Surveying genetically modified maize in foods marketed in Algeria

Brara, Zahia; Costa, Joana; Villa, Caterina; Grazina, Liliana; Bitam, Arezki; Mafra, Isabel

doi:10.1016/j.foodcont.2019.106928

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…In many studies it is demonstrated that DNA isolation method can produce significantly different DNA yields and purity for different maize and soybean foodstuffs (Brara et al, 2020;Chakraborty et al, 2020;Stefanova et al, 2013;Turkec et al, 2015). Our observations are in accordance with previous studies, in which the commercial kits are generally considered the best for getting better results in both yield and purity for maize flour, maize starch, maize flakes (Rizzi et al, 2003;Smith and Maxwell, 2007;Peano et al, 2004).…”

Section: Dna Isolationsupporting

confidence: 91%

Investigating The GMO Existence in Chips and Breakfast Cereals Marketed in Turkey

Mutlu¹,

Şimşek

Öksüz

2021

Tekirdağ Ziraat Fakültesi Dergisi

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In this research, processed or low processed samples containing corn or corn products (corn semolina, flour, etc.) and soybean were randomly collected from the market, and 25 products in total (chips, nuts, cereals, flour) were analyzed for genetic modification using DNA based detection method, the polymerase chain reaction. First, homogenization of the samples was performed. Then DNA isolation was done by using Cetyl Trimethyl Ammonium Bromide (CTAB) and Roche High Pure DNA Isolation Kit. Since the Roche High Pure DNA Isolation Kit gave better results, the analysis was completed with this method. After DNA isolation, the detection of the Lectin gene, Zein gene, CaMV 35S Promoter and NOS Terminator regions was performed by conventional PCR. Zein gene determination was done for searching and proving corn presence and similarly, Lectin gene determination was done for searching and proving soybean presence in the samples by conventional PCR. GMO3/GMO4 and Zein3/Zein4 primer pairs were used for Lectin and Zein gene determination, respectively. The amplification of DNA was observed in agarose gel electrophoresis. Lectin or Zein genes were detected in 17 samples while these genes were not detected in 8 samples. Samples, in which Lectin or Zein gene was detected were scanned for 35S promoter or NOS terminator. 35S-3/35S-6 and tNOS2F/tNOS2R primer pairs were used for scanning 35S Promoter and NOS Terminator, respectively. To observe possible contamination in the mix sterilized deionized water was used and 0% Bt-11 and 0% GTS 40-3-2 were used as a negative control, 5% Bt-11 and 10% GTS-40-3-2 were used as a positive control. All of the 25 samples did not provide enough DNA with the required quality. This result was considered to be sourced by the applications (frying, extruding, pressing etc.) that samples had been exposed to during processes. Neither 35S Promotor nor NOS Terminator was determined from any of the samples.

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Section: Dna Isolationsupporting

confidence: 91%

Investigating The GMO Existence in Chips and Breakfast Cereals Marketed in Turkey

Mutlu¹,

Şimşek

Öksüz

2021

Tekirdağ Ziraat Fakültesi Dergisi

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“…The GM maize used for food is mainly used to extract maize oil [12], to make maize syrup, maize flour, or other maize ingredients [13], especially maize starch, which is widely used as a thickener, gelling agent, filler, and water-retention agent in the food industry [14]. GM maize used for food is also directly used as a raw material for food production and processing, such as in the production of tortillas in Mexico [15]; white maize in South Africa, where it is a staple food for most people [16], and maize flakes, popcorn, and maize-related snacks [17]. There is no doubt that GM maize is integrated into the lives of humans worldwide and it is, therefore, important to effectively regulate its production and processing.…”

Section: Introductionmentioning

confidence: 99%

Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize

Luo,

Li,

Wang

et al. 2023

IJMS

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Genetically modified (GM) maize is one of the earliest GM crops to have achieved large-scale commercial cultivation globally, and it is of great significance to excel in the development and implementation of safety policy regarding GM, and in its technical oversight. This article describes the general situation regarding genetically modified maize, including its varieties, applications, relevant laws and regulations, and so on. From a technical point of view, we summarize and critically analyze the existing methods for detecting nucleic acid levels in genetically modified maize. The nucleic acid extraction technology used for maize is explained, and the introduction of traditional detection techniques, which cover variable-temperature and isothermal amplification detection technology and gene chip technology, applications in maize are described. Moreover, new technologies are proposed, with special attention paid to nucleic acid detection methods using sensors. Finally, we review the current limitations and challenges of GM maize nucleic acid testing and share our vision for the future direction of this field.

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Surveying genetically modified maize in foods marketed in Algeria

Cited by 2 publications

References 31 publications

Investigating The GMO Existence in Chips and Breakfast Cereals Marketed in Turkey

Investigating The GMO Existence in Chips and Breakfast Cereals Marketed in Turkey

Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize

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