Survival and DNA repair in ultraviolet-irradiated haploid and diploid cultured frog cells

Freed, Jerome J.; Hoess, Ronald H.; Angelosanto, Frank A.; Massey, Holman C.

doi:10.1016/0027-5107(79)90088-5

Cited by 21 publications

(4 citation statements)

References 31 publications

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“…Chromatographic methods even contributed to the identification of xeroderma pigmentosum as a DNA repair disease . Studies were also made in plants , yeast and frog cells . All concluded to a rather slow NER of CPDs, often referred to as “dark repair” in these organisms, which also possess photoactivated repair pathways or “light repair.”…”

Section: Nucleotide Excision Repairmentioning

confidence: 99%

Insight in DNA Repair of UV‐induced Pyrimidine Dimers by Chromatographic Methods

Douki

Koschembahr

Cadet

2017

Photochem & Photobiology

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UV-induced formation of pyrimidine dimers in DNA is a major deleterious event in both eukaryotic and prokaryotic cells. Accumulation of cyclobutane pyrimidine dimers and pyrimidine (6-4) pyrimidone photoproducts can lead to cell death or be at the origin of mutations. In skin, UV induction of DNA damage is a major initiating event in tumorigenesis. To counteract these deleterious effects, all cell types possess DNA repair machinery, such as nucleotide excision repair and, in some cell types, direct reversion. Different analytical approaches were used to assess the efficiency of repair and decipher the enzymatic mechanisms. We presently review the information provided by chromatographic methods, which are complementary to biochemical assays, such as immunological detection and electrophoresis-based techniques. Chromatographic assays are interesting in their ability to provide quantitative data on a wide range of damage and are also valuable tools for the identification of repair intermediates.

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Section: Nucleotide Excision Repairmentioning

confidence: 99%

Insight in DNA Repair of UV‐induced Pyrimidine Dimers by Chromatographic Methods

Douki

Koschembahr

Cadet

2017

Photochem & Photobiology

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“…In addition, the ICR 2A cells were found to be nearly totally deficient in excision repair so that studies examining DNA synthesis in UV irradiated cells could be carried out over long periods of time without the complicating factor of loss of dimers from DNA. The results of experiments described in this paper suggest that the recovery of normal nascent DNA synthesis may play an important role in cellular survival and may also be associated with the enhancement in PRR observed in a split dose experiment. An additional aspect of the ICR 2A cells employed in these experiments was their proficiency in photoreactivation (Rosenstein and Ohlsson-Wilhelm, 1979;Freed et al, 1979). Because of the specificity of photoreactivating enzyme (DNA photolyase, E.C.…”

Section: Introductionmentioning

confidence: 99%

DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis

Rosenstein¹,

Setlow²

1980

Biophysical Journal

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The ability of ICR 2A frog cells to repair DNA damage induced by ultraviolet irradiation was examined. These cells are capable of photoreactivation but are nearly totally deficient in excision repair. They have the ability to convert the small molecule weight DNA made after irradiation into large molecules but do not show an enhancement in this process when the UV dose is delivered in two separate exposures separated by a 3- or 24-h incubation. Total DNA synthesis is depressed and low molecular weight DNA continues to be synthesized during pulse-labeling as long as 48 h after irradiation. The effects of pyrimidine dimer removal through exposure of UV irradiated cells to photoreactivating light indicate that dimers act as the critical lesions blocking DNA synthesis.

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“…give significantly better fit than if the same values were used for both populations. Significance is tested by the sampled permutation procedure described in the attached reprint (Freed et al, 1979). ..…”

Section: Statistical Evaluation Methodsmentioning

confidence: 99%

“…Images are produced from the best available original document. The properties of our haploid frog cell line with regard to single cell survival after UV and the modes of DNA repair that they express have been described (Freed et al, 1979). These cells readily photoreverse pyrimidine dimers, but excision does not appear to provide a quantitatively important pathway since few dimers are lost during incubation in the dark and ''tmscheduled DNA synthesis" is saturated at low doses.…”

Section: Disclaimermentioning

confidence: 99%

Cellular heredity in haploid cultures of somatic cells. Progress report, August 1978-September 1979. [ICR 2A celline (frogs)]

Freed¹

1979

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Survival and DNA repair in ultraviolet-irradiated haploid and diploid cultured frog cells

Cited by 21 publications

References 31 publications

Insight in DNA Repair of UV‐induced Pyrimidine Dimers by Chromatographic Methods

Insight in DNA Repair of UV‐induced Pyrimidine Dimers by Chromatographic Methods

DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis

Cellular heredity in haploid cultures of somatic cells. Progress report, August 1978-September 1979. [ICR 2A celline (frogs)]

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