four sets of homologous chromosomes. Using diploid sperm, various kinds of polyploids and androgenetic diploids have been produced. Cryptic clonal lineages are also recognized in wild populations of the loach. They produce unreduced diploid eggs genetically identical to somatic cells of the mother fish and most diploid eggs develop gynogenetically as a member of the clone. However, some eggs develop to triploid and/or diploid-triploid mosaic individuals by incorporation of sperm nucleus. Diploid-triploid mosaic males exclusively generate fertile diploid sperm with clonal genotypes. Such diploid sperm can also be obtained from artificially sex-reversed clonal individuals. Recent population studies suggested that Japanese M. anguillicaudatus might not be a single species, but a complex involving cryptic species, because wild populations exhibited genetic differentiation at interspecific level. This implies possible relationship between atypical reproduction and natural hybridization in the loach.

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“…Some eggs from each female were fertilized with sperm from one of the four male loaches. The remainder of each female eggs was fertilized with ultraviolet ray (UV) irradiated sperm of the goldfish in order to induce gynogenetic development, following the procedure described by Suzuki et al (1985). Female No.…”

Section: Induced Gynogenesis and Interspecific Hybridizationmentioning

confidence: 99%

A Cryptic Clonal Line of the Loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) Evidenced by Induced Gynogenesis, Interspecific Hybridization, Microsatellite Genotyping and Multilocus DNA Fingerprinting

et al. 2002

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-In Memanbetsu town, Hokkaido island, Japan, a high frequency of natural triploid loaches Misgurnus anguillicaudatus (7.4% on average) was detected by flow cytometry for relative DNA content. Among sympatric diploid females (n=6) from a single population, we found two unique females that laid unreduced diploid eggs. They gave normal diploid progeny even after induction of gynogenesis with genetically inert UV-irradiated sperm. When fertilized with normal loach sperm, some unreduced eggs developed into triploids, but the rest into diploids. Hybridization using goldfish Carassius auratus sperm gave both normal diploid loaches and inviable allotriploid hybrids possessing the diploid loach genome and the haploid goldfish genome. Microsatellite genotyping and DNA fingerprinting demonstrated that the diploid progeny developing from the unreduced eggs were genetically identical to the mother, while the triploids had some of the paternal DNA. These results indicate that the diploid eggs reproduced unisexually as a diploid clone and in other cases developed into triploids after accidental incorporation of sperm nucleus. The presence of at least one clonal line in this area was shown by the identical DNA fingerprint detected in five out of 17 diploid loaches examined.

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Survival, growth and fertility of gynogenetic diploids induced in the cyprinid loach, Misgurnus anguillicaudatus

Cited by 89 publications

References 19 publications

Chromosome Doubling in Early Spermatogonia Produces Diploid Spermatozoa in a Natural Clonal Fish1

Chromosome Doubling in Early Spermatogonia Produces Diploid Spermatozoa in a Natural Clonal Fish1

Ploidy manipulation using diploid sperm in the loach,Misgurnus anguillicaudatus: a review

A Cryptic Clonal Line of the Loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) Evidenced by Induced Gynogenesis, Interspecific Hybridization, Microsatellite Genotyping and Multilocus DNA Fingerprinting

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