Survival and activity of individual bioaugmentation strains

Dueholm, Morten Simonsen; Marques, Irina G.; Karst, Søren Michael; D’Imperio, Seth; Tale, Vaibhav P.; Lewis, Derrick L.; Nielsen, Per Halkjær; Nielsen, Jeppe Lund

doi:10.1016/j.biortech.2015.02.111

Cited by 56 publications

(17 citation statements)

References 23 publications

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“…The survival and activity of individual bioaugmentation had not been monitored in several previous applications of the fungal bioaugmentation for enhanced degradation of environmental pollutants [61][62][63]. We deduce that the slow growth of P.…”

Section: P Chrysosporium Is Affiliated With the Family Phanerochaetamentioning

confidence: 87%

Metagenomic insight into the bioaugmentation mechanism of Phanerochaete chrysosporium in an activated sludge system treating coking wastewater

Wang

et al. 2017

Journal of Hazardous Materials

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Phanerochaete chrysosporium was seeded to a sequencing batch reactor treating phenol wastewater. Compared to the contrast reactor (R1), the bioaugmented reactor (R2) exhibits better performance in sludge settling ability, as well as biomass and phenol removal, even though the added fungus is not persistently surviving in the reactor. Bioaugmentation improved bacterial population, growing up to 10,000 times higher than that of eukaryotes. Metagenomic sequencing results show the bioaugmentation finally increases bacterial and eukaryotic richness, but reduces their community diversity. In contrast to R1, bacterial distribution in R2 is more concentrated in Proteobacteria. The relative abundances of filamentous fungi, yeast and microalgae in R2 are all higher than those in R1 at different treatment phases, and two reactors are finally dominated by different protozoan and metazoan. In conclusion, P. chrysosporium improves reactor performances by influencing microbial community structure, and this phenomenon might be attributed to the ecological competition in sludge and toxicity reduction of phenol wastewater. The novelty of this study emphasizes why a species which is not persistently active in bioreactor still plays a crucial role in enhancing reactor performance. Results obtained here impact the conventional criteria for selection of bioaugmentation microbes used in activated sludge systems.

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Section: P Chrysosporium Is Affiliated With the Family Phanerochaetamentioning

confidence: 87%

Metagenomic insight into the bioaugmentation mechanism of Phanerochaete chrysosporium in an activated sludge system treating coking wastewater

Wang

et al. 2017

Journal of Hazardous Materials

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“…The assay was subsequently used to evaluate the persistence of the strain in activated sludge. (Dueholm et al, 2015) Nitrification (HP) A qPCR assay that targets part of the ammonia-monooxygenase sub-unit alpha gene (amoA), which is a key enzyme in ammonia oxidation by ammonia-oxidizing bacteria (AOB), was developed and used to estimate the population size of AOB in soil samples. (Okano et al, 2004) Nitrate reduction (SG) qPCR assays that targets membrane-bound (narG) and periplasmic (napA) protobacterial nitrate reductases were developed and used to determine their relative abundance in various environments.…”

Section: Methodsmentioning

confidence: 99%

“…A strain-specific qPCR assay can be developed based on unique genomic sequences in the bioaugmentation strains. The abundance of the strains can subsequently be determined using DNA extracted from activated sludge at various time points after the addition of the bioaugmentation strain (Dueholm et al, 2015). Here we show an example of how qPCR has been used to evaluate the persistence of the bioaugmentation strains Pseudomonas monteilii SB3078 and SB3101, which are used for the degradation of aromatic hydrocarbons (Dueholm et al, 2014;.…”

Section: Examplementioning

confidence: 99%

“…qPCR is also a useful tool for determining the fate of individual bioaugmentation strains. This can be done using primers that target unique genomic regions (Dueholm et al, 2015). qPCR may furthermore be used to track the spread of antibiotic resistance genes (Volkmann et al, 2004) and infectious viruses (Kitajima et al, 2014).…”

Section: Optimised Dna Extraction From Wastewater Activated Sludgementioning

confidence: 99%

“…50 mL of effluent water was then removed and replaced by 50 mL of primarily settled wastewater, simulating a hydraulic retention time of 24 h. Samples for DNA extraction were collected and benzene reintroduced to 10 µg mL -1 . The flasks were then sealed and the incubations continued (Dueholm et al, 2015). DNA was essentially extracted as described in Section 8.2.…”

Section: Samplesmentioning

confidence: 99%

See 2 more Smart Citations

Experimental Methods in Wastewater Treatment

Loosdrecht¹,

Nielsen²,

López-Vázquez³

et al. 2016

Water Intelligence Online

137

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PrefaceWastewater treatment is a core technology for water resources protection and reuse, as is clearly demonstrated by the great success of its consequent implementation in many countries worldwide. During the last decennia scientific research has made vast progress in understanding the complex and interdisciplinary aspects of the biological, biochemical, chemical and mechanical processes involved. It can be concluded that the global application of existing knowledge and experience in wastewater treatment technology will represent a cornerstone in future water management, as expressed in the Strategic Development Goals accepted by the UN in September 2015.Only about one fifth of the wastewater produced globally is currently being adequately treated. To achieve the goal for sustainable water management by 2030 would require extra wastewater treatment facilities for about 600,000 people each day. I am convinced that this book will make its own significant contribution to meeting this ambitious goal.In the near future, most of the global population will live in cities and in low and middle-income countries, where most wastewater is not adequately treated. Probably the most limiting factor in achieving the goals for sustainable water management is the lack of qualified, well-trained professionals, able to comprehend the scientific research results and transfer them into practice. It is therefore of prime importance to make currently available scientific advances and proven experiences in wastewater treatment technology applications easily accessible worldwide. This was one of the drivers for the development of this book, which represents an innovative contribution to help overcome such a capacity development challenge. The book is most definitely expected to contribute to bridging the gaps between the science and technology, and their practical applications.The great collection of authors and reviewers represents an interdisciplinary team of globally acknowledged experts. The book will therefore make a major contribution to establishing a common professional language, enhancing global communication between wastewater professionals. In addition, the authors have linked the description of the scientific basis for wastewater treatment processes with a video-based online course for the training of students, researchers, engineers, laboratory technicians and treatment plant operators, demonstrating commonly accepted experimentation procedures and their application for lab-, pilot-, and full-scale treatment plant operation.From the perspective of the IWA this book also has the great potential to enhance the development of a new generation of researchers and enable them to communicate on a global scale and beyond their specific field of expertise. Both aspects are urgently needed to develop adapted solutions for specific local conditions and to make them globally available for implementation.There has been a trend for some time that scientific research and practice have been growing apart from each other. Part of the rea...

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Monitoring of the degradation of aromatic hydrocarbons by bioaugmented activated sludge

Vural

Özdemir

2019

J of Chemical Tech & Biotech

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BACKGROUND: The petrochemical industry wastewater contains many organic compounds that are difficult to degrade. The activated sludge system reinforced by bioaugmentation technology represents a more effective alternative to treat industrial wastewater. In this study, bioaugmented activated sludge water demonstrated high efficiency in removing five tested hydrocarbons.RESULTS: Optimized degradation rates were obtained upon inoculation of a continuous bioreactor system with degradative bacteria (Acinetobacter sp., Aeromonas sp., Arthrobacter nicotianae, Burkholderia cepacia, Chryseobacterium sp., Pseudomonas fluorescens, Micrococcus luteus and Raoultella planticola). The addition of a hydrocarbon mixture (terephthalic acid, acenaphthene, fluorene, anthracene, and pyrene) into the bioaugmented sludge resulted in degradation rates equivalent to 97.90%, 99.87%, 98.89%, 97.28%, and 94.55% respectively after 24-48 h incubation. Molecular biomonitoring was performed with real-time quantitative polymerase chain reaction (qPCR). According to the obtained results, the numbers of copy DNA of the degradative bacteria increased by 0.36-1.13 log at the end of the degradation process. The presence of some oxygenase genes (Nah, NidA, Pah Rhd Gr(+), Pah Rhd Gr(−) and C23O), that catabolize aromatic hydrocarbons in activated sludge, were detected by specific primers. CONCLUSION: Bioaugmentation represents a potent and eco-friendly approach that can effectively remove the drastic increase in the concentrations of aromatic hydrocarbons in industrial wastewaters. Molecular biomonitoring during degradation can be considered a rapid and an effective in situ method that quantifies the active microbial population in wastewater. The method provides beneficial information about the composition of microbial community and sustainability of the degradation process. Figure 3. The graphics reveal the alterations in the copy number of DNA of bacterial isolates in bioaugmented sludge during the three stages (n = 3, ± standard deviation). (a-c) The numbers of bacteria at the first, second and third stages and the copy number of DNA in the first, second and third stages, respectively. The copy number of DNA of all bacterial isolates have increased as the concentration of hydrocarbon increased. J Chem Technol Biotechnol 2020; 95: 52-62

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Survival and activity of individual bioaugmentation strains

Cited by 56 publications

References 23 publications

Metagenomic insight into the bioaugmentation mechanism of Phanerochaete chrysosporium in an activated sludge system treating coking wastewater

Metagenomic insight into the bioaugmentation mechanism of Phanerochaete chrysosporium in an activated sludge system treating coking wastewater

Experimental Methods in Wastewater Treatment

Monitoring of the degradation of aromatic hydrocarbons by bioaugmented activated sludge

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