Survival curves for microbial species stored by freeze-drying

Miyamoto‐Shinohara, Yukie; Sukenobe, Junji; Imaizumi, Tsutomu; Nakahara, Toro

doi:10.1016/j.cryobiol.2005.09.002

Cited by 62 publications

(32 citation statements)

References 29 publications

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“…The ampoules in these previous studies were sealed under a high vacuum. We described how our freeze-dried bacteria sealed in ampoules at 1 Pa lived longer than those sealed at approximately 7 Pa (Antheunisse, 1973); survival duration was positively correlated with vacuum pressure (Antheunisse, 1973;Miyamoto-Shinohara et al, 2006). In this study, we investigated the effect of vacuum pressure and storage temperature on the survival rates of various yeast strains.…”

Section: Introductionmentioning

confidence: 99%

Survival of yeasts stored after freeze-drying or liquid-drying

Miyamoto‐Shinohara¹,

Nozawa²,

Sukenobe³

et al. 2010

J. Gen. Appl. Microbiol.

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IntroductionFreeze-drying and L-drying are popular methods of preserving microorganisms, because long-term viability is excellent in most cases and the storage and distribution requirements are simple. By plotting the survival curves of freeze-dried species sealed under high vacuum ( 1 Pa) and stored at 5 C in the dark, we previously found that the survival rates of Gram-positive bacteria immediately after freeze-drying and during storage were higher than those of Gram-negative bacteria (Miyamoto-Shinohara et al., 2008). In addition, the survival rate of the yeast Saccharomyces cerevisiae immediately after freeze-drying was lower than those of bacterial species, although S. cerevisiae survival rates were stable during storage (Miyamoto-Shinohara et al., 2000.To increase the survival rates of S. cerevisiae strains, we tested the applicability of L-drying, in which cells are desiccated by vacuum evaporation at room temperature. In contrast, during freeze-drying, cells are desiccated by sublimation. L-drying is effective for the long-term preservation of cultures sensitive to freezedrying (Malik, 1990(Malik, , 1999. In this research, yeast strains were L-dried according to the method of the Institute for Fermentation, Osaka (Banno et al., 1979), in which the cell suspension was dispensed into a glass ampoule (in the same way as for freeze-drying) and a cotton wool plug was inserted to prevent the temperature in the ampoule from falling to the freezing point (Iijima and Sakane, 1973).To clarify the mechanisms underlying tolerance to L-drying and freeze-drying, we investigated the survival of type strains of various yeast species. The survival J. Gen. Appl. Microbiol., 56, 107 119 (2010) We investigated

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Section: Introductionmentioning

confidence: 99%

Survival of yeasts stored after freeze-drying or liquid-drying

Miyamoto‐Shinohara¹,

Nozawa²,

Sukenobe³

et al. 2010

J. Gen. Appl. Microbiol.

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“…The yield of the freeze-drying process has been reported to be lower when using Vibrio species than the yield obtained with other bacterial groups [11]. The cell survival rates and luminescence maintenance were enhanced in the industrial preparations over our laboratory-scale tests.…”

Section: Discussionmentioning

confidence: 91%

Optimal conditions for stability of photoemission and freeze drying of two luminescent bacteria for use in a biosensor

Camanzi¹,

Bolelli²,

Maiolini³

et al. 2011

Enviro Toxic and Chemistry

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Bioluminescent bacteria have been used for many years for biotoxicological analysis. One of the main concerns with this microorganism is the low experimental repeatability when subjected to external factors. The aim of the present study was to obtain accurate, sensitive, and repeatable measurements with stable signals (during the detection and over days) for application in a water-analysis device for the detection of pollutants. Growth conditions were tested and optimized. An optimal freeze-drying procedure for the constitutive bioluminescent bacteria Vibrio fischeri and Photobacterium phosphoreum was developed. The luminescence stability after rehydration was also investigated. Freeze drying was found to be a critical process in survival and signal stability of luminescent bacteria; for this reason, different suspension fluids and various bacterial pellet/suspension fluid ratios (g/ml) were evaluated. The toxicity of heavy metals and organic compounds in water was determined to investigate the applicability of a test based on bacteria obtained in this way, comparing the data with legal limits. A scale-up process was developed with industrial technology: freeze-dried bacteria that emitted a stable luminous signal after rehydration were obtained. Moreover, the median effective concentration (EC50) was calculated with these bacteria.

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“…2C) may emerge as the most economical class of vaccines for mass immunization. Its potency can potentially be further improved by increasing the survival rate of E. coli cells during lyophilization as described previously (25).…”

Section: Discussionmentioning

confidence: 99%

“…Viable E. coli cells can be stored at ambient temperatures as lyophilized powder (23,25), and therefore, E. coli-vectored vaccines can be stockpiled and shipped without reliance on an unbroken cold chain. It is promising that the lyophilized-irradiated E. coli-vectored epicutaneous vaccine (Fig.…”

Section: Discussionmentioning

confidence: 99%

Topical Application of Escherichia coli -Vectored Vaccine as a Simple Method for Eliciting Protective Immunity

Zhang¹,

Shi²,

Kong³

et al. 2006

Infect Immun

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We report here that animals can be protected against lethal infection by Clostridium tetani cells and Bacillus anthracis spores following topical application of intact particles of live or ␥-irradiated Escherichia coli vectors overproducing tetanus and anthrax antigens, respectively. Cutaneous ␥␦T cells were rapidly recruited to the administration site. Live E. coli cells were not found in nonskin tissues after topical application, although fragments of E. coli DNA were disseminated transiently. Evidence suggested that intact E. coli particles in the outer layer of skin may be disrupted by a ␥␦T-cell-mediated innate defense mechanism, followed by the presentation of E. coli ligand-adjuvanted intravector antigens to the immune system and rapid degradation of E. coli components. The nonreplicating E. coli vector overproducing an exogenous immunogen may foster the development of a new generation of vaccines that can be manufactured rapidly and administered noninvasively in a wide variety of disease settings.Noninvasive epicutaneous vaccination without pain, fear, and tissue damage (35, 38) offers distinct advantages over conventional vaccination regimens in that it can be administered by nonmedical personnel and potentially has a higher compliance rate. Administration of vaccines to the surface of skin may also trigger efficient antigen presentation, as the outer layer of skin is more immunocompetent than deep tissue (9, 29). To date, both animals and humans have been immunized against a wide variety of antigens and pathogens by topical application of adenovirus-vectored vaccines (4,17,22,29,35,38) and bacterial toxin-adjuvanted proteins (11-13).To counteract unpredicted disease outbreaks and bioterrorist attacks, vaccines have to be not only safe and efficacious but also amenable to rapid, large-scale production. The Escherichia coli bacterium is fully defined at the molecular level (3) and has proven to be a simple and efficient vector system for the production of exogenous proteins since its first use, which marked the advent of the recombinant DNA era (1, 19). Recombinant plasmid DNA isolated from transformed E. coli vectors is also effective in eliciting an immune response when used as a genetic vaccine (33, 37). We report here that there is no need to biochemically purify recombinant protein or DNA as a vaccine from E. coli vectors. Topical application of intact E. coli particles overproducing pathogen-derived antigens can effectively mobilize the immune repertoire toward beneficial immune protection against relevant pathogens through the controlled activation of an E. coli-targeted defense mechanism in the outer layer of skin. Production and administration of this new class of vaccines are less dependent upon medical resources than any other vaccination regimens. Moreover, this demonstration provides compelling evidence that a cutaneous defense mechanism that rapidly disrupts invading bacterial cells indeed exists along the precarious skin barrier to ward off infections. MATERIALS AND METHODSRecombinant E. c...

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Survival curves for microbial species stored by freeze-drying

Cited by 62 publications

References 29 publications

Survival of yeasts stored after freeze-drying or liquid-drying

Survival of yeasts stored after freeze-drying or liquid-drying

Optimal conditions for stability of photoemission and freeze drying of two luminescent bacteria for use in a biosensor

Topical Application of Escherichia coli -Vectored Vaccine as a Simple Method for Eliciting Protective Immunity

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