Survey on Biofilm Production and Presence of Attachment Factors in Human Uropathogenic Strains of Escherichia coli

Abstract

Background: Escherichia coli is one of the most common causes of different infections. Biofilm structure allows the strains to persist on the biotic and abiotic surfaces for a long time and impairs eradication. Surface colonization of E. coli could be done with several extracellular appendages, which are effective productive events leading to biofilm maturity. Objectives: In this study, the possible relationship between the presence of fimA (encoding large subunit of Type I fimbriae) and csgA (encoding curli fimbriae) genes with biofilm formation in extraintestinal pathogen E. coli isolates was evaluated. Methods: For this study, 35 isolates of E. coli were collected from human urine samples of those referred to oil big hospital. After isolating and identifying E. coli strains by common biochemical tests, we examined the biofilm formation of isolates in brain heart infusion broth, which contained 3% sucrose, using microtiter plate crystal violet method. Presence of the 2 studied genes in the isolates was evaluated using multiplex polymerase chain reaction (m-PCR) assay. Results: In the present study, 27 strains from 35 isolates were expressed in the 2 studied attachment-associated factors, but 5 and 2 strains were expressed by csgA and fimA, respectively. Except 2 strains that could not produce biofilm, 1 strain was detected as a moderate biofilm producer, and the 32 remaining strains were detected as weak biofilm producers. Conclusions: All the positive and the 2 negative biofilm producer strains could be expressed in the 2 studied genes. The correlation between the presence of studied genes and biofilm production ability was suspected, but because of the high percentage of biofilm production in the studied strains, the need to use good hygiene practices is highly recommended.

Detection and Characterization of b-lactamases and Biofilm-producing Escherichia coli and Klebsiella pneumoniae from Ducks and Duck Environments in West Bengal

“…Approx. 27 E. coli and 13 K. pneumoniae isolates were found to possess more than one biofilm-producing gene in this study which was also supported by Bakhtiari and Javadmakoei (2017) and Banerjee and Acharyya (2020).…”

Evaluation of Biofilm Formation and Frequency of Genes Encoding Curli Fiber, Colanic Acid Capsule and F1c Fimberia Among Uropathogenic Escherichia coli Isolates With Strong Cell Surface Hydrophobicity

“…Based on the results of Qin, the prevalence of csgA genes in UPEC isolates and commensal E. coli isolates was 30% and 34%, respectively, indicating no significant differences between the isolates (43). In another study, Bakhtiari et al demonstrated that 33 out of 35 UPEC isolates had csgA gene while they found no correlation between the presence of csgA and biofilm production ability since most of the isolates with or without biofilm production ability had csgA gene (44). Similarly, Rijavec et alobserved no association between usp, papC, and sfa/foc virulence genes and biofilm production in pathogenic E. coli (45).…”

Biofilm-Producing Ability of Bovine Extraintestinal Pathogenic Escherichia coli and Its Correlation with Attachment Factors

“…They recommended the BHI medium containing 1% sucrose for the study of biofilmproducing ability of these strains (32). In a study by Rijavec et al biofilm production by pathogenic E. coli had no correlation with the presence of papC, usp, and sfa/foc virulence genes.…”