Survival, distribution, and gene transfer of bacteria in a compact soil microcosm system

Henschke, R. B.; Schmidt, Fr.

doi:10.1007/bf00260511

Cited by 39 publications

(25 citation statements)

References 22 publications

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“…Before use the soil was sieved. The microcosms used in this study consisted of a soil column as described previously (Henschke and Schmidt 1989). Sieved soil (10-12 g; mesh size 2 mm) was packed in each glass column.…”

Section: Introductionmentioning

confidence: 99%

“…Sieved soil (10-12 g; mesh size 2 mm) was packed in each glass column. All experiments were performed at 20 ° C. Inoculation of soil microcosms was done as described by Henschke and Schmidt (1989). Before inoculation cells were grown in NI media containing antibiotics (Ap) to late log phase.…”

Section: Introductionmentioning

confidence: 99%

“…Analysis of the soil was done as described by Henschke and Schmidt (1989). Bacteria were identified by standard techniques and by the API 20NE system, API System (bioMerieux), Montalieu Vercieu, France.…”

Section: Introductionmentioning

confidence: 99%

“…All experiments were performed at least twice. The given data were derived from plating five to six different dilutions of the soil extracts (Henschke and Schmidt 1989) with two plates for each dilution. Soil extracts were plated on Nil agar (giving the total number of viable cfu) and Ap-agar giving the number of bacteria with a resistance to Ap.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain

Henschke¹,

Henschke²,

Schmidt³

1991

Appl Microbiol Biotechnol

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A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using different monitoring methods, including the polymerase-chain reaction, to amplify and detect the specific rDNA. Depending on nutritional status, both the GEM and its rDNA had disappeared within 16 (amended soil) or 28 days (non-amended soil) with a limit of detection of 5 cells/g soil and 20 fg DNA/g soil.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain

Henschke¹,

Henschke²,

Schmidt³

1991

Appl Microbiol Biotechnol

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“…Gene transfer has been repeatedly shown to occur in soil by using introduced donors and recipients [1][2][3][4][5][6][7][8][9]. A limited number of studies have demonstrated gene transfer from an intro-106 duced GEM into the indigenous microbial population.…”

Section: Introductionmentioning

confidence: 99%

Leaching of Pseudomonas aeruginosa, and transconjugants containing pR68.45 through unsaturated, intact soil columns

Lovins¹,

Angle²,

Wiebers³

et al. 1993

FEMS Microbiology Ecology

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Leaching of genetically engineered microbes (GEMs) through soil is a significant concern related to groundwater quality.The objective of this study was to examine the leaching, survival and gene transfer of a genetically engineered microbe and indigenous recipients of pR68.45 in nonsterile, undisturbed soil columns. Pseudomonas aeruginosa PAO25, containing the plasmid R68.45, was added to the surface of undisturbed soil columns (10 em diameter x 80 cm length). Unsaturated flow conditions were maintained by 100 ml daily additions of 2 mM CaCI2 for a period of 70 days. The population of the GEM exhibited a significant (P = 0.05) linear decline with time. The GEM leached only to a depth of 30-40 cm in 70 days. Transfer of pR68.45 was shown to occur from P. aeruginosa into the indigenous bacterial population although relatively low numbers of transconjugants were observed (log 2 cfu g-1 dry soil). The number of transconjugants also decreased with depth and time. Leaching of transconjugants, however, occurred more readily than that of the GEM, probably as a result of ptasmid transfer into smaller, more mobile bacteria. At 70 days incubation, no GEMs were detected in the columns, while transconjugants were observed at several depths. These results demonstrate the importance of examining both the survival and movement of GEMs and transconjugants in soil.

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Dispersal, heterogeneity and resistance: challenging soil quality assessment

Bengtsson¹

1997

Ecological Risk Assessment of Contaminants in Soil

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Survival, distribution, and gene transfer of bacteria in a compact soil microcosm system

Cited by 39 publications

References 22 publications

Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain

Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain

Leaching of Pseudomonas aeruginosa, and transconjugants containing pR68.45 through unsaturated, intact soil columns

Dispersal, heterogeneity and resistance: challenging soil quality assessment

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