Survey on infection rate, vectors and molecular identification of Theileria annulata in cattle from North West, Iran

Yamchi, Jafar Arjmand; Tavassoli, Mousa

doi:10.1007/s12639-014-0636-y

Cited by 10 publications

(3 citation statements)

References 23 publications

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“…The acini of female tick SGs were more frequently T. annulata-infected than those of male ticks. This is consistent with the finding that female ticks have greater frequencies of Theileria infection (Yamchi and Tavassoli, 2016 [3]). The different feeding habits of male and female ticks may account for variation in infection rates.…”

Section: Discussionsupporting

confidence: 91%

“…(Ixodidae) have a remarkable capacity to survive in severe and difficult environments (Hasan et al, 2012 [2]). The majority of Hyalomma spp., including Hyalomma anatolicum, H. scupense, and H. lusitanicum, are known to transmit tropical theileriosis due to Theileria annulata (Piroplasmida: Theileriidae) (Yamchi and Tavassoli, 2016 [3]). Unfortunately, many factors have led to dangerously high levels of H. anatolicum in Pakistan (Rehman et al, 2017 [4]).…”

Section: Introductionmentioning

confidence: 99%

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Guardians of the Herd: Molecular Surveillance of Tick Vectors Uncovers Theileriosis Perils in Large Ruminants

Sajid,

Iqbal,

Rizwan

et al. 2023

Microorganisms

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Theileria sp. (Piroplasmida: Theileriidae) is one of the most widely known infections transmitted by hard ticks (Acari: Ixodidae) and has been linked to significant economic losses across the globe. The study’s main emphasis was theileriosis, a disease that is common in Pakistan and has an incidence ranging from 0.6% to 33%. Through DNA screening of the vector ticks and host blood, this study sought to determine the risk of tick-borne theileriosis in populations of buffalos (Bubalus bubalis) and cattle (Bos indicus) in Toba Tek Singh district of Punjab, Pakistan. Identified tick species include Hyalomma anatolicum (35.4%), Rhipicephalus (Boophilus) microplus (30.2%), and R. sanguineus (25%). Tick specimens were collected from animals and their respective microenvironments. PCR assays targeting Theileria annulata were used to investigate the infection in the DNA extracted from the collected blood samples from large ruminants and salivary glands (SGs) of the Hyalomma ticks. The 18S rRNA of T. annulata was amplified using specific primers. Positive T. annulata amplicons were sequenced and verified using BLAST analysis. Overall, 50% of SGs contained T. annulate DNA. Female ticks, and those collected from cattle and from riverine environments had significantly higher (p < 0.05) rates of Theileria infection in their acini. Overall prevalence of Theileria infection was 35.9% in blood collected from large ruminants. Cattle had a substantially greater frequency of bovine theileriosis (43.2%) than buffalos (28.7%). Age and sex of large ruminants were significantly positively associated (p < 0.05) with Theileria infection. Furthermore, compared to non-riverine cattle (35%) and buffalo (19.5%), riverine cattle (52.2%) and buffalo (36.2%) showed a considerably higher prevalence. The results of this study, which is the first in Pakistan to examine the blood of large ruminants and vectorial function of Ixodid ticks in the transmission of T. annulata along with associated risk factors, offer an important insight for risk assessment of Theileria infection in livestock using vectorial infectivity.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Guardians of the Herd: Molecular Surveillance of Tick Vectors Uncovers Theileriosis Perils in Large Ruminants

Sajid,

Iqbal,

Rizwan

et al. 2023

Microorganisms

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“…Moreover, the accurate diagnosis of piroplasms requires a wide range of laboratory techniques such as serological tests, blood smears and most definitely molecular diagnosis [13]. ELISA technique can be highly efficient in detecting both clinical and carrier animal, but lack specificity in determining recent and old infection [11].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection and Identification of Babesia spp., and Trypanosoma spp. in One-humped Camel (Camelus dromedarius) in Halayeb and Shalateen, Egypt

El‒Sayed¹,

El‐Adl²,

Ali³

et al. 2020

Preprint

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Phylogenetic analysis of blood parasite infections including Babesia (B.) bovis, Babesia microti and Trypanosoma (T.) spp. in one-humped camel (Camelus dromedarius) (n= 142) breeds in Halayeb and Shalateen, in Upper Egypt were performed in the current study. Polymerase chain reaction (PCR) assays targeting the Rhoptry Associated Protein-1 (RAP-1), Babesia microti small subunit rRNA (ss-rRNA) and internal transcribed spacer 1 (ITS1) genes were used to detect the prevalence of B. bovis, B. microti and Trypanosoma spp. in camels, respectively. Nested PCR assays were used for the detection of Babesia spp. (B. bovis and B. microti). While, KIN-multi species PCR reaction was employed to detect and identify trypanosome DNA in camels. B. microti was detected in (17/142) with infection rate (11.97 %). Sequencing and phylogenetic analyses revealed that B. microti detected in camel was closely related to the German strain in rats and voles in France. B. bovis was also detected in (4/142) with infection rate (2.81%). The sequence and phylogenetic analyses revealed that the isolated B. bovis was closely related to strains isolated from Argentine, USA and Brazil. Moreover, T. evansi was detected in (8/142) with infection rate (5.63%). Sequence and phylogenetic analyses revealed that isolated T. evansi was closely related to T. theileri that was detected from cattle in Brazil. This study provides the first evidence of B. microti in camel in Egypt and highlights the possible role of one-humped camels in maintaining the enzootic cycle of Babesia transmission in Egypt.

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Epidemiology and molecular characterization of Theileria annulata in ticks collected from cattle in the central part of Tamil Nadu, India

Velusamy,

Ponnudurai,

Alagesan

et al. 2023

Parasitol Res

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Survey on infection rate, vectors and molecular identification of Theileria annulata in cattle from North West, Iran

Cited by 10 publications

References 23 publications

Guardians of the Herd: Molecular Surveillance of Tick Vectors Uncovers Theileriosis Perils in Large Ruminants

Guardians of the Herd: Molecular Surveillance of Tick Vectors Uncovers Theileriosis Perils in Large Ruminants

Molecular Detection and Identification of Babesia spp., and Trypanosoma spp. in One-humped Camel (Camelus dromedarius) in Halayeb and Shalateen, Egypt

Epidemiology and molecular characterization of Theileria annulata in ticks collected from cattle in the central part of Tamil Nadu, India

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