Survival and Immunogenicity of Mesenchymal Stem Cells From the Green Fluorescent Protein Transgenic Rat in the Adult Rat Brain

Moloney, Teresa C.; Dockery, Peter; Windebank, Anthony J.; Barry, Frank; Howard, Linda; Dowd, Eilís

doi:10.1177/1545968309357745

Cited by 43 publications

(54 citation statements)

References 55 publications

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“…It is notable that kinetic analysis of 11 C-CFT binding and histology results suggested that at least a portion of MSC-DP cells survived in situ for over 9 months. This is in contrast to naive MSCs, which have much shorter survival times in the brain and disappeared within 3 months in rodent studies; one possible reason for this could be senescent or apoptotic changes (12,13). The long-term survival of MSC-DP cells may have been accompanied by gradual integration into or reinnervation of the host striatal tissues.…”

Section: Figurementioning

confidence: 80%

“…The rate of 11 C-CFT binding reduction, calculated based on the 1-hit model of neurodegeneration (32), was 0.30 months (~10 days) as a half-life period ( Figure 3E). This rate of reduction was slightly slower than that for engrafted naive rodent MSCs (~3 days; Supplemental Figure 2) based on previously described data (13). We further tested whether this degenerative process would affect all grafted cells.…”

Section: C-cft Petmentioning

confidence: 99%

“…But more highly differentiated DA neurons recently engineered from ES cells showed good survival (47). Undifferentiated MSCs are known to die very rapidly, within several days after being engrafted into the brain, and this seems to be due to facilitated senescence (12) or programmed cell death of the MSCs (13). Therefore, we have followed the working hypothesis, testing well-differentiated cells derived from MSCs.…”

Section: Figurementioning

confidence: 99%

“…13. The actual equation for the model was VG = V0 × exp(−ln [2] × t/T1/2) + Vp, where the VG is the time course of graft BPND or volume; V0 is the initial value for VG; exp is the exponential function; t is the time after grafting; T1/2 is a half-life period; and Vp is plateau.…”

Section: Evaluation Of Msc-dp Cells -Rt-pcrmentioning

confidence: 99%

“…The MSCs have been already tested for cell therapy in PD model rodents (7)(8)(9) and even in patients with PD (10). However, they have shown poor performance for restoration of motor function, potentially due to limited spontaneous differentiation (11) or facilitated apoptosis (12,13) of MSCs. Recent studies of fetal midbrain graft have suggested that better outcomes could be obtained if the graft consisted of well-differentiated A9 dopaminergic neurons (14)(15)(16), the most severely damaged neuronal type in PD (17).…”

Section: Introductionmentioning

confidence: 99%

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Autologous mesenchymal stem cell–derived dopaminergic neurons function in parkinsonian macaques

et al. 2012

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Section: Figurementioning

confidence: 80%

Section: C-cft Petmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Evaluation Of Msc-dp Cells -Rt-pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Autologous mesenchymal stem cell–derived dopaminergic neurons function in parkinsonian macaques

et al. 2012

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Inefficiency in macromolecular transport of SCS‐based microcapsules affects viability of primary human mesenchymal stem cells but not of immortalized cells

Sanz-Nogués

Horan²,

Thompson

et al. 2015

J Biomedical Materials Res

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Microcapsules made of sodium cellulose sulphate (SCS) and poly-diallyl-dimethyl-ammonium chloride (pDADMAC) have been employed to encapsulate a wide range of established cell lines for several applications. However, little is known about the encapsulation of primary cells including human mesenchymal stem cells (hMSCs). Human MSCs are of interest in regenerative medicine applications due to pro-angiogenic, anti-inflammatory and immunomodulatory properties, which result from paracrine effects of this cell type. In the present work we have encapsulated primary hMSCs and hMSC-TERT immortalized cells and compared their behavior and in vitro angiogenic potential. We found that, although both cell types were able to secret angiogenic factors such as VEGF, there was a marked reduction of primary hMSC viability compared to hMSC-TERT cells when cultured in these microcapsules. Moreover, this applied to other primary cell cultures such as primary human fibroblasts but not to other cell lines such as human embryonic kidney 293 (HEK293) cells. We found that the microcapsule membrane had a molecular weight cut-off below a critical size, which caused impairment in the diffusion of essential nutrients and had a more detrimental effect on the viability of primary cell cultures compared to cell lines and immortalized cells.

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Kinetics of thermally induced heat shock protein 27 and 70 expression by bone marrow‐derived mesenchymal stem cells

et al. 2012

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Although bone marrow-derived mesenchymal stem cells (MSCs) are an attractive cell therapy candidate, their potential is limited by poor survival following transplantation. Overexpression of anti-apoptotic heat shock proteins using viral vectors can improve the survival of these cells under stressful conditions in vitro and in vivo. It is also possible to induce heat shock protein expression in many cell types by simply exposing them to a transient, nonlethal elevation in temperature. The response profile of MSCs to such a thermal stress has not yet been reported. Therefore, this study sought to determine the kinetics of thermally induced heat shock protein expression by MSCs in vitro. To determine if heat shock protein expression was a function of thermal stress exposure time, MSCs were exposed to 42°C for 15, 30, 45, and 60 min and were harvested 24 h later. To establish the time-course of heat shock protein expression, MSCs were heat shocked for 60 min and harvested 2, 24, 48, 72, 96, and 120 h later. The cells were then analyzed for Hsp27 and Hsp70 expression by Western blot. Densitometric analysis revealed that exposure to a thermal stress induced expression of both Hsp27 and Hsp70 and that the level of expression was dependant on stress exposure time. Following 60 min of heat stress, both Hsp27 and Hsp70 accumulated maximal expression after 48 h with both proteins returning to constitutive expression levels by 120 h. This study demonstrates that heat shock protein expression can be induced in MSCs by a simple thermal stress.

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Survival and Immunogenicity of Mesenchymal Stem Cells From the Green Fluorescent Protein Transgenic Rat in the Adult Rat Brain

Cited by 43 publications

References 55 publications

Autologous mesenchymal stem cell–derived dopaminergic neurons function in parkinsonian macaques

Autologous mesenchymal stem cell–derived dopaminergic neurons function in parkinsonian macaques

Inefficiency in macromolecular transport of SCS‐based microcapsules affects viability of primary human mesenchymal stem cells but not of immortalized cells

Kinetics of thermally induced heat shock protein 27 and 70 expression by bone marrow‐derived mesenchymal stem cells

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